The circadian oscillator gene GIGANTEA mediates a long-term response of the Arabidopsis thaliana circadian clock to sucrose.

Circadian clocks are 24-h timing devices that phase cellular responses; coordinate growth, physiology, and metabolism; and anticipate the day-night cycle. Here we report sensitivity of the Arabidopsis thaliana circadian oscillator to sucrose, providing evidence that plant metabolism can regulate circadian function. We found that the Arabidopsis circadian system is particularly sensitive to sucrose in the dark. These data suggest that there is a feedback between the molecular components that comprise the circadian oscillator and plant metabolism, with the circadian clock both regulating and being regulated by metabolism. We used also simulations within a three-loop mathematical model of the Arabidopsis circadian oscillator to identify components of the circadian clock sensitive to sucrose. The mathematical studies identified GIGANTEA (GI) as being associated with sucrose sensing. Experimental validation of this prediction demonstrated that GI is required for the full response of the circadian clock to sucrose. We demonstrate that GI acts as part of the sucrose-signaling network and propose this role permits metabolic input into circadian timing in Arabidopsis.

The effect of boundary layer trips on intake separation in crosswinds

The flow typical of that occurring over the windward lip of an aero engine intake operating in a crosswind has been reproduced on a 2D lip. The uncontrolled boundary layer undergoes a laminar separation at the leading edge of the lip. It has been shown that a minimum size of boundary layer trip, positioned upstream of the separation location, is required to enable the flow to remain attached around the leading edge. A turbulent separation then occurs in the diffuser. Larger diameter trips promote earlier diffuser separation. Copyright © 2008 by the American Institute of Aeronautics and Astronautics, Inc.

Comparison between empirical formulae of intake vortices

The hydrodynamic properties of free surface vortices at hydraulic intakes were investigated. Based on the axisymmetric Navier-Stokes equations and empirical assumptions, two sets of formulations for the velocity distributions and the free surface profiles are proposed and validated against measurements available in the literature. Compared with previous formulae, the modifications based on Mih's formula are found to greatly improve the agreement with the experimental data. Physical model tests were also conducted to study the intake vortex of the Xiluodu hydroelectric project in China. The proposed velocity distribution formula was applied to the solid boundary as considered by the method of images. A good agreement was again observed between the prediction and the measurements. © 2011 International Association for Hydro-Environment Engineering and Research.