Isolating single primary rat hippocampal neurons and astrocytes on ultra-thin patterned parylene-C/silicon dioxide substrates

We report here the patterning of primary rat neurons and astrocytes from the postnatal hippocampus on ultra-thin parylene-C deposited on a silicon dioxide substrate, following observations of neuronal, astrocytic and nuclear coverage on strips of different lengths, widths and thicknesses. Neuronal and glial growth was characterized 'on', 'adjacent to' and 'away from' the parylene strips. In addition, the article reports how the same material combination can be used to isolate single cells along thin tracks of parylene-C. This is demonstrated with a series of high magnification images of the experimental observations for varying parylene strip widths and thicknesses. Thus, the findings demonstrate the possibility to culture cells on ultra-thin layers of parylene-C and localize single cells on thin strips. Such work is of interest and significance to the Neuroengineering and Multi-Electrode Array (MEA) communities, as it provides an alternative insulating material in the fabrication of embedded micro-electrodes, which can be used to facilitate single cell stimulation and recording in capacitive coupling mode. © 2010 Elsevier Ltd.

The use of energy in China: Tracing the flow of energy from primary source to demand drivers

We present a map of the transformation of energy in China as a Sankey diagram. After a review of previous work, and a statement of methodology, our main work has been the identification, evaluation, and treatment of appropriate data sources. This data is used to construct the Sankey diagram, in which flows of energy are traced from energy sources through end-use conversion devices, passive systems and final services to demand drivers. The resulting diagram provides a convenient and clear snapshot of existing energy transformations in China which can usefully be compared with a similar global analysis and which emphasises the potential for improvements in energy efficiency in 'passive systems'. More broadly, it gives a basis for examining and communicating future energy scenarios, including changes to demand, changes to the supply mix, changes in efficiency and alternative provision of existing services. © 2012 Elsevier Ltd.