Phase-variable surface structures are required for infection of Campylobacter jejuni by bacteriophages.

This study characterizes the interaction between Campylobacter jejuni and the 16 phages used in the United Kingdom typing scheme by screening spontaneous mutants of the phage-type strains and transposon mutants of the sequenced strain NCTC 11168. We show that the 16 typing phages fall into four groups based on their patterns of activity against spontaneous mutants. Screens of transposon and defined mutants indicate that the phage-bacterium interaction for one of these groups appears to involve the capsular polysaccharide (CPS), while two of the other three groups consist of flagellatropic phages. The expression of CPS and flagella is potentially phase variable in C. jejuni, and the implications of these findings for typing and intervention strategies are discussed.

Supporting excavations in clay - From analysis to decision-making

Finite Element Analysis (FEA) is used to calibrate a decision-making tool based on an extension of the Mobilized Strength Design (MSD) method which permits the designer an extremely simple method of predicting ground displacements during construction. This newly extended MSD approach accommodates a number of issues which are important in underground construction between in-situ walls, including: alternative base heave mechanisms suitable either for wide excavations in relatively shallow soft clay strata, or narrow excavations in relatively deep soft strata; the influence of support system stiffness in relation to the sequence of propping of the wall; and the capability of dealing with stratified ground. These developments should make it possible for a design engineer to take informed decisions on the relationship between prop spacing and ground movements, or the influence of wall stiffness, or on the need for and influence of a jet-grouted base slab, for example, without having to conduct project-specific FEA. © 2009 Taylor & Francis Group.

Fibre optic installation techniques for pile instrumentation

An innovative technique based on optical fibre sensing that allows continuous strain measurement has recently been introduced in structural health monitoring. Known as Brillouin Optical Time-Domain Reflectometry (BOTDR), this distributed optical fibre sensing technique allows measurement of strain along the full length (up to 10km) of a suitably installed optical fibre. Examples of recent implementations of BOTDR fibre optic sensing in piles are described in this paper. Two examples of distributed optical fibre sensing in piles are demonstrated using different installation techniques. In a load bearing pile, optical cables were attached along the reinforcing bars by equally spaced spot gluing to measure the axial response of pile to ground excavation induced heave and construction loading. Measurement of flexural behaviour of piles is demonstrated in the instrumentation of a secant piled wall where optical fibres were embedded in the concrete by simple endpoint clamping. Both methods have been verified via laboratory works. © 2009 IOS Press.

Experimental and numerical study of grout injections in silty soils

Compensation grouting is increasingly employed as a mitigation technique of settlements induced by tunnelling and its effectiveness both in clayey and sandy soils is reported in a wide number of case histories. However, the results are highly dependent on grout properties, injection characteristics and soil properties. An experimental study was conducted to investigate the parameters that control grout injections in silty soils. The results from one injection test in a large sample of silty soil show that the compensation efficiency, defined as the ratio of the volume of heave obtained at ground surface and the injected grout volume, is much lower than one and tends to decrease with time, while the initial volume of grout lost due to pressure filtration is small. Finally, results from finite elements back analyses of the laboratory test show that a good agreement with the experimental data can be obtained if the development of large strains is taken into account. © 2012 Taylor & Francis Group.