Accurate sodium bisulfite sequencing in plants.

DNA cytosine methylation is a conserved epigenetic modification frequently correlating with transcriptional silencing in a wide variety of eukaryotic organisms. Sodium bisulfite treatment of DNA converts unmethylated cytosine to uracil, while 5-methylated cytosine is protected. We describe techniques that ensure reliable sequencing data following sodium bisulfite conversion and to avoid common pitfalls such as amplification of unconverted DNA and inclusion of sibling clones.

3D multi-car tracking based on monocular video

We propose a system that can reliably track multiple cars in congested traffic environments. Our system's key basis is the implementation of a sequential Monte Carlo algorithm, which introduces robustness against problems arising due to the proximity between vehicles. By directly modelling occlusions and collisions between cars we obtain promising results on an urban traffic dataset. Extensions to this initial framework are also suggested. © 2010 IEEE.