1 resultado para Recombinant Proteins -- therapeutic use
em Cambridge University Engineering Department Publications Database
Filtro por publicador
- Aberdeen University (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (6)
- Aquatic Commons (1)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (10)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (1)
- Aston University Research Archive (26)
- B-Digital - Universidade Fernando Pessoa - Portugal (2)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (27)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (12)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (4)
- Biodiversity Heritage Library, United States (1)
- Bioline International (4)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (34)
- Brock University, Canada (5)
- CaltechTHESIS (1)
- Cambridge University Engineering Department Publications Database (1)
- CentAUR: Central Archive University of Reading - UK (14)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (10)
- Cochin University of Science & Technology (CUSAT), India (2)
- Coffee Science - Universidade Federal de Lavras (1)
- CORA - Cork Open Research Archive - University College Cork - Ireland (3)
- Deakin Research Online - Australia (34)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (35)
- Digital Commons - Michigan Tech (1)
- Digital Commons @ DU | University of Denver Research (1)
- Digital Commons at Florida International University (1)
- Digital Repository at Iowa State University (1)
- DigitalCommons@The Texas Medical Center (18)
- DigitalCommons@University of Nebraska - Lincoln (1)
- Doria (National Library of Finland DSpace Services) - National Library of Finland, Finland (1)
- DRUM (Digital Repository at the University of Maryland) (1)
- Duke University (24)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (2)
- Harvard University (2)
- Helda - Digital Repository of University of Helsinki (25)
- Indian Institute of Science - Bangalore - Índia (16)
- Instituto Politécnico de Leiria (1)
- Instituto Politécnico de Viseu (1)
- Instituto Politécnico do Porto, Portugal (1)
- Massachusetts Institute of Technology (2)
- National Center for Biotechnology Information - NCBI (56)
- Publishing Network for Geoscientific & Environmental Data (1)
- QSpace: Queen's University - Canada (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (40)
- Queensland University of Technology - ePrints Archive (107)
- ReCiL - Repositório Científico Lusófona - Grupo Lusófona, Portugal (1)
- Repositório do Centro Hospitalar de Lisboa Central, EPE - Centro Hospitalar de Lisboa Central, EPE, Portugal (15)
- Repositório Institucional da Universidade Estadual de São Paulo - UNESP (2)
- Repositório Institucional da Universidade Federal do Rio Grande do Norte (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (98)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (3)
- SAPIENTIA - Universidade do Algarve - Portugal (2)
- School of Medicine, Washington University, United States (1)
- Universidad de Alicante (2)
- Universidad del Rosario, Colombia (4)
- Universidad Politécnica de Madrid (1)
- Universidade de Lisboa - Repositório Aberto (4)
- Universidade Federal do Pará (3)
- Universidade Federal do Rio Grande do Norte (UFRN) (16)
- Universita di Parma (2)
- Universitat de Girona, Spain (5)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (2)
- Université de Lausanne, Switzerland (85)
- Université de Montréal, Canada (14)
- University of Connecticut - USA (1)
- University of Michigan (1)
- University of Queensland eSpace - Australia (28)
- University of Washington (1)
Resumo:
One of the commonly used resins for immobilized metal affinity purification of polyhistidine-tagged recombinant proteins is TALON resin, a cobalt (II)--carboxymethylaspartate-based matrix linked to Sepharose CL-6B. Here, we show that TALON resin efficiently purifies the native form of Lac repressor, which represents the major contaminant when (His)(6)-tagged proteins are isolated from Escherichia coli host cells carrying the lacI(q) gene. Inspection of the crystal structure of the repressor suggests that three His residues (residues 163, 173, and 202) in each subunit of the tetramer are optimally spaced on an exposed face of the protein to allow interaction with Co(II). In addition to establishing a more efficient procedure for purification of the Lac repressor, these studies indicate that non-lacI(q)-based expression systems yield significantly purer preparations of recombinant polyhistidine-tagged proteins.