Biofluid behaviour in 3D microchannel systems: Numerical analysis and design development of 3D microchannel biochip separators

This paper describes the design and development cycle of a 3D biochip separator and the modelling analysis of flow behaviour in the biochip microchannel features. The focus is on identifying the difference between 2D and 3D implementations as well as developing basic forms of 3D microfluidic separators. Five variants, based around the device are proposed and analysed. These include three variations of the branch channels (circular, rectangular, disc) and two variations of the main channel (solid and concentric). Ignoring the initial transient behaviour and assuming steady state flow has been established, the efficiencies of the flow between the main and side channels for the different designs are analysed and compared with regard to relevant biomicrofluidic laws or effects (bifurcation law, Fahraeus effect, cell-free phenomenon, bending channel effect and laminar flow behaviour). The modelling results identify flow features in microchannels, a constriction and bifurcations and show detailed differences in flow fields between the various designs. The manufacturing process using injection moulding for the initial base case design is also presented and discussed. The work reported here is supported as part of the UK funded 3D-MINTEGRATION project. © 2010 IEEE.

Spin glass behaviour and spin-dependent scattering in la 0.7Ca 0.3Mn 0.9Cr 0.1O 3 perovskites

The magnetic, electrical and thermal transport properties of the perovskite La 0.7Ca 0.3Mn 0.9Cr 0.1O 3 have been investigated by measuring dc magnetization, ac susceptibility, the magnetoresistance and thermal conductivity in the temperature range of 5-300K. The spin glass behaviour with a spin freezing temperature of 70 K has been well confirmed for this compound, which demonstrates the coexistence and competition between ferromagnetic and antiferromagnetic clusters by the introduction of Cr. Colossal magnetoresistance has been observed over the temperature range investigated. The introduction of Cr causes the "double-bump" feature in electrical resistivity ρ(T). Anomalies on the susceptibility and the thermal conductivity associated with the double-bumps in ρ(T) are observed simultaneously. The imaginary part of ac susceptibility shows a sharp peak at the temperature of insulating-metallic transition where the first resistivity bump was observed, but it is a deep-set valley near the temperature where the second bump in ρ(T) emerges. The thermal conductivity shows an increase below the temperature of the insulating-metallic transition, but the phonon scattering is enhanced accompanying the appearance of the second peak of double-bumps in ρ(T). We relate those observed in magnetic and transport properties of La 0.7Ca 0.3Mn 0.9Cr 0.1O 3 to the spin-dependent scattering. The results reveal that the spin-phonon interaction may be of more significance than the electron (charge)-phonon interaction in the mixed perovskite system. © 2005 Chinese Physical Society and IOP Publishing Ltd.

Improving productivity - Opening the black box

Hourly productivity levels in the UK still remain behind those in some competitor countries. The government devotes much policy attention to enhancing productivity and continues to emphasise its five drivers - investment, innovation, skills, enterprise, and competition. This article argues that it is investment broadly defined that is the key to sustained productivity improvement. The emphasis should be on improving productivity simultaneously with improving the quality of production. Only thus will the gains be widely shared. In achieving these aims there are two prerequisites for policy-makers. The first is to ensure better coordination of policy than appears to be currently achieved by the present departmental structures in Whitehall. The second is to recognize fully the long and complex chain of causation that can be triggered by pulling on one policy lever. Such complexity can only be fully understood by more research on what actually goes on inside the black box of the organization. © 2006 Oxford University Press.

Epigenetic remodeling of meiotic crossover frequency in Arabidopsis thaliana DNA methyltransferase mutants.

Meiosis is a specialized eukaryotic cell division that generates haploid gametes required for sexual reproduction. During meiosis, homologous chromosomes pair and undergo reciprocal genetic exchange, termed crossover (CO). Meiotic CO frequency varies along the physical length of chromosomes and is determined by hierarchical mechanisms, including epigenetic organization, for example methylation of the DNA and histones. Here we investigate the role of DNA methylation in determining patterns of CO frequency along Arabidopsis thaliana chromosomes. In A. thaliana the pericentromeric regions are repetitive, densely DNA methylated, and suppressed for both RNA polymerase-II transcription and CO frequency. DNA hypomethylated methyltransferase1 (met1) mutants show transcriptional reactivation of repetitive sequences in the pericentromeres, which we demonstrate is coupled to extensive remodeling of CO frequency. We observe elevated centromere-proximal COs in met1, coincident with pericentromeric decreases and distal increases. Importantly, total numbers of CO events are similar between wild type and met1, suggesting a role for interference and homeostasis in CO remodeling. To understand recombination distributions at a finer scale we generated CO frequency maps close to the telomere of chromosome 3 in wild type and demonstrate an elevated recombination topology in met1. Using a pollen-typing strategy we have identified an intergenic nucleosome-free CO hotspot 3a, and we demonstrate that it undergoes increased recombination activity in met1. We hypothesize that modulation of 3a activity is caused by CO remodeling driven by elevated centromeric COs. These data demonstrate how regional epigenetic organization can pattern recombination frequency along eukaryotic chromosomes.

Multi-objective shape optimisation for horizontal-axis wind turbine blades

We are developing a wind turbine blade optimisation package CoBOLDT (COmputa- tional Blade Optimisation and Load De ation Tool) for the optimisation of large horizontal- axis wind turbines. The core consists of the Multi-Objective Tabu Search (MOTS), which controls a spline parameterisation module, a fast geometry generation and a stationary Blade Element Momentum (BEM) code to optimise an initial wind turbine blade design. The objective functions we investigate are the Annual Energy Production (AEP) and the fl apwise blade root bending moment (MY0) for a stationary wind speed of 50 m/s. For this task we use nine parameters which define the blade chord, the blade twist (4 parameters each) and the blade radius. Throughout the optimisation a number of binary constraints are defined to limit the noise emission, to allow for transportation on land and to control the aerodynamic conditions during all phases of turbine operation. The test case shows that MOTS is capable to find enhanced designs very fast and eficiently and will provide a rich and well explored Pareto front for the designer to chose from. The optimised blade de- sign could improve the AEP of the initial blade by 5% with the same flapwise root bending moment or reduce MY0 by 7.5% with the original energy yield. Due to the fast runtime of order 10 seconds per design, a huge number of optimisation iterations is possible without the need for a large computing cluster. This also allows for increased design flexibility through the introduction of more parameters per blade function or parameterisation of the airfoils in future. © 2012 by Nordex Energy GmbH.

Multi-objective shape optimisation for horizontal-axis wind turbine blades

We are developing a wind turbine blade optimisation package CoBOLDT (COmputa- tional Blade Optimisation and Load Deation Tool) for the optimisation of large horizontal- axis wind turbines. The core consists of the Multi-Objective Tabu Search (MOTS), which controls a spline parameterisation module, a fast geometry generation and a stationary Blade Element Momentum (BEM) code to optimise an initial wind turbine blade design. The objective functions we investigate are the Annual Energy Production (AEP) and the apwise blade root bending moment (MY0) for a stationary wind speed of 50 m/s. For this task we use nine parameters which define the blade chord, the blade twist (4 parameters each) and the blade radius. Throughout the optimisation a number of binary constraints are defined to limit the noise emission, to allow for transportation on land and to control the aerodynamic conditions during all phases of turbine operation. The test case shows that MOTS is capable to find enhanced designs very fast and efficiently and will provide a rich and well explored Pareto front for the designer to chose from. The optimised blade de- sign could improve the AEP of the initial blade by 5% with the same apwise root bending moment or reduce MY0 by 7.5% with the original energy yield. Due to the fast runtime of order 10 seconds per design, a huge number of optimisation iterations is possible without the need for a large computing cluster. This also allows for increased design flexibility through the introduction of more parameters per blade function or parameterisation of the airfoils in future. © 2012 AIAA.