Experimental behaviour of barrel vaulted structure subjected to rocking of the abutments

In this paper, the transverse rocking mechanism of a barrel vaulted structure subjected to horizontal cyclic loads is analysed by means of experimental tests on full scale model and by means of non-linear FE analyses. The study is part of an ongoing experimental and theoretical research program, developed by the University of Brescia, concerning the seismic behaviour of ancient masonry buildings. The scope of the paper is to provide some evidence of the rocking mechanism experienced by barrel vaulted structures under horizontal loading. The understanding of the behaviour of these structural systems is necessary for their seismic vulnerability assessment, as well as for the correct design of possible strengthening techniques. A numeric FE model was validated through comparison with the experimental results and it was used to verify the efficiency of two common strengthening solutions: the technique of the overlaying reinforced concrete slab and the technique of the thin spandrel walls. Experimental and numeric results will be discussed in the paper.

An analysis of the cooperative mechano-sensitive feedback between intracellular signaling, focal adhesion development, and stress fiber contractility

Cells communicate with their external environment via focal adhesions and generate activation signals that in turn trigger the activity of the intracellular contractile machinery. These signals can be triggered by mechanical loading that gives rise to a cooperative feedback loop among signaling, focal adhesion formation, and cytoskeletal contractility, which in turn equilibrates with the applied mechanical loads. We devise a signaling model that couples stress fiber contractility and mechano-sensitive focal adhesion models to complete this above mentioned feedback loop. The signaling model is based on a biochemical pathway where IP3 molecules are generated when focal adhesions grow. These IP3 molecules diffuse through the cytosol leading to the opening of ion channels that disgorge Ca2+ from the endoplasmic reticulum leading to the activation of the actin/myosin contractile machinery. A simple numerical example is presented where a one-dimensional cell adhered to a rigid substrate is pulled at one end, and the evolution of the stress fiber activation signal, stress fiber concentrations, and focal adhesion distributions are investigated. We demonstrate that while it is sufficient to approximate the activation signal as spatially uniform due to the rapid diffusion of the IP3 through the cytosol, the level of the activation signal is sensitive to the rate of application of the mechanical loads. This suggests that ad hoc signaling models may not be able to capture the mechanical response of cells to a wide range of mechanical loading events. © 2011 American Society of Mechanical Engineers.