1 resultado para Adult development
em Cambridge University Engineering Department Publications Database
Filtro por publicador
- JISC Information Environment Repository (1)
- Repository Napier (1)
- University of Cagliari UniCA Eprints (1)
- Abertay Research Collections - Abertay University’s repository (1)
- Academic Archive On-line (Stockholm University; Sweden) (1)
- Academic Research Repository at Institute of Developing Economies (1)
- Acceda, el repositorio institucional de la Universidad de Las Palmas de Gran Canaria. España (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (2)
- Aquatic Commons (9)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (1)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (3)
- Aston University Research Archive (21)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (20)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (10)
- Biblioteca Digital de la Universidad del Valle - Colombia (1)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (1)
- Bioline International (1)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (40)
- Boston University Digital Common (1)
- Brock University, Canada (10)
- Bucknell University Digital Commons - Pensilvania - USA (4)
- CaltechTHESIS (2)
- Cambridge University Engineering Department Publications Database (1)
- CentAUR: Central Archive University of Reading - UK (37)
- Central European University - Research Support Scheme (1)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (11)
- Cochin University of Science & Technology (CUSAT), India (1)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (1)
- CORA - Cork Open Research Archive - University College Cork - Ireland (6)
- Dalarna University College Electronic Archive (1)
- Deakin Research Online - Australia (51)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (1)
- Digital Commons at Florida International University (23)
- DigitalCommons - The University of Maine Research (1)
- DigitalCommons@The Texas Medical Center (17)
- DigitalCommons@University of Nebraska - Lincoln (1)
- DRUM (Digital Repository at the University of Maryland) (1)
- Duke University (14)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (9)
- Glasgow Theses Service (3)
- Greenwich Academic Literature Archive - UK (4)
- Helda - Digital Repository of University of Helsinki (10)
- Indian Institute of Science - Bangalore - Índia (10)
- Institutional Repository of Leibniz University Hannover (2)
- Instituto Politécnico de Leiria (1)
- Instituto Politécnico de Viseu (1)
- Instituto Politécnico do Porto, Portugal (1)
- Instituto Superior de Psicologia Aplicada - Lisboa (1)
- Memorial University Research Repository (1)
- Ministerio de Cultura, Spain (1)
- National Center for Biotechnology Information - NCBI (40)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (2)
- Portal de Revistas Científicas Complutenses - Espanha (1)
- Publishing Network for Geoscientific & Environmental Data (6)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (36)
- Queensland University of Technology - ePrints Archive (374)
- RCAAP - Repositório Científico de Acesso Aberto de Portugal (1)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (2)
- Repositório Institucional da Universidade Federal de São Paulo - UNIFESP (1)
- Repositório Institucional da Universidade Federal do Rio Grande do Norte (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (77)
- Research Open Access Repository of the University of East London. (1)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (4)
- SAPIENTIA - Universidade do Algarve - Portugal (1)
- Universidad Politécnica de Madrid (3)
- Universidade Federal do Pará (3)
- Universidade Federal do Rio Grande do Norte (UFRN) (3)
- Universitat de Girona, Spain (2)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (1)
- Université de Lausanne, Switzerland (7)
- Université de Montréal (1)
- Université de Montréal, Canada (3)
- University of Connecticut - USA (2)
- University of Michigan (6)
- University of Queensland eSpace - Australia (17)
- University of Washington (2)
Resumo:
We have investigated whether inkjet printing technology can be extended to print cells of the adult rat central nervous system (CNS), retinal ganglion cells (RGC) and glia, and the effects on survival and growth of these cells in culture, which is an important step in the development of tissue grafts for regenerative medicine, and may aid in the cure of blindness. We observed that RGC and glia can be successfully printed using a piezoelectric printer. Whilst inkjet printing reduced the cell population due to sedimentation within the printing system, imaging of the printhead nozzle, which is the area where the cells experience the greatest shear stress and rate, confirmed that there was no evidence of destruction or even significant distortion of the cells during jet ejection and drop formation. Importantly, the viability of the cells was not affected by the printing process. When we cultured the same number of printed and non-printed RGC/glial cells, there was no significant difference in cell survival and RGC neurite outgrowth. In addition, use of a glial substrate significantly increased RGC neurite outgrowth, and this effect was retained when the cells had been printed. In conclusion, printing of RGC and glia using a piezoelectric printhead does not adversely affect viability and survival/growth of the cells in culture. Importantly, printed glial cells retain their growth-promoting properties when used as a substrate, opening new avenues for printed CNS grafts in regenerative medicine.