Dissecting Arabidopsis thaliana DICER function in small RNA processing, gene silencing and DNA methylation patterning.

Small RNAs have several important biological functions. MicroRNAs (miRNAs) and trans-acting small interfering RNAs (tasiRNAs) regulate mRNA stability and translation, and siRNAs cause post-transcriptional gene silencing of transposons, viruses and transgenes and are important in both the establishment and maintenance of cytosine DNA methylation. Here, we study the role of the four Arabidopsis thaliana DICER-LIKE genes (DCL1-DCL4) in these processes. Sequencing of small RNAs from a dcl2 dcl3 dcl4 triple mutant showed markedly reduced tasiRNA and siRNA production and indicated that DCL1, in addition to its role as the major enzyme for processing miRNAs, has a previously unknown role in the production of small RNAs from endogenous inverted repeats. DCL2, DCL3 and DCL4 showed functional redundancy in siRNA and tasiRNA production and in the establishment and maintenance of DNA methylation. Our studies also suggest that asymmetric DNA methylation can be maintained by pathways that do not require siRNAs.

Machine vision based concrete surface quality assessment

Manually inspecting concrete surface defects (e.g., cracks and air pockets) is not always reliable. Also, it is labor-intensive. In order to overcome these limitations, automated inspection using image processing techniques was proposed. However, the current work can only detect defects in an image without the ability of evaluating them. This paper presents a novel approach for automatically assessing the impact of two common surface defects (i.e., air pockets and discoloration). These two defects are first located using the developed detection methods. Their attributes, such as the number of air pockets and the area of discoloration regions, are then retrieved to calculate defects’ visual impact ratios (VIRs). The appropriate threshold values for these VIRs are selected through a manual rating survey. This way, for a given concrete surface image, its quality in terms of air pockets and discoloration can be automatically measured by judging whether their VIRs are below the threshold values or not. The method presented in this paper was implemented in C++ and a database of concrete surface images was tested to validate its performance. Read More: http://ascelibrary.org/doi/abs/10.1061/%28ASCE%29CO.1943-7862.0000126?journalCode=jcemd4