Inoculation of Triatoma Virus (Dicistroviridae: Cripavirus) elicits a non-infective immune response in mice

Background: Dicistroviridae is a new family of small, non-enveloped, +ssRNA viruses pathogenic to both beneficial arthropods and insect pests. Little is known about the dicistrovirus replication mechanism or gene function, and any knowledge on these subjects comes mainly from comparisons with mammalian viruses from the Picornaviridae family. Due to its peculiar genome organization and characteristics of the per os viral transmission route, dicistroviruses make good candidates for use as biopesticides. Triatoma virus (TrV) is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of the human trypanosomiasis disease called Chagas disease. TrV was postulated as a potential control agent against Chagas' vectors. Although there is no evidence that TrV nor other dicistroviruses replicate in species outside the Insecta class, the innocuousness of these viruses in humans and animals needs to be ascertained. Methods: In this study, RT-PCR and ELISA were used to detect the infectivity of this virus in Mus musculus BALB/c mice. Results: In this study we have observed that there is no significant difference in the ratio IgG2a/IgG1 in sera from animals inoculated with TrV when compared with non-inoculated animals or mice inoculated only with non-infective TrV protein capsids. Conclusions: We conclude that, under our experimental conditions, TrV is unable to replicate inmice. This study constitutes the first test to evaluate the infectivity of a dicistrovirus in a vertebrate animal model.

Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification

Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.

Familia eredu berriak haur hezkuntzan. Ikastetxe konfesional baten analisia.

[eus] Gradu Amaierako Lan honek Haur Hezkuntzako etapan familia aniztasunari ematen zaion trataera aztertzea du helburu. Horretarako Bilboko Elizbarrutiko ikastetxe zehatz batean kasu ikerketa bat egitea erabaki da. Azterketaren ardatza ikastetxearen izaera konfesionala familia eredu berrien aniztasuna lantzeko zenbateraino traba izan litekeen frogatzea izan da. Ikerketarako erabilitako metodologia kualitatiboa izan da, eztabaida taldea, elkarrizketa sakonak, behaketak eta edukien analisien bidezko teknikak nagusiak izanik. Teknika horien bitartez lortutako informazioaren inguruan zenbait ondorio atera dira eta, azkenik, horiek guztiak kontuan harturik hobekuntzarako proposamena planteatu da.

Basque legacy in the New World: on the surnames of Latin American presidents

[EN] In this article we explain the etymology of the surnames of Basque origin that some presidents of Latin American countries have or have had in the past. These family names were created in the language called Euskara, in the Basque Country (Europe), and then, when some of the people who bore them emigrated to America, they brought their surnames with them. Most of the family names studied here are either oiconymic or toponymic, but it must be kept in mind that the oiconymic ones are, very often, based on house-nicknames, that is, they are anthroponymic in the first place. As far as possible, we have related the surname, when its origin is oiconymic or toponymic, to its source, i.e. to the house or place where it was created.