Randomized, double-blind, placebo-controled clinical trial of sublingual immunotherapy in natural rubber latex allergic patients

Trial registration number: CTRN12611000543987

Magnetic Cross-Linked Enzyme Aggregates (mCLEAs) of Candida antarctica Lipase: An Efficient and Stable Biocatalyst for Biodiesel Synthesis

Enzyme-catalyzed production of biodiesel is the object of extensive research due to the global shortage of fossil fuels and increased environmental concerns. Herein we report the preparation and main characteristics of a novel biocatalyst consisting of Cross-Linked Enzyme Aggregates (CLEAs) of Candida antarctica lipase B (CALB) which are covalently bound to magnetic nanoparticles, and tackle its use for the synthesis of biodiesel from non-edible vegetable and waste frying oils. For this purpose, insolubilized CALB was covalently cross-linked to magnetic nanoparticles of magnetite which the surface was functionalized with –NH2 groups. The resulting biocatalyst combines the relevant catalytic properties of CLEAs (as great stability and feasibility for their reutilization) and the magnetic character, and thus the final product (mCLEAs) are superparamagnetic particles of a robust catalyst which is more stable than the free enzyme, easily recoverable from the reaction medium and reusable for new catalytic cycles. We have studied the main properties of this biocatalyst and we have assessed its utility to catalyze transesterification reactions to obtain biodiesel from non-edible vegetable oils including unrefined soybean, jatropha and cameline, as well as waste frying oil. Using 1% mCLEAs (w/w of oil) conversions near 80% were routinely obtained at 30°C after 24 h of reaction, this value rising to 92% after 72 h. Moreover, the magnetic biocatalyst can be easily recovered from the reaction mixture and reused for at least ten consecutive cycles of 24 h without apparent loss of activity. The obtained results suggest that mCLEAs prepared from CALB can become a powerful biocatalyst for application at industrial scale with better performance than those currently available.

Kautxu birziklatuzko materialek inpaktura duten erantzunaren simulazioa.

[EU]Ondorengo lanean ingeniaritzaren eremuan aurkitzen den eginkizunik garrantzitsuenetako bat egingo da: material berri bat karakterizatu. Hau da, errealitatean ikusten duguna era matematiko batean idatzi, edo beste era batean esanda, modelo matematiko bat lortu. Jorratuko den materiala kautxu birziklatua izango da, pneumatiko zaharretatik abiatuz egiten dena. Material honek, besteak beste, hurrengo abantailak ditu: produzitzeko erraza (beraz, prezio ekonomikoak, lehengaiak hondakin solidoak direla ikusita) eta inpakturako erantzun ona. Kautxu hau, beste erabileren artean, segurtasun-errailetan erabiltzen da, moto-gidariak ebaketetatik babesteko. Modelo matematikoa edo konstitutiboa lortzeko, lege hiperelastiko eta biskoelastikoetatik abiatuz, portaera bisko-hiperelastikorako lege bat proposatuko da. Lege hau deformazio handietarako eta deformazio abiadura handietarako aplikatu egingo da. Honen oinarria aurreko urteetan eskola honetako beste ikasleek eginiko gradu eta karrera amaierako lanak izango dira. Behin hau izanda, elementu finituetako software baten bidez simulatuko da, eta emaitza numerikoak esperimentalekin alderatuko dira. Honen bidez, modeloa hobetu ahal izango dugu, zehaztasun gehiago lortzeko asmoz.