Amplicon metagenomics for elucidating microbial community changes associated to conventional and organic farming of Beta vulgaris and Solanum lycopersicum

Soil microbial community changes associated to conventional and organic farming of two relevant crops (Beta vulgaris and Solanum lycopersicum) were analysed through 16s rRNA amplicon sequencing. This study revealed microbial communities in the agricultural soils studied to be similar to other reported nutrient-rich microbiomes, and some significant differences between the microbial communities associated to the two farming practices were found. Some phyla (Chloroflexi and Thermi) were found to be present in different abundances according to soil treatment. As chloroplast interference can be a stumbling block in plant-associated 16s rRNA amplicon metagenomics analysis of aerial plant tissues, two protocols for bacterial cell detachment (orbital shaking and ultrasound treatment) and two protocols for microbial biomass recovery (centrifugation and filtration) were tested regarding their efficiency at excluding plant-DNA. An alternative method to the one proposed by Rastogi et al (2010) for evaluating the chloroplast-amplicon content in post-PCR samples was tested, and the method revealed that filtration was the most efficient protocol in minimising chloroplast interference.

Basoa Euskal Herriko historian zehar

[EUS]Antzinako errejimenean basoa bizitzaren bizigunea zen.Garai honetan jarduera gehienak basoaren inguruan kokatzen ziren: gizakumeen elikadura, abereen janaria, ehiza,etxeko sua, ikatzaren industria,eraikinak, nekazaritza, etxeko eta teknika tresnak, ontzigintza, e.a. Horregati, basoak izan zuen bilakaera jarduera hauekin oso lotuta zegoen. Gipuzkoa eta Bizkaia jarduera hauen arteko eta basoaren inguruko aurkakotasuna bortitza baino bortitzagoa izan zuen. Egun, gauzak aldatu dira, basoak ez du ekonomiarekiko hainbeste garrantzirik. Momentu honetan basoarekiko interesa izadiaren kontserbatzioan eta suntsiketan datza

Transformaciones agropecuarias en Guipúzcoa durante los siglos XVI y XVII

Actas de la XII Reunión Científica de la Fundación Española de Historia Moderna, celebrada en la Universidad de León en 19-21 de junio de 2012.

Squaring the circle. Social and environmental implications of pre-pottery neolithic building technology at Tell Qarassa (South Syria)

We present the results of the microstratigraphic, phytolith and wood charcoal study of the remains of a 10.5 ka roof. The roof is part of a building excavated at Tell Qarassa (South Syria), assigned to the Pre-Pottery Neolithic B period (PPNB). The Pre-Pottery Neolithic (PPN) period in the Levant coincides with the emergence of farming. This fundamental change in subsistence strategy implied the shift from mobile to settled aggregated life, and from tents and huts to hard buildings. As settled life spread across the Levant, a generalised transition from round to square buildings occurred, that is a trademark of the PPNB period. The study of these buildings is fundamental for the understanding of the ever-stronger reciprocal socio-ecological relationship humans developed with the local environment since the introduction of sedentism and domestication. Descriptions of buildings in PPN archaeological contexts are usually restricted to the macroscopic observation of wooden elements (posts and beams) and mineral components (daub, plaster and stone elements). Reconstructions of microscopic and organic components are frequently based on ethnographic analogy. The direct study of macroscopic and microscopic, organic and mineral, building components performed at Tell Qarassa provides new insights on building conception, maintenance, use and destruction. These elements reflect new emerging paradigms in the relationship between Neolithic societies and the environment. A square building was possibly covered here with a radial roof, providing a glance into a topologic shift in the conception and understanding of volumes, from round-based to square-based geometries. Macroscopic and microscopic roof components indicate buildings were conceived for year-round residence rather than seasonal mobility. This implied performing maintenance and restoration of partially damaged buildings, as well as their adaptation to seasonal variability

Diseño, implementación y evaluación de material didáctico para Educación Primaria en relación a una visita a una explotación agropecuaria

105 p. : il. - Ilustraciones de Oscar Mardones Ruiz

Effects of international trade of food and feed and human diet shifts on food security and environmental safety: integrating scales

4 p.

Development of a new modelling framework to estimate the C footprint from Basque dairy farms

19 p.

Modelling the Effect of Climate Change on Environmental Pollution Losses from Dairy Systems in the UK

21 p.

Baserrietara egindako eskola bisitak: lehen hezkuntzarako material didaktikoen diseinua, ezarpena eta ebaluazioa

58 p.