NMDA modulates oligodendrocyte differentiation of subventricular zone cells through PKC activation

7 p.

Resveratrol prevents inflammation-dependent hepatic melanoma metastasis by inhibiting the secretion and effects of interleukin-18

Background: Implantation and growth of metastatic cancer cells at distant organs is promoted by inflammation-dependent mechanisms. A hepatic melanoma metastasis model where a majority of metastases are generated via interleukin-18-dependent mechanisms was used to test whether anti-inflammatory properties of resveratrol can interfere with mechanisms of metastasis. Methods: Two experimental treatment schedules were used: 1) Mice received one daily oral dose of 1 mg/kg resveratrol after cancer cell injection and the metastasis number and volume were determined on day 12. 2) Mice received one daily oral dose of 1 mg/kg resveratrol along the 5 days prior to the injection of cancer cells and both interleukin-18 (IL-18) concentration in the hepatic blood and microvascular retention of luciferase-transfected B16M cells were determined on the 18(th) hour. In vitro, primary cultured hepatic sinusoidal endothelial cells were treated with B16M-conditioned medium to mimic their in vivo activation by tumor-derived factors and the effect of resveratrol on IL-18 secretion, on vascular cell adhesion molecule-1 (VCAM-1) expression and on tumor cell adhesion were studied. The effect of resveratrol on melanoma cell activation by IL-18 was also studied. Results: Resveratrol remarkably inhibited hepatic retention and metastatic growth of melanoma cells by 50% and 75%, respectively. The mechanism involved IL-18 blockade at three levels: First, resveratrol prevented IL-18 augmentation in the blood of melanoma cell-infiltrated livers. Second, resveratrol inhibited IL-18-dependent expression of VCAM-1 by tumor-activated hepatic sinusoidal endothelium, preventing melanoma cell adhesion to the microvasculature. Third, resveratrol inhibited adhesion-and proliferation-stimulating effects of IL-18 on metastatic melanoma cells through hydrogen peroxide-dependent nuclear factor-kappaB translocation blockade on these cells. Conclusions: These results demonstrate multiple sites for therapeutic intervention using resveratrol within the prometastatic microenvironment generated by tumor-induced hepatic IL-18, and suggest a remarkable effect of resveratrol in the prevention of inflammation-dependent melanoma metastasis in the liver.

Bideratze arazoen ebazpenerako aplikatutako algoritmo metaheuristikoen diseinu, inplementazio eta konparaketa.

[EU]Proiektu honen helburua sare ezberdinetan algoritmo metaheuristikoen erabileraren bitartez bideratze arazoak ebaztea eta aztertzea da. Helburu honetarako erabiliko diren algoritmoak Coral Reefs Optimization eta Firefly Algorithm dira. Bi algoritmoak Python erabiliz inplementatuko dira, baita sareak simulatzen dituen programa ere. Modu honetan, algoritmo bakoitzaren gaitasuna aztertuko da sareko bi punturen arteko bide bideragarri bat, zeinek ezarritako murrizketak betetzen dituen, aurkitzeko; prozesu hau ausaz sortutako simulatutako sare batean oinarrituz garatuko da. Honen bitartez, arazo honen ebazpenerako algoritmo bakoitza egokia den eta bietariko zein den egokiena ondorioztatu ahalko da.

Cuando el miedo invade la ficción. Análisis de Perdidos (Lost) y de otros quality dramas de la era Post 11S.

521 p.