Ensayos NDT de piezas fabricadas por Láser Cladding.

[ES]Este trabajo consiste en realizar ensayos NDT de Líquidos Penetrantes sobre unas piezas fabricadas por Láser Cladding. Su objetivo es comprobar la integridad superficial y verificar si se producen discontinuidades superficiales relevantes, de modo que se pueda comprobar la calidad superficial que se obtiene en piezas fabricadas por este proceso.

Estudio experimental del aporte de material en forma de hilo por Laser Cladding

La competencia y la constante evolución del mercado de la Fabricación Aditiva, y en concreto del Laser Cladding, como en cualquier otro mercado industrial, demandan una alta productividad y una buena calidad de los recubrimientos. El objetivo principal de este trabajo, realizado en el Taller de Ingeniería Mecánica de la Universidad del País Vasco, es analizar estos dos factores basándose en el aporte de material en forma de hilo, y hacer una comparación de los resultados que se obtendrían con el aporte de polvo. Como indicador de la productividad se ha utilizado la tasa de aporte, y para analizar la calidad del aporte se han observado los defectos y el crecimiento de los cordones tanto en altura como en anchura. Otros factores como la dilución y la reducción de costes, también se han tenido en cuenta.

A neural extracellular matrix-based method for in vitro hippocampal neuron culture and dopaminergic differentiation of neural stem cells

Background: The ability to recreate an optimal cellular microenvironment is critical to understand neuronal behavior and functionality in vitro. An organized neural extracellular matrix (nECM) promotes neural cell adhesion, proliferation and differentiation. Here, we expanded previous observations on the ability of nECM to support in vitro neuronal differentiation, with the following goals: (i) to recreate complex neuronal networks of embryonic rat hippocampal cells, and (ii) to achieve improved levels of dopaminergic differentiation of subventricular zone (SVZ) neural progenitor cells. Methods: Hippocampal cells from E18 rat embryos were seeded on PLL- and nECM-coated substrates. Neurosphere cultures were prepared from the SVZ of P4-P7 rat pups, and differentiation of neurospheres assayed on PLL- and nECM-coated substrates. Results: When seeded on nECM-coated substrates, both hippocampal cells and SVZ progenitor cells showed neural expression patterns that were similar to their poly-L-lysine-seeded counterparts. However, nECM-based cultures of both hippocampal neurons and SVZ progenitor cells could be maintained for longer times as compared to poly-L-lysine-based cultures. As a result, nECM-based cultures gave rise to a more branched neurite arborization of hippocampal neurons. Interestingly, the prolonged differentiation time of SVZ progenitor cells in nECM allowed us to obtain a purer population of dopaminergic neurons. Conclusions: We conclude that nECM-based coating is an efficient substrate to culture neural cells at different stages of differentiation. In addition, neural ECM-coated substrates increased neuronal survival and neuronal differentiation efficiency as compared to cationic polymers such as poly-L-lysine.