The Oxidative State of Chylomicron Remnants Influences Their Modulation of Human Monocyte Activation

8 p.

The Expanded mtDNA Phylogeny of the Franco-Cantabrian Region Upholds the Pre-Neolithic Genetic Substrate of Basques

9 p.

Alcohol-Related Brain Damage in Humans

Chronic excessive alcohol intoxications evoke cumulative damage to tissues and organs. We examined prefrontal cortex (Brodmann's area (BA) 9) from 20 human alcoholics and 20 age, gender, and postmortem delay matched control subjects. H & E staining and light microscopy of prefrontal cortex tissue revealed a reduction in the levels of cytoskeleton surrounding the nuclei of cortical and subcortical neurons, and a disruption of subcortical neuron patterning in alcoholic subjects. BA 9 tissue homogenisation and one dimensional polyacrylamide gel electrophoresis (PAGE) proteomics of cytosolic proteins identified dramatic reductions in the protein levels of spectrin beta II, and alpha- and beta-tubulins in alcoholics, and these were validated and quantitated by Western blotting. We detected a significant increase in a-tubulin acetylation in alcoholics, a non-significant increase in isoaspartate protein damage, but a significant increase in protein isoaspartyl methyltransferase protein levels, the enzyme that triggers isoaspartate damage repair in vivo. There was also a significant reduction in proteasome activity in alcoholics. One dimensional PAGE of membrane-enriched fractions detected a reduction in beta-spectrin protein levels, and a significant increase in transmembranous alpha 3 (catalytic) subunit of the Na+, K+-ATPase in alcoholic subjects. However, control subjects retained stable oligomeric forms of a-subunit that were diminished in alcoholics. In alcoholics, significant loss of cytosolic alpha-and beta-tubulins were also seen in caudate nucleus, hippocampus and cerebellum, but to different levels, indicative of brain regional susceptibility to alcohol-related damage. Collectively, these protein changes provide a molecular basis for some of the neuronal and behavioural abnormalities attributed to alcoholics

Transverse target single-spin asymmetry in inclusive electroproduction of charged pions and kaons

Single-spin asymmetries were investigated in inclusive electroproduction of charged pions and kaons from transversely polarized protons at the HERMES experiment. The asymmetries were studied as a function of the azimuthal angle psi about the beam direction between the target-spin direction and the hadron production plane, the transverse hadron momentum P-T relative to the direction of the incident beam, and the Feynman variable x(F). The sin psi* amplitudes are positive for pi(+) and K+ slightly negative for pi(-) and consistent with zero for K-, with particular P-T but weak x(F) dependences. Especially large asymmetries are observed for two small subsamples of events, where also the scattered electron was recorded by the spectrometer. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved.

Detection of particles with a cloud chamber

Cloud chambers were essential devices in early nuclear and particle physics research. Superseded by more modern detectors in actual research, they still remain very interesting pedagogical apparatus. This thesis attempts to give a global view on this topic. To do so, a review of the physical foundations of the diffusion cloud chamber, in which an alcohol is supersaturated by cooling it with a thermal reservoir, is carried out. Its main results are then applied to analyse the working conditions inside the chamber. The analysis remarks the importance of using an appropriate alcohol, such as isopropanol, as well as a strong cooling system, which for isopropanol needs to reach −40ºC. That theoretical study is complemented with experimental tests that were performed with what is the usual design of a home-made cloud chamber. An effective setup is established, which highlights details such as a grazing illumination, a direct contact with the cooling reservoir through a wide metal plate, or the importance of avoiding vapour removal. Apart from that, video results of different phenomena that cloud chamber allow to observe are also presented. Overall, it is aimed to present a physical insight that pedagogical papers usually lack.

Recent HERMES results from inclusive and semi-inclusive hadron production

4th International Workshop on Transverse Polisarization Phenomena in Hard Processes (TRANSVERSITY 2014)