10 resultados para 323-U1339B
em Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco
Resumo:
Eguíluz, Federico; Merino, Raquel; Olsen, Vickie; Pajares, Eterio; Santamaría, José Miguel (eds.)
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Santamaría, José Miguel; Pajares, Eterio; Olsen, Vickie; Merino, Raquel; Eguíluz, Federico (eds.)
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Raquel Merino Álvarez, José Miguel Santamaría, Eterio Pajares (eds.)
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Introduction: Acinetobacter baumannii is opportunistic in debilitated hospitalised patients. Because information from some South American countries was previously lacking, this study examined the emergence of multi-resistant A. baumannii in three hospitals in Cochabamba, Bolivia, from 2008 to 2009. Methodology: Multiplex PCR was used to identify the main resistance genes in 15 multi-resistant A. baumannii isolates. RT-PCR was used to measure gene expression. The genetic environment of these genes was also analysed by PCR amplification and sequencing. Minimum inhibitory concentrations were determined for key antibiotics and some were determined in the presence of an efflux pump inhibitor, 1-(1-napthylmethyl) piperazine. Results: Fourteen strains were found to be multi-resistant. Each strain was found to have the bla(OXA-58) gene with the ISAba3-like element upstream, responsible for over-expression of the latter and subsequent carbapenem resistance. Similarly, ISAba1, upstream of the bla(ADC) gene caused over-expression of the latter and cephalosporin resistance; mutations in the gyrA(Ser83 to Leu) and parC (Ser-80 to Phe) genes were commensurate with fluoroquinolone resistance. In addition, the adeA, adeB efflux genes were over-expressed. All 15 isolates were positive for at least two aminoglycoside resistance genes. Conclusion: This is one of the first reports analyzing the multi-drug resistance profile of A. baumannii strains isolated in Bolivia and shows that the over-expression of thebla(OXA-58), bla(ADC) and efflux genes together with aminoglycoside modifying enzymes and mutations in DNA topoisomerases are responsible for the multi-resistance of the bacteria and the subsequent difficulty in treating infections caused by them.
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11p. -- [Nota: Variante del título de la publicación: Boletín electrónico de Hegoa]
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[ES] En el siguiente trabajo se dan a conocer cuatro nuevas estelas funerarias de época romana de Belorado (Burgos). Como la inmensa mayoría de las estelas localizadas en este lugar, estas también guardan una serie de peculiaridades externas e internas comunes que han llevado a considerarlo un conjunto epigráfico específico del lugar. Es por ello que en las siguientes páginas presentaremos una descripción y análisis de las mismas a la vez que las contextualizamos dentro del conjunto y el lugar donde fueron localizadas.
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323 p.
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[ES] En este artículo presentamos nuestra tercera aportación de novedades de la epigrafía de época romana de la provincia de Segovia, con seis piezas nuevas, recogemos dos ya publicadas, de una aportamos nueva lectura e interpretación, e introducimos un apartado de corrigenda con dos entradas.
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323 p.
Resumo:
323 p.