Modelo de solicitud de documentación sobre la Guerra Civil del 36 elaborado por el Centro Vasco de Caracas.

Fecha: >1970 / Unidad de instalación: Carpeta 48 - Expediente 7-14 / Nº de pág.: 3 (mecanografiadas)

Phosphorylation of the carboxy-terminal domain of histone H1: effects on secondary structure and DNA condensation

Linker histone H1 plays an important role in chromatin folding. Phosphorylation by cyclin-dependent kinases is the main post-translational modification of histone H1. We studied the effects of phosphorylation on the secondary structure of the DNA-bound H1 carboxy-terminal domain (CTD), which contains most of the phosphorylation sites of the molecule. The effects of phosphorylation on the secondary structure of the DNA-bound CTD were site-specific and depended on the number of phosphate groups. Full phosphorylation significantly increased the proportion of -structure and decreased that of -helix. Partial phosphorylation increased the amount of undefined structure and decreased that of -helix without a significant increase in -structure. Phosphorylation had a moderate effect on the affinity of the CTD for the DNA, which was proportional to the number of phosphate groups. Partial phosphorylation drastically reduced the aggregation of DNA fragments by the CTD, but full phosphorylation restored to a large extent the aggregation capacity of the unphosphorylated domain. These results support the involvement of H1 hyperphosphorylation in metaphase chromatin condensation and of H1 partial phosphorylation in interphase chromatin relaxation. More generally, our results suggest that the effects of phosphorylation are mediated by specific structural changes and are not simply a consequence of the net charge.

Aplicación de la norma internacional de contabilidad nic 36 deterioro de valor de los activos en entidades cotizadas

Idioma del TFG: castellano. Análisis de lo que implica la aplicación de la norma internacional de contabilidad y realizar una breve comparativa con la normativa española, destacar si existen diferentes, y observar y analizar como afecta y se implanta en empresas pertenecientes al IBEX 35, tanto del mismo sector como sectores distintos.