rhegf-Loaded Plga-Alginate Microspheres Enhance The Healing Of Full-Thickness Excisional Wounds In Diabetised Wistar Rats

[EN] Diabetic foot ulcers (DFUs) represent a major clinical challenge in the ageing population. To address this problem, rhEGF-loaded Poly-Lactic-co-Glycolic-Acid (PLGA)-Alginate microspheres (MS) were prepared by a modified w/o/w-doubleemulsion/ solvent evaporation method. Different formulations were evaluated with the aim of optimising MSs properties by adding NaCl to the surfactant solution and/or the solvent removal phase and adding alginate as a second polymer. The characterization of the developed MS showed that alginate incorporation increased the encapsulation efficiency (EE) and NaCl besides increasing the EE also became the particle surface smooth and regular. Once the MS were optimised, the target loading of rhEGF was increased to 1% (PLGA-Alginate MS), and particles were sterilised by gamma radiation to provide the correct dosage for in vivo studies. In vitro cell culture assays demonstrated that neither the microencapsulation nor the sterilisation process affected rhEGF bioactivity or rhEGF wound contraction. Finally, the MS were evaluated in vivo for treatment of the full-thickness wound model in diabetised Wistar rats. rhEGF MS treated animals showed a statistically significant decrease of the wound area by days 7 and 11, a complete re-epithelisation by day 11 and an earlier resolution of the inflammatory process. Overall, these findings demonstrate the promising potential of rhEGF-loaded MS (PLGA-Alginate MS) to promote faster and more effective wound healing, and suggest its possible application in DFU treatment.

More than one language in the brain

Boeckx C., M.C. Horno & J.L. Mendívil (Eds.)

Simple Coalitional Strategy Profiles in Repeated Games

This paper is a version of the discussion paper titled "Simple coalitional strategy profiles"

Análisis de una metodología para el estudio de los textos del periodismo de investigación.

493 p.

TickTASKER, un nuevo proyecto empresarial en el entorno TIC

Business Plan Tick TASKER

Cartas enviadas por José María Bengoa a Justo Garate entre 1940 y 1945

10 cartas (mecanografiadas); entre 210x255mm y 210x310mm. [La carta fechada el 10-11-1942 esta incompleta, falta la primera hoja]

Cartas enviadas por José María Bengoa a Justo Garate entre 1947 y 1961

11 cartas (mecanografiadas y manuscritas); entre 170x225mm y 215x275mm

Cosecha de microalgas con nanopartículas magnéticas

El objetivo de este trabajo es sintetizar nanopartículas de magnetita con grupos hidroxilo y con grupos amino, y estudiar el uso de ambas en la cosecha de dos especies distintas de microalgas creciendo con distintas fuentes de nitrógeno.

Erdi Aroko epidotadun zeramikak egiteko erabilitako buztinen jatorria (Gorlizeko Inmaculada Concepcion Parrokia aztarnategia)

Aurkitutako epidotadun zeramikak Gorliz muinoko iparraldea okupatu duten indibiduoek aztarnategi inguruko sedimentuetatik abiatuz sortutakoak diren ala kanpotik ekarriak diren jakin nahi da. Hala, zeramika hauen jatorria aztertzekorako orduan, inguruko sedimentuen konposizioa ikertuko da epidota dagoen ala ez jakiteko. X izpien difrakzioaren bidez ikusi da epidotaren ebidentzia dagoela mineral astunetan, eta buztin mineralen frakzioan eta lagin osoan epidota aurkitu da. Kokapen geologikora jo ez gero, epidotak Trias Keuper-arekin mozten duten ibaietan aurkitzen direla ikus daiteke. Epidotaren agerpenak adierazten du Gorlizko aztarnategian aurkitutako zeramika arkeologikoak bertakoak direla, ez direla inportatutakoak.

Cartas enviadas por José María Bengoa a Justo Garate entre 1962 y 1992

8 cartas (mecanografiadas y manuscritas); entre 150x210mm y 215x275mm .- 1 Felicitación de Navidad (manuscrita y sin fecha) ; 110mmx160mm

Propiedades catalíticas de la lipasa B de Candida antarctica inmovilizada en soportes magnéticos. Actividad hidrolítica en medios acuosos

Los enzimas son piezas fundamentales en el correcto funcionamiento de cualquier sistema biológico. Gracias a su naturaleza proteica y a las estructuras tridimensionales complejas que son capaces de adoptar, estas moléculas actúan como catalizadores de reacciones químicas. L a función de los enz imas es disminuir la energía de activación de la reacción, aumentando de este modo la velocidad de reacción. L o s enzimas no alteran el balance e nergético de las reacciones en que intervienen, ni modifican, por lo tanto, el equilibrio de la reacción . Por este motivo, en las reacciones catalizadas por enzimas se observa una mayor rapidez a la hora de alcanzar el equilibrio. La ciencia que estudia l a velocidad de las reacciones químicas que son catalizadas por enzimas es la cinética enzimática , e n la cual , las moléculas sobre las que actúan los enzimas se denominan sustratos y las moléculas resultantes de la conversión productos. El estudio de la cin ética de un enzima permite explicar los detalles de su mecanismo catalítico, su papel en el metabolismo o incluso cómo se controla su actividad en la célula. Las dos propiedades más importantes a la hora de trabajar con enzimas son: el tiempo que tarda en saturarse con un sustrato en particular y la velocidad máxima de reacción que puede alcanzar. Para el estudio de estas propiedades en el laboratorio se realizan los ensayos enzimáticos. El procedimiento a seguir en estos casos es medir la aparición de un producto o la desaparición de un sustrato frente al tiempo.

Cartas enviadas por María Milagros Bidegain a Justo Garate entre 1971 y 1972

2 cartas (mecanografiada y manuscrita); entre 215x155mm y 210x315mm.

Cosméticos María D'uol: Gulliver en el país de los gigantes

Duración (en horas): De 21 a 30 horas Destinatario: Estudiante y Docente

Magnetic Cross-Linked Enzyme Aggregates (mCLEAs) of Candida antarctica Lipase: An Efficient and Stable Biocatalyst for Biodiesel Synthesis

Enzyme-catalyzed production of biodiesel is the object of extensive research due to the global shortage of fossil fuels and increased environmental concerns. Herein we report the preparation and main characteristics of a novel biocatalyst consisting of Cross-Linked Enzyme Aggregates (CLEAs) of Candida antarctica lipase B (CALB) which are covalently bound to magnetic nanoparticles, and tackle its use for the synthesis of biodiesel from non-edible vegetable and waste frying oils. For this purpose, insolubilized CALB was covalently cross-linked to magnetic nanoparticles of magnetite which the surface was functionalized with –NH2 groups. The resulting biocatalyst combines the relevant catalytic properties of CLEAs (as great stability and feasibility for their reutilization) and the magnetic character, and thus the final product (mCLEAs) are superparamagnetic particles of a robust catalyst which is more stable than the free enzyme, easily recoverable from the reaction medium and reusable for new catalytic cycles. We have studied the main properties of this biocatalyst and we have assessed its utility to catalyze transesterification reactions to obtain biodiesel from non-edible vegetable oils including unrefined soybean, jatropha and cameline, as well as waste frying oil. Using 1% mCLEAs (w/w of oil) conversions near 80% were routinely obtained at 30°C after 24 h of reaction, this value rising to 92% after 72 h. Moreover, the magnetic biocatalyst can be easily recovered from the reaction mixture and reused for at least ten consecutive cycles of 24 h without apparent loss of activity. The obtained results suggest that mCLEAs prepared from CALB can become a powerful biocatalyst for application at industrial scale with better performance than those currently available.

Efficacy and safety of autologous platelet rich plasma for the treatment of vascular ulcers in primary care: Phase III study

Background: Vascular ulcers are commonly seen in daily practice at all levels of care and have great impact at personal, professional and social levels with a high cost in terms of human and material resources. Given that the application of autologous platelet rich plasma has been shown to decrease healing times in various different studies in the hospital setting, we considered that it would be interesting to assess the efficacy and feasibility of this treatment in primary care. The objectives of this study are to assess the potential efficacy and safety of autologous platelet rich plasma for the treatment of venous ulcers compared to the conventional treatment (moist wound care) in primary care patients with chronic venous insufficiency (C, clinical class, E, aetiology, A, anatomy and P, pathophysiology classification C6). Design: We will conduct a phase III, open-label, parallel-group, multicentre, randomized study. The subjects will be 150 patients aged between 40 and 100 years of age with an at least 2-month history of a vascular venous ulcer assigned to ten primary care centres. For the treatment with autologous platelet rich plasma, all the following tasks will be performed in the primary care setting: blood collection, centrifugation, separation of platelet rich plasma, activation of coagulation adding calcium chloride and application of the PRP topically after gelification. The control group will receive standard moist wound care. The outcome variables to be measured at baseline, and at weeks 5 and 9 later include: reduction in the ulcer area, Chronic Venous Insufficiency Quality of Life Questionnaire score, and percentage of patients who require wound care only once a week. Discussion: The results of this study will be useful to improve the protocol for using platelet rich plasma in chronic vascular ulcers and to favour wider use of this treatment in primary care.