Wide Screening of Phage-Displayed Libraries Identifies Immune Targets in Planta

Microbe-Associated Molecular Patterns and virulence effectors are recognized by plants as a first step to mount a defence response against potential pathogens. This recognition involves a large family of extracellular membrane receptors and other immune proteins located in different sub-cellular compartments. We have used phage-display technology to express and select for Arabidopsis proteins able to bind bacterial pathogens. To rapidly identify microbe-bound phage, we developed a monitoring method based on microarrays. This combined strategy allowed for a genome-wide screening of plant proteins involved in pathogen perception. Two phage libraries for high-throughput selection were constructed from cDNA of plants infected with Pseudomonas aeruginosa PA14, or from combined samples of the virulent isolate DC3000 of Pseudomonas syringae pv. tomato and its avirulent variant avrRpt2. These three pathosystems represent different degrees in the specificity of plant-microbe interactions. Libraries cover up to 26107 different plant transcripts that can be displayed as functional proteins on the surface of T7 bacteriophage. A number of these were selected in a bio-panning assay for binding to Pseudomonas cells. Among the selected clones we isolated the ethylene response factor ATERF-1, which was able to bind the three bacterial strains in competition assays. ATERF-1 was rapidly exported from the nucleus upon infiltration of either alive or heat-killed Pseudomonas. Moreover, aterf-1 mutants exhibited enhanced susceptibility to infection. These findings suggest that ATERF-1 contains a microbe-recognition domain with a role in plant defence. To identify other putative pathogen-binding proteins on a genome-wide scale, the copy number of selected-vs.-total clones was compared by hybridizing phage cDNAs with Arabidopsis microarrays. Microarray analysis revealed a set of 472 candidates with significant fold change. Within this set defence-related genes, including well-known targets of bacterial effectors, are over-represented. Other genes non-previously related to defence can be associated through this study with general or strain-specific recognition of Pseudomonas.

La pervivencia de la literatura anglosajona: reescritura, traducción, creación

Eterio Pajares, Raquel Merino y José Miguel Santamaría (eds.)

Adaptación cinematográfica y traducción: en busca de normas de transferencia en el ámbito hispano-alemán

Raquel Merino Álvarez, José Miguel Santamaría, Eterio Pajares (eds.)

Análisis de las publicaciones del personal docente e investigador de las facultades españolas de Bellas Artes

8 p.

Valor social y ética del deporte

Ponencia invitado en el congreso internacional XII congreso AEISAD."El Factor Humano: Cuando la diferencia la marcan las personas" 25/10/2012 San Sebastián - Coeditado por: Juan Aldaz Arregui, Alberto Dorado Suárez, Pedro Jesús Jiménez Martín, Anna Vilanova Soler

Grafika Teknologikoa

Duración (en horas): Más de 50 horas. Destinatario: Estudiante y Docente

Desarrollo de las actividades atléticas en la vida cotidiana

Duración (en horas): Más de 50 horas. Destinatario: Estudiante y Docente

Biocompatible Bacterial Cellulose-Poly(2-hydroxyethyl methacrylate) Nanocomposite Films

A series of bacterial cellulose-poly(2-hydroxyethyl methacrylate) nanocomposite films was prepared by in situ radical polymerization of 2-hydroxyethyl methacrylate (HEMA), using variable amounts of poly(ethylene glycol) diacrylate (PEGDA) as crosslinker. Thin films were obtained, and their physical, chemical, thermal, and mechanical properties were evaluated. The films showed improved translucency compared to BC and enhanced thermal stability and mechanical performance when compared to poly(2-hydroxyethyl methacrylate) (PHEMA). Finally, BC/PHEMA nanocomposites proved to be nontoxic to human adipose-derived mesenchymal stem cells (ADSCs) and thus are pointed as potential dry dressings for biomedical applications.

The Expanded mtDNA Phylogeny of the Franco-Cantabrian Region Upholds the Pre-Neolithic Genetic Substrate of Basques

9 p.

The IRYSS-COPD appropriateness study: objectives, methodology, and description of the prospective cohort

Background: Patients with chronic obstructive pulmonary disease (COPD) often experience exacerbations of the disease that require hospitalization. Current guidelines offer little guidance for identifying patients whose clinical situation is appropriate for admission to the hospital, and properly developed and validated severity scores for COPD exacerbations are lacking. To address these important gaps in clinical care, we created the IRYSS-COPD Appropriateness Study. Methods/Design: The RAND/UCLA Appropriateness Methodology was used to identify appropriate and inappropriate scenarios for hospital admission for patients experiencing COPD exacerbations. These scenarios were then applied to a prospective cohort of patients attending the emergency departments (ED) of 16 participating hospitals. Information was recorded during the time the patient was evaluated in the ED, at the time a decision was made to admit the patient to the hospital or discharge home, and during follow-up after admission or discharge home. While complete data were generally available at the time of ED admission, data were often missing at the time of decision making. Predefined assumptions were used to impute much of the missing data. Discussion: The IRYSS-COPD Appropriateness Study will validate the appropriateness criteria developed by the RAND/UCLA Appropriateness Methodology and thus better delineate the requirements for admission or discharge of patients experiencing exacerbations of COPD. The study will also provide a better understanding of the determinants of outcomes of COPD exacerbations, and evaluate the equity and variability in access and outcomes in these patients.

Introducción a la economía de la empresa

1.Alumnos de primer curso de la Licenciatura de Administración de Empresas. 2.Alumnos de la Licenciatura de Economía que quieran acercarse al estudio de temas relacionados con la administración de Empresas. 3.Cualquier persona que se acerque por primera vez al estudio de aspectos relacionados con la administración- gestión empresarial. El presente material recoge los siguientes aspectos: 1.Programa de la asignatura. 2.Planteamiento de las competencias que pueden ser adquiridas con la ayuda del material que se presenta. 3.Planteamiento de objetivos particulares para cada una de las partes en la que se ha divido el material, que se corresponde con las diferentes áreas funcionales de la empresa. 4.Desarrollo de cada uno de los temas que figuran en el programa. 5.Bibliografía utilizada para la elaboración del material y que puede ser utilizada en caso de que las personas que utilicen el material quieran profundizar en alguno de los temas que se presenta.

Impacto del ejercicio físico sobre la calidad de vida en las personas que padecen cardiopatía isquémica

184 p.

Estudio de la Formaci??n de Profesores para el Desarrollo de la Equitaci??n y la Discapacidad

El proyecto sobre el tipo de formaci??n que deben de tener los profesionales que se dedican a trabajar en la equitaci??n con personas discapacitadas. En castellano.