23 resultados para Agolia, Rodolfo Mario


Relevância:

10.00% 10.00%

Publicador:

Resumo:

Nucleophosmin (NPM) is a nucleocytoplasmic shuttling protein, normally enriched in nucleoli, that performs several activities related to cell growth. NPM mutations are characteristic of a subtype of acute myeloid leukemia (AML), where mutant NPM seems to play an oncogenic role. AML-associated NPM mutants exhibit altered subcellular traffic, being aberrantly located in the cytoplasm of leukoblasts. Exacerbated export of AML variants of NPM is mediated by the nuclear export receptor CRM1, and due, in part, to a mutationally acquired novel nuclear export signal (NES). To gain insight on the molecular basis of NPM transport in physiological and pathological conditions, we have evaluated the export efficiency of NPM in cells, and present new data indicating that, in normal conditions, wild type NPM is weakly exported by CRM1. On the other hand, we have found that AML-associated NPM mutants efficiently form complexes with CRM1HA (a mutant CRM1 with higher affinity for NESs), and we have quantitatively analyzed CRM1HA interaction with the NES motifs of these mutants, using fluorescence anisotropy and isothermal titration calorimetry. We have observed that the affinity of CRM1HA for these NESs is similar, which may help to explain the transport properties of the mutants. We also describe NPM recognition by the import machinery. Our combined cellular and biophysical studies shed further light on the determinants of NPM traffic, and how it is dramatically altered by AML-related mutations.

Relevância:

10.00% 10.00%

Publicador:

Resumo:

La seguridad urbana y el miedo al delito son unos de los temas que actualmente más preocupan no sólo a los gestores de las ciudades, sino también a los propios ciudadanos ya que afecta de manera clara a la calidad de vida. Por todo ello se realizó este estudio, a través de una encuesta de 106 personas en la ciudad de Donostia-San Sebastián, para conocer la situación de la seguridad tanto objetiva como subjetiva de los habitantes de la ciudad.

Relevância:

10.00% 10.00%

Publicador:

Resumo:

166 p. : il. col.

Relevância:

10.00% 10.00%

Publicador:

Resumo:

Nivel educativo: Grado. Duración (en horas): De 41 a 50 horas

Relevância:

10.00% 10.00%

Publicador:

Resumo:

Nivel educativo: Grado. Duración (en horas): Más de 50 horas

Relevância:

10.00% 10.00%

Publicador:

Resumo:

Nivel educativo: Grado. Duración (en horas): Más de 50 horas

Relevância:

10.00% 10.00%

Publicador:

Resumo:

Nivel educativo: Grado. Duración (en horas): De 41 a 50 horas

Relevância:

10.00% 10.00%

Publicador:

Resumo:

[eus] Gizarte mugimenduen teorien inguruko hurbilpen bat dugu lan hau. Ikuspegi klasikoak aztertzeaz gain, azken hamarkadetan agertu diren bestelako gaiak eta perspektibak kontutan hartzen dituena. Lan honen bitartez perspektiba hauek proposatzen duten bidea aztertu dugu, azken batean eta epe luzera gizarte mugimenduen inguruko teoria oso bat garatzeko lehenengo pausuak emateko helburuarekin.