Di and tripeptides from marine sources can target adipogenic process and contribute to decrease adipocyte number and functions

The effect of 11 marine-derived cryptides was investigated on proliferation, differentiation and maturation of human white pre-adipocytes (HWP). They were all formerly identified as potent Angiotensin-Converting-Enzyme inhibitors.Val-Trp (VW),Val-Tyr (VY), Lys-Tyr (KY), Lys-Trp (KW), Ile-Tyr (IY), Ala-Pro (AP),Val-Ile-Tyr (VIY), Leu-Lys-Pro (LKP), Gly-Pro-Leu (GPL), Ala-Lys-Lys (AKK) and Val-Ala-Pro (VAP) were previously found in fish products and coproducts as well as other marine resources like wakame. Treatment with AP, VAP and AKK greatly affected viability of HWP during the proliferation period while KW and VW treatment reduced the number of viable cells during the differentiation stage. A GPL and IY incubation during the differentiation stage allowed the decrease of their final lipid content, of the GPDH activity and of the mRNA level of adipocyte markers (aP2, GLUT4, LPL and AGT). Moreover, a down regulation of both PPARγ and C/EBPα expression, two key regulators of adipogenesis,was observed.These findings indicate that small bioactive peptides from marine protein hydrolysates can target adipogenesis and thus could regulate energy metabolism disorders

Crassaminicella profunda gen. nov., sp nov., an anaerobic marine bacterium isolated from deep-sea sediments

A novel, anaerobic, chemo-organotrophic bacterium, designated strain Ra1766HT, was isolated from sediments of the Guaymas basin (Gulf of California, Mexico) taken from a depth of 2002 m. Cells were thin, motile, Gram-stain-positive, flexible rods forming terminal endospores. Strain Ra1766H(T) grew at temperatures of 25-45 degrees C (optimum 30 degrees C), pH 6.7-8.1 (optimum 7.5) and in a salinity of 5-60 g l(-1) NaCl (optimum 30 g l(-1)). It was an obligate heterotrophic bacterium fermenting carbohydrates (glucose and mannose) and organic acids (pyruvate and succinate). Casamino acids and amino acids (glutamate, aspartate and glycine) were also fermented. The main end products from glucose fermentation were acetate, butyrate, ethanol, H-2 and CO2. Sulfate, sulfite, thiosulfate, elemental sulfur, fumarate, nitrate, nitrite and Fe(III) were not used as terminal electron acceptors. The predominant cellular fatty acids were C-14 : 0, C-16:1 omega 7, C-16:1 omega 7 DMA and C-16:0. The main polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phospholipids. The G +C content of the genomic DNA was 33.7 molo/o. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain Ra1766H(T) was affiliated to cluster XI of the order Clostridia les, phylum Firmicutes. The closest phylogenetic relative of Ra1766H(T) was Geosporobacter subterraneus (94.2% 16S rRNA gene sequence similarity). On the basis of phylogenetic inference and phenotypic properties, strain Ra1766H(T) (=DSM 27501(T)=JCM 19377(T)) is proposed to be the type strain of a novel species of a novel genus, named Crassaminicella pro funda.

Copernicus. Product User Manual In Situ TAC products

This guide describes the data product files from the Copernicus Marine Environment (CMEMS) In Situ Thematic Centre, what data services are available to access them, and how to use the files and services.

Stimulation of bacterial exoproteolytic activity by fish farming in coastal marine ponds: Effect on dissolved protein cycling

The effect of fish farming on dissolved amino acid concentrations, bacterioplankton abundance and exoproteolytic activity was assessed in 3 experimental marine ponds. Different standing stocks of fish were introduced (semi-intensive pond: 250 g.m(-2); semi-extensive pond: 50 g.m(-2) control pond: 0). Sea bass farming increased dissolved combined amino acid (DCAA) concentrations only in the semi-intensive pond. Bacterial standing stock was unaffected by fish food supply. However, bacterial exoproteolytic activity was strongly stimulated by aquaculture intensification; the average maximal rate of dissolved protein hydrolysis (V-m) increased with intensity (control pond: 1 500 nM.h(-1); semi-extensive pond: 2 600 nM.h(-1) semi-intensive pond: 5 100 nM.h(-1)). DCAA fluxes through bacterial exoproteolytic activity ranged between 16 (semi-extensive) and 11% (semi-intensive) of the daily nitrogen input by fish food. Bacterial exoproteolytic activity allowed a substantial part of the increased supply of dissolved amino nitrogen to be incorporated into bacterial biomass, then available for transfer to higher trophic levels within the ponds. It also significantly decreased dissolved organic nitrogen export from the ponds to the surrounding environment.

IQuOD 4th workshop in Tokyo: Copernicus Marine in situ TAC, a service for operational oceanography

Copernicus is a European system for monitoring the Earth. COPERNICUS-CMEMS products and services are meant to serve all marine applications: Marine resources, Maritime safety, Coastal and Marine Environment, Seasonal Forecast & Climate. The service is ambitious as the ocean is complex and many processes are involved, from physical oceanography, biology, geology, ocean-atmosphere fluxes, solar radiations, moon induced tides, anthropic activity. A multi-platform approach is essential, taking into account sea-level stations, coastal buoys, HF radars, river flows, drifting buoys, sea-mammal or fishes fitted with sensors, vessels, gliders, floats.

Sextant. A spatial data infrastructure for marine environments

At national and European levels, in various projects, data products are developed to provide end-users and stakeholders with homogeneously qualified observation compilation or analysis. Ifremer has developed a spatial data infrastructure for marine environment, called Sextant, in order to manage, share and retrieve these products for its partners and the general public. Thanks to the OGC and ISO standard and INSPIRE compliance, the infrastructure provides a unique framework to federate homogeneous descriptions and access to marine data products processed in various contexts, at national level or European level for DG research (SeaDataNet), DG Mare (EMODNET) and DG Growth (Copernicus MEMS). The discovery service of Sextant is based on the metadata catalogue. The data description is normalized according to ISO 191XX series standards and Inspire recommendations. Access to the catalogue is provided by the standard OGC service, Catalogue Service for the Web (CSW 2.0.2). Data visualization and data downloading are available through standard OGC services, Web Map Services (WMS) and Web Feature Services (WFS). Several OGC services are provided within Sextant, according to marine themes, regions and projects. Depending on the file format, WMTS services are used for large images, such as hyperspectral images, or NcWMS services for gridded data, such as climatology models. New functions are developped to improve the visualization, analyse and access to data, eg : data filtering, online spatial processing with WPS services and acces to sensor data with SOS services.