Mean structure of the North Atlantic subtropical permanent pycnocline from in-situ observations

In the north Atlantic subtropical gyre, the oceanic vertical structure of density is characterized by a region of rapid increase with depth. This layer is called the permanent pycnocline. The permanent pycnocline is found below a surface mode water ,which are ventilated every winter when penetrated locally by the mixed layer. Assessing the structure and variability of the permanent pycnocline is of a major interest in the understanding of the climate system because the pycnocline layer delimits important heat and anthropogenic reservoir. Moreover, the heat content structure translate into changes in the large scale stratification feature, such as the permanent pycnocline. We developed a new objective algorithm for the characterization of the large scale structure of the permanent pycnocline (OAC-P). Argo data have been used with OAC-P to provide a detailed description of the mean structure of the North-Atlantic subtropical pycnocline (e.g.: depth, thickness, temperature, salinity, density, potential vorticity). Results reveal a surprisingly complex structure with inhomogeneous properties. While the classical bowl shape of the pycnocline depth is captured, much more complex pycnocline structure emerges at the regional scale. In the southern recirculation gyre of the Gulf Stream Extension, the pycnocline is deep, thick, the maximum of stratification is found in the middle on the layer and follow an isopycnal surface. But local processes influence and modify this textbook description and the pycnocline is characterized by a vertically asymmetric structure and gradients in thermohaline properties. T/S distribution along the permanent pycnocline depth is complex and reveals a diversity of water masses resulting from mixing of different source waters. We will present the observed mean structure of the North-Atlantic subtropical permanent pycnocline and relate it to physical processes that constraint it.

Bacterial community structure of the marine diatom Haslea ostrearia

The marine diatom Haslea ostrearia produces a water-soluble blue-pigment named marennine of economic interest (e.g. in aquaculture for the greening of oysters). Up to date the studies devoted to ecological conditions under which this microalga develops never took into account the bacterial-H. ostrearia relationships. In this study the bacterial community was analysed by PCR-TTGE before and after H. ostrearia isolation cells recovered from 4 localities, to distinguish the relative part of the biotope and the biocenose and eventually to describe the temporal dynamic of the structure of the bacterial community. The bacterial structure of the phycosphere differed strongly from that of the bulk sediment. The similarity between bacteria recovered from the biofilm and the suspended bacteria did not exceed 10% (vs. > 90% amongst biofilms). The differences in genetic fingerprints, more especially high between two H. ostrearia isolates showed also the highest differences in the bacterial structure as the result of specific metabolomics profiles. The non-targeted metabolomic investigation showed that these profiles were more distinct in case of bacteria-alga associations than for the H. ostrearia monoculture. At the scale of a culture cycle in laboratory conditions, the bacterial community was specific to the growth stage. When H. ostrearia was subcultured for 9 months, a shift in the bacterial structure was shown from 3-months subculturing and the bacterial structure stabilized afterwards (70-86% similarities). A first insight of the relationships between H. ostrearia and its surrounding bacteria was shown for a better understanding of the ecological feature of this diatom.