Refolding of omega conotoxin peptide derived from marine snail, Conus magus, to evaluate its analgesic effects

Oxidative refolding is one of the key challenges hampering the development of peptide based compounds as therapeutics. The correct refolding for three disulfide peptide like w-Conotoxi n MVIIA is difficult and crucial for biological activity. This work advanced knowledge of chemical and biological for improve oxidative refolding of synthetic w-Conotoxi n MVIIA in base of Conus magus venom. The present study aimed to set up an appropriate and effective protocols for refolding of disulfide-rich w-Conotoxin MVIIA. In this study, the crude peptide was protected with Acm group, according to the right amino acid sequences (Synthesized by Australian Company). The crude peptide was purified by H PLC. To prepare the peptide to refolding, innovative deprotection applied molar ratio (AMR) method was performed based on mercury. Accuracy of deprotection was approved by reverse phase chromatography. The deprotected target peptide (omega-conotoxin) was determined by SDS-PAGE. Then the Oxidative refolding of target peptide was performed in six protocol based on Guanidinium chloride and oxidized and reduced Glutathione. Analgesic effect of refolded peptide was surveyed with formalin test in mice Balb/c. Non neurotoxic effects of target peptides were survey with ICV injection in mice model (C57/BL6). The innovative deprotection protocol performed based on the best ratio of mercury/2-mercaptoethanol adjusted to 1mg/10p1 in 90 minute. The results showed the yield and purity of omega-conotoxin MVIIA as 93 and 95%, respectively. Refolding of 40 mg omega Conotoxin with GSSG and GSH on ratio of 10:1 and 20 mM ammonium acetate showed the best analgesic effect compared with the other methods. The result showed 95.5% yield and 98% purity of omega-conotoxin MVIIA in this refolding method. Related refolding method reduced 85% pain in experimented mice using 7 ng of the peptide. That was 71.5 fold stronger than morphine and 2 times than standard Prialt®. And it was not neurotoxic in mice. In this study, refolding method for omega-conotoxin MVIIA was optimized in the fourth factor including: reducing the time, amount and number of reagent and increase the efficiency. We introduced new method for deprotection of omega-conotoxin MVIIA. Effective, economic and applied refolding and deprotecti on method was performed in this research may al so be applied to similar omega conotoxin peptides.

A biologically active peptide in the skin of lampreys (Eudontomyzon danfordi vladykovi). [Translation from: Z.Naturforsch. (B), 26(10), 1021-1023, 1971. ]

In recent years interest in the production and description of kinin-type substances has been greatly intensified. So, for example, bradykinin, phyllokinin, physalaemin, ranatensin and caerulein could be extracted from the skin of amphibians as well as. eledoisin out of the salivary glands of Eledon moschata. An examination of lampreys seemed to us particularly profitable in the search for the incidence of further kinins. Ammocoetes of different sizes and also adults of both sexes of the species Eudontomyzon danfordi vladykovi were studied in this research. This species is found in many tributaries of the Danube. Skin extracts were tested on on isolated rat uterus, rat duodenum, guinea pig ileum and rabbit jejunum, further tests were done in order to determine a peptide character of the biologically active substance.

Drought frequency analysis for California from observed, synthetic, and proxy data

Drought frequency analysis can be performed with statistical techniques developed for determining recurrence intervals for extreme precipitation and flood events (Linsley et al 1992). The drought analysis method discussed in this paper uses the log-Pearson Type III distribution, which has been widely used in flood frequency research. Some of the difficulties encountered when using this distribution for drought analysis are investigated.

The protein, peptide and free amino-acid contents of some species of Gracilaria from south-east coast of India

Four species of Gracilaria are investigated for their free amino-acid contents, as well as amino-acid constituents in the proteins and the peptides, using quantitative paper chromatographic technique. Amino-acid constituents of different species of Gracilaria differ only in amount, while free amino-acids and the amino-acids in the peptides vary both in quality and quantity. A number of amino-acids recorded as protein constituents have even escaped detection in the peptides, while in the free state they occur either in all the species or in some only except homocystine. Moreover, some amino-acids occur exclusively in the free state.

Culture feasibility of african catfish (Clarias gariepinus Lin.) fry in glass tank and synthetic hapa system using supplemental diets

An experiment was carried out with 1 0 days old Clarias gariepinus fry over a period of 42 days to determine the effects of different feeds on growth and survival of African catfish fry in glass tanks. The experiment was designed into four treatments each having three replications. Thus treatment 1 (T1) was named as Tank Tubifex (TT) and treatment 2 (T2) as Tank Sabinco (TS), treatment 3 as Pond Tubifex (PT), and treatment 4 (T4) as Pond Sabinco (PS). Live Tubifex (protein levels 64.48%) was supplied to treatments 1 and 3 and rest of the treatments were supplied Sabinco starter-1 (protein levels 40.13%). The highest and the lowest growth in total length and weight were 12.90cm, 18.77g and 6.17cm, 4.04g recorded from the treatments 3 and 2, respectively. Growth of catfish fry under treatment 3 in terms of both length and weight were significantly higher (P<0.01) than those of the other treatments. However treatment 2 showed the significantly lowest (P<0.01) growth performance among the various treatments. The highest survival rate (92%) was also obtained with treatment 3. Tubifex proved to be the best larval feed in respect of growth and survival rate.

Synthetic fibres for fishing gear

A variety of synthetic fibres are at present in use as fishing gear material, the earliest one being the polyvinyl chloride group introduced for traps in 1936 (von. Brandt 1957). Since then synthetic fibres became increasingly popular among the advanced fishing nations of the world. The latest synthetic fibre which has been successfully used in fishing is polypropylene developed in Italy in 1954.