Recent advances in the hatchery production of seed of some commercial bivalves in India

The Central Marine Fisheries Research Institute has established a shellfish hatchery laboratory at its Tuticorin Research Centre with the objective of developing appropriate technology for the production of seed of commercial bivalves. A viable technology was developed for the mass production of the seed of Pinctada fucata, P. margaretifera, Crassostrea madrasensis, Perna indica, P. viridis, Anadara granosa and Meretrix meretrix. The hatchery facility and the technology, comprising brood stock conditioning, induced spawning, larval rearing and seed production are described. In the light of these developments the future outlook is appraised.

Livelihood and seed mortality related vulnerability of carp seed traders of Bangladesh

Carp seed traders in Bangladesh are considered as one of the poorest, most vulnerable and least studied sub-population. Millions of fingerlings are transported annually to various parts of the country using bus tops and/or truck-beds in open and hand agitated method by the traders and as a result they encounter mass seed mortality. They are the key players in augmenting aquaculture activities by distributing fish fry and fingerlings to the fish farmers. Assessment of the seed sources and the livelihood of the traders are important to identify their point of entry to the aquaculture sector and further improvement. Status of household asset, income profiles, and risks involved were examined using DFID's sustainable livelihood approach (SLA) framework. Tools used to collect data included group discussions (GD), semi-structured and structured household (HH) survey. Samples (n=637) were randomly drawn purposefully from 10% of the carp seed traders within 11 districts. Sampled HH were classified into six land classes.

Growth response of Heterobranchus longifilis (Valenciennes, 1840) fingerlings fed raw and boiled Mucuna cochinchinensis seed meal

Feeding trial was conducted in static water to assess the growth of H. longifilis fingerlings fed different I inclusion levels of mucuna seed meal (MSM). Raw and boiled MSM were used in the diets at 10%, 20%, 30% and 40% inclusion levels and the performance of fish fed these diets was compared with fish fed a fishmeal-based diet which contained 40% protein. All diets were prepared to be isonitrogenous and isocaloric A two by five factorial experiment with three replicates using ten fish of average initial weight 1 .46g was carried out. Daily fish ration of five percent body weight was administered two times for eight weeks. The specific growth rate in diets 1 (control) and 6(10% boiled MSM) were similar and significantly (P<0.05) higher than the other dietary groups. The significantly lower growth performance of fish fed diets containing higher inclusion levels of both raw and boiled MSM might be due to incomplete elimination of the antinutritional factors present in MSM by boiling. Other methods of processing MSM to improve its nutritive value should be investigated. (7 page document)

Ras Behari’s guide to buying fish seed

Document contains 4 pages.

Seed Longevity of Melaleuca quinquenervia: A Burial Experiment in South Florida

Burial and removal techniques with seed bags were used to examine the viability and longevity of Melaleuca quinquenervia seeds at four field sites representing different soil types and hydrological conditions in South Florida. Seed viability was determined over different burial durations in the soil through a combination of germination tests and 2,3,5-triphenyl- tetrazolium chloride (TTC) treatments. Control seeds kept dry at 25 C in the laboratory maintained same viability of ca. 15% over the 3-year study. In the field, seed viability decreased with increased burial duration.(PDF has 4 pages.)

Mass Energy and Flow in closed ecosystems

The general equations of biomass and energy transfer for an n-species, closed ecosystem are written. It is demonstrated how in "ecological time" the parameters describing the dynamics of biomass transfer are related to the parameters of energy transfer, such as respiration, fixation, and energy content. This relationship is determinate for the straight-chain ecosystem, and a simple example is worked out. The results show how the density dependent terms in population dynamics arise naturally, and how the stable system exhibits a hierarchy in energy per unit biomass. A procedure is proposed for extending the theory to include webbed systems, and the particular difficulties involved in the extension are brought before the scientific community for discussion.

Enhancing Seed Availability For the Hard Clam (Mercenaria mercenaria) Aquaculture Industry By Applying Remote Setting Techniques

The goal of this study was to test a technology that may help ensure a reliable and consistent supply of high quality and inexpensive clam seed to growers, thus fostering an emerging aquaculture industry by eliminating a seed shortage that limits sustainability. The overall objectives were to develop, test and demonstrate technical procedures and determine the financial feasibility of transferring remote setting technology from the Pacific Northwest molluscan shellfish industry to the hard clam aquaculture industry in Florida. (PDF has 44 pages.)

Report on Marine Mammal Unusual Mortality Event UMESE0501Sp: Multispecies Mass Stranding of Pilot Whales (Globicephala macrorhynchus), Minke Whale (Balaenoptera acutorostrata), and Dwarf Sperm Whales (Kogia sima) in North Carolina on 15-16 January 2005

On 15-16 January 2005, three offshore species of cetaceans (33 short-finned pilot whales, Globicephala macrorhynchus, one minke whale, Balaenoptera acutorostrata, and two dwarf sperm whales, Kogia sima) stranded alive on the beaches of North Carolina. The pilot whales stranded near Oregon Inlet, the minke whale in northern North Carolina, and the dwarf sperm whales near Cape Hatteras. Live strandings of three species in one weekend was unique in North Carolina and qualified as an Unusual Mortality Event. Gross necropsies were conducted on 16-17 January 2005 on 27 pilot whales, two dwarf sperm whales, and the minke whale. Samples were collected for clinical pathology, parasitology, gross pathology, histopathology, microbiology and serology. There was variation in the number of animals sampled for each collection type, however, due to carcasses washing off the beach or degradation in carcass condition during the course of the response. Comprehensive histologic examination was conducted on 16 pilot whales, both dwarf sperm whales, and the minke whale. Limited organ or only head tissue suites were obtained from nine pilot whales. Histologic examination of tissues began in February 2005 and concluded in December 2005 when final sampling was concluded. Neither the pilot whales nor dwarf sperm whales were emaciated although none had recently ingested prey in their stomachs. The minke whale was emaciated; it was likely a dependent calf that became separated from the female. Most serum biochemistry abnormalities appear to have resulted from the stranding and indicated deteriorating condition from being on land for an extended period. Three pilot whales had clinical evidence of pre-existing systemic inflammation, which was supported by histopathologic findings. Although gross and histologic lesions involving all organ systems were noted, consistent lesions were not observed across species. Verminous pterygoid sinusitis and healed fishery interactions were seen in pilot whales but neither of these changes were causes of debilitation or death. In three pilot whales and one dwarf sperm whale there was evidence of clinically significant disease in postcranial tissues which led to chronic debilitation. Cardiovascular disease was present in one pilot whale and one dwarf sperm whale; musculoskeletal disease and intra-abdominal granulomas were present in two pilot whales. These lesions were possible, but not definitive, causal factors in the stranding. Remaining lesions were incidental or post-stranding. The minke whale and three of five tested pilot whales had positive morbillivirus titers (≥1:8 with one at >1:256), but there was no histologic evidence of active viral infection. Parasites (nematodes, cestodes, and trematodes) were collected from 26 pilot whales and two dwarf sperm whales. Sites of collection included stomach, nasal/pterygoid, peribullar sinuses, blubber, and abdominal cavity. Parasite species, locations and loads were within normal limits for free-ranging cetaceans and were not considered causative for the stranding event. Gas emboli lesions which were considered consistent with or diagnostic of sonarassociated strandings of beaked whales or small cetaceans were not found in the whales stranded as part of UMESE0501Sp. Twenty-five heads were examined with nine specific anatomic locations of interest: extramandibular fat, intramandibular fat, auditory meatus, peribullar acoustic fat, peribullar soft tissue, peribullar sinus, pterygoid sinus, melon, and brain. The common finding in all examined heads was verminous pterygoid sinusitis. Intramandibular adipose tissue reddening, typically adjacent to the vascular plexus, was observed in some individuals and could represent localized hemorrhage resulting from vascular rete rupture, hypostatic congestion, or erythrocyte rupture during the freeze/thaw cycle. One cetacean had peracute to acute subdural hemorrhage that likely occurred from thrashing on the beach post-stranding, although its occurrence prior to stranding cannot be excluded. Information provided to NMFS by the U.S. Navy indicated routine tactical mid-frequency sonar operations from individual surface vessels over relatively short durations and small spatial scales within the area and time period investigated. No marine mammals were detected by marine mammal observers on operational vessels; standard operating procedure for surface naval vessels operating mid-frequency sonar is the use of trained visual lookouts using high-powered binoculars. Sound propagation modeling using information provided to NMFS indicated that acoustic conditions in the vicinity likely depended heavily on position of the receivers (e.g., range, bearing, depth) relative to that of the sources. Absent explicit information on the location of animals meant that it was not possible to estimate received acoustic exposures from active sonar transmissions. Nonetheless, the event was associated in time and space with naval activity using mid-frequency active sonar. It also had a number of features in common (e.g., the “atypical” distribution of strandings involving multiple offshore species, all stranding alive, and without evidence of common infectious or other disease process) with other sonar-related cetacean mass stranding events. Given that this event was the only stranding of offshore species to occur within a 2-3 day period in the region on record (i.e., a very rare event), and given the occurrence of the event simultaneously in time and space with a naval exercise using active sonar, the association between the naval sonar activity and the location and timing of the event could be a causal rather than a coincidental relationship. However, evidence supporting a definitive association is lacking, and, in particular, there are differences in operational/environmental characteristics between this event and previous events where sonar has apparently played a role in marine mammal strandings. This does not preclude behavorial avoidance of noise exposure. No harmful algal blooms were present along the Atlantic coast south of the Chesapeake Bay during the months prior to the event. Environmental conditions, including strong winds, changes in upwelling- to downwelling-favorable conditions, and gently sloping bathymetry, were consistent with conditions which have been correlated with other mass strandings. In summary, we did not find commonality in gross and histologic lesions that would indicate a single cause for this stranding event. Three pilot whales and one dwarf sperm whale had debilitating conditions identified that could have contributed to stranding, one pilot whale had a debilitating condition (subdural hemorrhage) that could have been present prior to or resulting from stranding. While the pilot and dwarf sperm whale strandings may have had a common cause, the minke whale stranding was probably just coincidental. On the basis of examination of physical evidence in the affected whales, however, we cannot definitively conclude that there was or was not a causal link between anthropogenic sonar activity or environmental conditions (or a combination of these factors) and the strandings. Overall, the cause of UMESE0501Sp in North Carolina is not and likely will not be definitively known. (PDF contains 240 pages)

Reproduction, maturation, and seed production of cultured species: Proceedings of the Twelfth U.S.-Japan Meeting on Aquaculture, Baton Rouge, Louisiana, October 25-29, 1983

The United States and Japanese counterpart panels on aquaculture were formed in 1969 under the United States-Japan Cooperative Program in Natural Resources (UJNR). The panels currently include specialists drawn from the federal departments most concerned with aquaculture. Charged with exploring and developing bilateral cooperation, the panels have focused their efforts on exchanging information related to aquaculture which could be of benefit to both countries. The UJNR was begun during the Third Cabinet-Level Meeting of the Joint United States-Japan Committee on Trade and Economic Affairs in January 1964. In addition to aquaculture, current subjects in the program include desalination of seawater, toxic microorganisms, air pollution, energy, forage crops, national park management, mycoplasmosis, wind and seismic effects, protein resources, forestry, and several joint panels and committees in marine resources research, development, and utilization. Accomplishments include: Increased communication and cooperation among technical specialists; exchanges of information, data, and research findings; annual meetings of the panels, a policy-coordinative body; administrative staff meetings; exchanges of equipment, materials, and samples; several major technical conferences; and beneficial effects on international relations. (PDF file contains 79 pages.)

Preliminary studies of the dual role of cotton-seed cake as fish feed and fish pond fertilizer in aquaculture

Many locally available fish feeds have been tried in fish culture. These include guinea corn, soya bean, groundnut cake and rice bran. Cotton seed cake has been successfully used as a fishpond organic fertilizer at Ado-Ekiti Government fish farm. Three fishponds stocked respectively with common carps, Heterotis, Tilapia spp., Clarias lazera and Heterobranchus were fed with cotton seed cake. Carps, Tilapia and Heterotis increased rapidly in weight and length while the catifishes did not grow

Small scale commercial production of tilapia fingerlings in floating bamboo net-hapas

The inadequate supply of tilapia seed is considered as one of the major present constraints to the development of the culture industry in Nigeria. The floating bamboo net-hapa hatchery/nursery system was observed to be very efficient in the mass production of tilapia (Oreochromis niloticus) fry and fingerlings at Kainji Lake Research Institute. This system was therefore, recommended for small-scale (artisanal) commercial operators consisting of fishermen families in order to increase their productivity and hasten development of rural communities. The economic analysis of this system showed that loan obtained for the recommended scale of operation can be amortized within 2 years of the project. It was emphasized that the operational and managerial skills of the fish farm operators account largely to the production cost and profitability of the enterprise

Investment opportunities in fish seed technology

An examination is made of the requirements for the commercial propagation of carp and tilapia in Nigeria. It is concluded that the operation of a successful Fish Hatchery and fry production system will depend on the following factors: 1) Correct initial planning for intended production (both species and intended numbers); 2) Design of the appropriate facilities to enable required production; 3) Selection of top calibre, dedicated and experienced hatchery staff; and, 4) The ethical responsibility taken by the hatchery management to produce only the highest quality seed under the best possible conditions. Purchasing farmers are dependent on this

Feed conversion, protein efficiency, digestibility and growth performance of Oreochromis niloticus fed Delonix regia seed meal

Oreochromis niloticus fingerlings (mean weight 5.27~c0.29g) were fed raw and boiled Delonix regia seed meals following standard procedures. The weight gain, specific growth rate (SGR), feed conversion ratio (FCR), protein efficiency ratio (PER), net protein utilization (NPU) were determined as growth indices. Diet formulated with seed boiled for 80 minutes showed significantly (P<0.05) high values for the growth indices. Carcass nutrients composition were significantly (P<0.05) higher than in the control (raw) diet. Delonix regia seed meal when boiled has high potential of being utilized efficiently by O.niloticus. The implications of the respective index in fish metabolism are discussed

Potentials of castor seed meal (Ricinus communis L.) as feed ingredient for Oreochromis niloticus

The potentials of castor seed (Ricinus communis) meal as feed ingredient for Oreochromis niloticus was determined by using boiled seeds to prepare five diets which were fed to the fish species. The effects of the experimental diets on the weight gain, specific growth rate, feed conversion ratio, protein efficiency ratio, apparent net protein utilization, digestibility and carcass composition were investigated. The best of these growth factors were obtained with feed formulated from R. communis seeds boiled for 50 and 65 minutes. The highest carcass protein content was observed with fish fed with 65 minute-boiled seeds

Mechanism of mass transfer in the formation of hydrothermal deposits of sulphides [Translation from: Trudy Inst.Geol.rudn.Mesterozh. 16, 80 [pages 44-46 ”Solubility of sulphides of iron” only], 1958]

This partial translation of the original paper provides the summary of this study of the mechanism of mass transfer in the formation of hydrothermal deposits of sulphides. For determining the solubility of sulphides of iron, the radioactive isotope Fe59 was used. The solubility of two sulphides was determined.