Liver genomic responses to ciguatoxin: evidence for activation of Phase I and Phase II detoxification pathways following an acute hypothermic response in mice

Ciguatoxins (CTX) are polyether neurotoxins that target voltage-gated sodium channels and are responsible for ciguatera, the most common fish-borne food poisoning in humans. This study characterizes the global transcriptional response of mouse liver to a symptomatic dose (0.26 ng/g) of the highly potent Pacific ciguatoxin-1 (P-CTX-1). At 1 h post-exposure 2.4% of features on a 44K whole genome array were differentially expressed (p ≤ 0.0001), increasing to 5.2% at 4 h and decreasing to 1.4% by 24 h post-CTX exposure. Data were filtered (|fold change| ≥ 1.5 and p ≤ 0.0001 in at least one time point) and a trend set of 1550 genes were used for further analysis. Early gene expression was likely influenced prominently by an acute 4°C decline in core body temperature by 1 h, which resolved by 8 h following exposure. An initial downregulation of 32 different solute carriers, many involved in sodium transport, was observed. Differential gene expression in pathways involving eicosanoid biosynthesis and cholesterol homeostasis was also noted. Cytochrome P450s (Cyps) were of particular interest due to their role in xenobiotic metabolism. Twenty-seven genes, mostly members of Cyp2 and Cyp4 families, showed significant changes in expression. Many Cyps underwent an initial downregulation at 1 h but were quickly and strongly upregulated at 4 and 24 h post-exposure. In addition to Cyps, increases in several glutathione S-transferases were observed, an indication that both phase I and phase II metabolic reactions are involved in the hepatic response to CTX in mice.

1987 ecosystem view of management research in the Myakka River: review of Phase I and Phase II reports

This memorandum has four parts. The first is a review and partial synthesis of Phase 1 and Phase 2 Reports by Dr. Ernest Estevez of the Mote Marine Laboratory to the Board of County Commissioners of Sarasota County, Florida. The review and synthesis emphasizes identification of the most important aspects of the structure of the Myakka system in terms of forcing functions, biological components, and major energy flows. In this context, the dominant primary producers, dominant fish species and food habits, and major environmental variables were of articular interest. A major focus of the review and synthesis was on the river zonations provided in the report and based on salinity and various biological indicators. The second part of this memorandum is a review of a draft report by Mote Marine Laboratory on evaluation of potential water quality impacts on the Myakka River from proposed activities in the watershed. This Memorandum's third part is a review of resource-management related ecosystem models in the context of possible future models of the Myakka River Ecosystem. The final part of this memorandum is proposed future work as an extension of the initial reports.

Reproduction, movements, and population dynamics of the southern kingfish, Menticirrhus americanus, in the northwestern Gulf of Mexico

Menlicirrhus americanus in the northwestern Gulf of Mexico mature at 150-220 mm TL and 12-14 months of age, with males maturing when 10-40 mm smaller than females. Spawning occurs within a broad period from February through November with two discrete peaks which coincide with the periodicity of downcoast alongshore currents (towards Mexico) in spring and fall. This species occurs at depths of less than 5 to 27 m, being most abundant at 5 m or shallower. Young-of-the-year recruit primarily at 5-9 m or shallower and gradually expand their bathymetric range. Age determination by length frequency is feasible in M. americanus but not as simple as in species that spawn in one major period of the year. Only one or two spawned groups normally predominated at anyone time and no more than three co-occurred with few possible exceptions. Observed mean sizes were 138 mm TL at 6 months, and 192 and 272 mm at ages I and II, respectively. Typical maximum size was 296-308 mm and typical maximum age is probably 2-3 years. The largest fISh captured were 392 and 455 mm. Observed sex ratio was 1.2 females to 1 male. Weight, girth, and length-length regressions are presented.(PDF file contains 27 pages.)

Growth and mortality of dagaa (Rastrineobola argentea, Fam. Cyprinidae) in Lake Victoria

Growth and mortality parameters of the small Lake Victoria cyprinid Rastrineobola argentea were determined from length-frequency analysis, using the ELEFAN I and II programs. The results of two sampling programs, both performed during 1988, one in Uganda (mosquito seine) and the other in Tanzania (pelagic trawl), were highly corresponding, In comparison with previously published data on the growth of dagaa and some similar species, low values for L sub( infinity ) (65 mm standard length) and K (1 year super(-1)) were found. Total mortality (Z) amounted to 3.9-4.4 year super(-1). A single annual breeding peak was observed both in Uganda (October/November) and in Tanzania (February/March).

Distributional patterns of brachyuran larvae in Manora Channel (Karachi, Pakistan) collected during 1995

This study gives an account of distributional patterns of Brachyuran larvae in the Manora Channel from January to November 1995. The planktonic sampling was carried out during day time from surface and sub-surface waters of station I and II (certain sites) at shallow depths (15'-20') using Bongo net of 300 micron mesh size. In all 19527 larvae were obtained through fourteen sampling. These brachyuran larvae belonged to nine families and twenty four species: Ebalia sagittifera, Philyra sp., Philyra scabriuscula (Leucosiidae), Schizophyris aspera (Majidae), Charybdis annulata, Charybdis sp. (Portunidae), Xanthid sp A., B. and C. (Xanthidae), Pilumnus karachiensis, Pilumnus sp. (Pilumnidae), Menippe rumphii (Oziidae), Pinnotheres sp. A, and B. (Pinnotheridae), Nasima dotilliforme, Serenella indica, Macrophthalmus (Mareotis) depressus, Macrophthalmus sp., Dotilla blanfordi, Ocypodid sp. A., B. and C. (Ocypodidae), Metopograpsus thukuhar and Clistocoeloma lanatum (Grapsidae). This study is based on identification, occurrence, distributional patterns along Manora Channel and percentage composition of brachyuran larvae in the area, collected during 1995.

A report of the bottom trawl monitoring survey of Lake Victoria, Uganda, September-October 2007

Under the implementation of the fisheries management plan (IFMP) for Lake Victoria result area 4, bottom trawl monitoring surveys are undertaken to monitor changes in the status of the fish stocks and environment of Lake Victoria. Trawl together with the lake-wide Hydroacoustic surveys give a reflection on the status of fish stocks in the lake to guide management decisions The National working group for bottom trawl surveys in Uganda undertakes these surveys within the Uganda sector of Lake Victoria. For the purpose of this survey, the Uganda sector of Lake Victoria is divided into three zones. A cruise lasting twenty days is conducted in two phases (two legs) 13 days covering Zones I and II and 7 days in Zone III.