Effect of substituting animal protein sources with soybean meal in diets of Oreochromis karongae (Trewavas 1941)

Three diets were formulated using locally available feed ingredients in Malawi to test the effect of replacing animal protein (fish meal, meat and bone meal) with soybean meal (10:0, 5:5, 0:10% of diet) as the protein source on growth and feed conversion of Oreochromis karongae. There were no significant differences in growth rate (GR), specific growth rate (SGR) and feed conversion ratios (FCR) among the three diets. It can be concluded that more expensive and limited animal protein sources can totally be replaced by cheaper soybean in order to get similar growth rates in O. karongae.

Effects of animal and plant protein diets on growth of Indian major carp (Labeo rohita Ham.) fingerlings

An experiment was conducted with Labeo rohita fingerlings in an indoor static fish rearing water system of glass made aquaria. Five experimental diets A, B, C, D and E were formulated containing 33% dietary protein level in five treatments each having two replicates containing 12 fingerlings of mean total initial weight of 13.00±0.2g. Sixty days of feeding trial in this experiment showed that fish fed on diet 'A' containing fish meal and diet 'E' containing mixed plant sources protein had significantly highest and lowest growth respectively. However, no significant difference of growth was found in fish fed on diets C and D containing meat and bone meal, and mix of animal protein source diets respectively. The result showed that the apparent protein digestibility (APD) of diets 'A' and 'E' had significantly best and least values respectively. Food conversion ratio (FCR) and protein efficiency ratio (PER) ranged between 1.37 to 2.17 and 1.38 to 2.18 respectively. On the basis of observed FCR and PER diets 'A' and 'E' produced significantly highest and lowest growth respectively.

Development of low cost feed using local ingredients for culture of freshwater prawn, Macrobrachium rosenbergii in ponds

Two experiments were conducted to formulate and evaluate test diets using locally available ingredients to find out suitable diets for mono and polyculture of Macrobrachium rosenbergii in ponds. The first experiment was conducted from 1 July 2003 to 29 September 2003 in 12 experimental ponds each measuring 30 square meters behind the Fisheries Faculty Building, BAU campus, Mymensingh. Three experimental diets containing 30% protein were formulated using fish meal, meat and bone meal, mustard oilcake, sesame meal and rice bran and assigned to treatments 1, 2 and 3, respectively. A special shrimp feed (Starter-II) from Saudi-Bangla Fish Feed Ltd. was assigned to treatment 4 (Control). Each treatment had three replications. Juveniles of M. rosenbergii (2.90±0.81g) were stocked at the rate of 4/square meter. Prawns were fed three times daily at the rate of 15% of their body weight at the beginning, which was gradually reduced to 10% and 5% for the last two months. There was no significant (P>0.05) difference between the weight gains of prawns fed diets 1 and 4 (control), but they were significantly (P<0.05) higher than those of diets 2 and 3. The FCR values of diets ranged between 2.61 to 3.36 with diets 1 and 4 showing significantly (P<0.05) lower FCR values. The survival rate of prawns ranged between 68 to 78% with prawns fed diets 1 and 4 showing significantly higher survival rate. The production of prawn ranged from 921 to 1,428 kg/ha/90 days and diet 1 gave the highest production. Treatment 1 gave the highest net profit ofTk. 161,980/ha/90 days. The second experiment was conducted from December, 2003 to April, 2004 to see the growth of over wintered M. rosenbergii juveniles in polyculture with indigenous major carps (catla and rohu) in ponds using formulated diets. Three isoenergetic experimental diets formulated using fish meal, meat and bone meal, mustard oil cake, rice bran, wheat bran and molasses, and a shrimp feed 'Golda special feed' from Saudi-Bangla Fish Feed Ltd. were assigned to treatments 1, 2, 3 and 4 (control), respectively, each treatment had two replications. Eight experimental ponds each measuring 80 square meters in the Field Laboratory Complex of the Faculty of Fisheries, BAU campus, Mymensingh were used. The mean initial weights of M. rosenbergii, catla and rohu were 1.60±0.01, 30.0±0.09 and 25.0±0.08 g, respectively. A total of 160 fish and prawn (20,000/ha) were stocked in each pond at the ratio of 2:1:1 (prawn: catla: rohu). Fish were fed at the rate of 3-5% of their body weight. Prawns fed diet 1, 2 and 4 showed higher weight gains compared to diet 3. The weight gain of catla was significantly higher in T1 while in case of rohu was higher in T1 and T4, respectively. There were no significant differences (P>0.05) in the survival rate of fish as obtained from different treatments. The overall total fish production ranged from 2196 to 2679 kg/ha/5 months. The highest production and the highest profit (Tk. 56,531/ha/5 months) was obtained from T1 and the lowest (Tk. 24,932/ha/5 months) from T4.

An investigation of the effect of replacing fish meal with soyabean meal, groundnut cake and blood meal at varied proportion on growth and food utilization of the Clarias anguillaris fingerlings fed in outdoor hapas

Fingerlings of Clarias anguillaris obtained from a homogenous source through induce breeding and each with a mean weight of 2.8g were stocked in ten hapas each measuring 1.0x1.0m in outdoor concrete tank and were fed for eight (8) weeks. Results shows that the best growth rate was recorded among fingerlings fed fish meal as the only protein source (TD5) while DT2 containing soya bean, groundnut cake (40%), blood meal as the protein sources came next. The growth rate of fingerlings fed DT2 (40 % groundnut cake, 10% soyabean meal and 10% blood meal) was higher than those fed DT4 containing 10% fish meal but lower than those fed DT5 which has fish meal as its sole source of protein (53.0%). Analysis of various growth parameters like SGR, FCR and PER. shows that DT5 was the overall best diet but there was no significant statistical difference in weight gained by fish fed the five diets (P <0.05)

Estimation of growth and financial analysis through the application of Ipil ipil (Leucaena leucocephala) leaf meal as supplements to soybean and fish meal in the diet of juvenile monosex tilapia (Oreochromis niloticus)

Among plant protein ingredients,ipil ipil (Leucaena leucocephala) leafmeal (ILLM) is considered the most nutritive plant protein source after soybean meal in aquatic feeds. That was proven in a 21-day experiment conducted to assess the response of juvenile Monosex Nile tilapia Oreochromis niloticus with four iso-nitrogenous formulated diets: One control diet was formulated based on fishmeal, one on soybean meal and one on rice bran, ipil ipil leafmeal was also included in experimental diets.

Growth and survival of Penaeus monodon postlarvae fed shrimp head meal and fish meal as primary animal source of protein

Although shrimp head meal alone does not provide for good growth and survival, fish meal can provide high survival rate. The addition of shrimp head improves this diet. It is suggested that cholesterol present in shrimp could have caused this difference. Composition of the test diets is tabulated, as are proximate chemical analysis of the diets, and the mean initial weights, final weights, weight gains, survival rate, feed consumed, protein consumed, of Penaeus monodon postlarvae, feed conversion and protein efficiency ratio.

Studies on shell and meat composition of molluscs from Saurashtra coast

Shell composition of 35 species of molluscs was studied. The shell, calcium and calcium carbonate contents were lowest in the cuttle bone of cuttlefish, while they were highest in shell of Cypraea ocellata. Solen sp. had higher meat and protein content than the pelecypods and lower meat, water and protein content than the cephalopods. Inverse relationships between water and fat, and protein and ash were established.

A preliminary study on the evaluation of casein, shrimp meal, squid meal and spirulina as protein sources of Penaeus monodon (Fabricius) postlarvae

P. monodon postlarvae were fed with fresh brown mussel (Modiolus metcalfei ) meat, and artificial diets containing casein, shrimp (Metapenaeus ensis ) meal, squid (Loligo ) meal, and Spirulina as protein sources at a rate of 20% of their biomass per day for a period of 10 days. No statistical difference was found among the weight gains of shrimp fed on various experimental diets. However, shrimp fed squid meal diet had highest weight gain followed by shrimp meal, fresh brown mussel meat, casein and Spirulina . Shrimp fed squid meal diet had a significantly better feed conversion and those receiving other experimental diets. Poorest fed conversion was observed in shrimp fed Spirulina . The protein efficiency ratio was statistically highest for the squid meal diet, with the lowest value again for Spirulina diets. Survival rate was significantly highest for shrimp fed the shrimp meal diet. No statistical differences were found among shrimp fed other experimental diets. Appropriate data are tabulated.

Application of ipil- ipil leaf meal (IILM) as feed Ingredient for Monosex tilapia fry (oreochronis niioticus) : In terms of growth and economics

The Ipil-ipil (Leucaena leucocephal) leaf analyzed for crude protein (CP), fat, crude fibre (CF), ash content, moisture content and nitrogen free extract (NFE). The CP 23± 0.12 % , fat 8 ± 0.11 %, CF 18 ± 0.15 % , ash 10 ± 0.13 %, moisture 14. ± .16% and NFE 29.± 1.10 % were recorded. A twenty one days experiment was conduced to assess the response of juvenile monosex tilapia with different iso-nitrogenous formulated diets for find out the feasibility study of using ipil-ipil leaf meals as feed ingredient for juvenile tilapia. Three experimental diets were formulated by using fish meal, soybean meal, rice bran and ipil ipil leaf meal. One control diet was formulated by using fish meal, soybean meal and rice bran. Considering the high demand, limited availability of fish meal and soybean meal, ipil ipil leaf meal was incorporated in juvenile tilapia feed. Among plant protein ingredients ipil ipil leaf meal was considered as the most nutritive plant protein source after soybean meal. However, high concentration of toxic element limited the incorporation level of ipil ipil leaf meal in fish feed. Use of 15 % ipil ipil leaf meal in fish feed was more significant from the view of growth performance and economics. The higher Absolute Growth was 1119.26 gm, higher Specific Growth Rate was 6.52% /day higher Feed Conversion Efficiency was 41.23% , higher Protein Efficiency Ratio was 1.178 and higher Average Daily Growth rate was 14.00% recorded in diet-4 ( which contained 15% IILM). The lower Feed Conversion Ratio 2.42 and lower cost for per unit production 34.65 taka/kg were recorded in diet-4. The higher cost for per unit fish production 45.6 tk./kg was recorded for diet-1 where no ipil ipil leaf meal.. The results suggest that tree legumes Ipil-ipil (Leucaena leucocephal) leaf has potential and excellent source of feed ingredients as protein supplements for juvenile monosex

Digestible protein and energy value of fish meal, dextrin, fish oil and soybean oil for Thai sharpunti (Puntius gonionotus Bleeker)

A laboratory trial was conducted to determine the digestible protein and energy value of fish meal, dextrin, fish oil and soybean oil for Thai sharpunti (Puntius gonionotus Bleeker). A reference diet containing 35% protein was formulated in which fish meal was the sole source of protein. Five test diets were formulated using reference diet and individual test ingredients (fish meal, dextrin, fish oil and soybean oil). Each treatment had three replicates with 15 fish per replicate. Fish were fed twice daily at the rate of 5% of their body weight. The result of the study indicated that the dietary protein in both reference and test diets were well digested and the apparent protein digestibility (APD) values of test diets ranged between 82.81 and 85.99%. The APD value of fish meal protein was 88.05%. The apparent digestible energy (ADE) value for the test ingredients ranged between 70.79 and 85.80% with soybean oil having the highest and fish meal the lowest value. The ADE values calculated in terms of Kcal/g of ingredients were 3.68, 3.22, 4.38 and 4.44 Kcal/g for fish meal, dextrin, fish oil and soybean oil respectively.

Effects of dietary phosphorus and zinc levels on growth artd bone mineralization in fingerlings of rainbow trout, Oncorhynchus mykiss

A laboratory based 2 x 2 factorial experiment was conducted to investigate the influences of dietary phosphorus and zinc levels on growth and bone mineralization in fingerlings of rainbow trout for 21 weeks. Two levels of phosphorus (19 and 30 mg/g) and two levels of zinc (55 and 103 Ag/g) in the dry diets were tested. Duplicate tanks of 30 rainbow trout (average weight 1.56 ± 0.24 g) per 60L glass tank were fed experimental diets three times a day to apparent satiation level at 15 to 24°C water temperature. The results of the present study demonstrated that dietary phosphorus supplementation influenced the growth and bone mineralization whereas zinc levels significantly (p<0.05) influenced bone mineralization in rainbow trout. Further investigations in this area with different size and age groups of this fish are broadly needed.

Input-output relationship and economics of pangas monoculture and carp-pangas polyculture in two districts of Bangladesh

An attempt was made to study the input-output relationships and economics of pangas monoculture and carp-pangas polyculture in Bangladesh. By analyzing the data collected from 50 pangas farms and 55 carp-pangas farms, the study has investigated the production systems of two technologies and the effects of fingerling stocking and applications of feed and fertilizer on fisheries income. The data were collected from the fishermen of Trishal and Bhaluka of Mymensingh district, and Kahaloo and Adamdighee of Bogra district during 2001-02. For pangas monoculture, the stocking density was 31,561 per ha while it was 55,017 per ha in carp-pangas polyculture. Most of the farmers used urea, TSP and lime before stocking. Rice and wheat bran happened to be the most common feed ingredients for both types of culture in general. Other important ingredients used were mustard oil-cakes, rice polish, wheat flour, fish meal, bone meal, soybean meal and poultry litter. In terms of quantities, rice bran and wheat bran dominated the farmers list. Rice and wheat bran together constituted about 60% of all studied feeds. Feed cost constituted 59.13% of total costs for pangas monoculture and 67.44% for carp-pangas polyculture. Per ha productions of pangas and carp-pangas in a single culture cycle were 15,508 kg and 19,745 kg, respectively. Per ha gross profits were estimated to be Tk 310,311 and Tk 464,418 for pangas monoculture and carp-pangas polyculture, respectively. Net profit appeared to be Tk 264,216 per ha for pangas monoculture and Tk 416,509 per ha for carp-pangas polyculture. The BCRs calculated were 1.46 and 1.68 for monoculture and polyculture, respectively. The break-even costs per kg of fish were estimated at Tk 36.93 for pangas and Tk 30.93 for mixed species which was much lower than the prices the producers received. Break-even productions were estimated at 10,702 kg per ha for pangas monoculture and 11,784 kg per ha for carp-pangas polyculture. Fingerling and feed cost, and pond size significantly explained the variation of income from pangas monoculture. These factors have significantly influenced the income from the crop. Functional analysis shows that 1% increase in the feed cost might increase 0.51% of pangas income and 0.41% in carp-pangas income. No other inputs had shown this much of responses to increasing income from a fish.

Effect of fish meal in formulated diet on the growth of Heterotis niloticus in semi-intensive pond culture system

The aim of the investigation is to know the percentage of fish meal required to support the best growth of Heterotis niloticus in a semi intensive pond culture system. To achieve this, feed was formulated with equal percentages of blood meal, and corn meal and varying levels of fish meal. The experiment was in four treatments. Results showed that the mean weight gained was directly proportional to the quantity of fish meal made available to the fish fence 31.58g, 33.79g, 45.15g and 51.24g were recorded for treatments I, II, III and IV respectively. Result from this study when compared with previous works, shows that size of the water body to a greater extent affects the growth. The availability of fish meal in the feed made it more acceptable to the fish and hence a commensurate conversion in to flesh. The analysis of variance showed that there is significant difference in the growth performance in the treatments

The effect of replacement of soyabean meal with blood meal on the growth of mudfish Clarias anguillaris (L) fingerlings

Blood meal and full fat soyabean meal were mixed in different proportions to give 0%, 10%, 25%, 50%, 75%, 90% and 100% meal in the protein fraction of the diet and fed to Claria anguillaris fingerlings in floating hapas. The growth performance of the fingerlings were monitored for 84 days. At the end of the experiment the mean weight of the fingerlings increased in the level of blood meal up to 50% blood meal in the diet after which there was a decline in the mean weight of the fish. This same level of blood meal gave the best specific growth rate, feed conversion efficiency and protein efficiency ratio. Thus the nutritive value of blood meal was enhanced by the addition of an equal level of full fat soyabean meal in the diet

Reconstruction of original body size and estimation of allometric relationships for the longfin inshore squid (Loligo pealeii) and northern shortfin squid (Illex illecebrosus)

Quantification of predator-prey body size relationships is essential to understanding trophic dynamics in marine ecosystems. Prey lengths recovered from predator stomachs help determine the sizes of prey most influential in supporting predator growth and to ascertain size-specific effects of natural mortality on prey populations (Bax, 1998; Claessen et al., 2002). Estimating prey size from stomach content analyses is often hindered because of the degradation of tissue and bone by digestion. Furthermore, reconstruction of original prey size from digested remains requires species-specific reference materials and techniques. A number of diagnostic guides for freshwater (Hansel et al., 1988) and marine (Watt et al., 1997; Granadeiro and Silva, 2000) prey species exist; however they are limited to specific geographic regions (Smale et al., 1995; Gosztonyi et al., 2007). Predictive equations for reconstructing original prey size from diagnostic bones in marine fishes have been developed in several studies of piscivorous fishes of the Northwest Atlantic Ocean (Scharf et al., 1998; Wood, 2005). Conversely, morphometric relationships for cephalopods in this region are scarce despite their importance to a wide range of predators, such as finfish (Bowman et al., 2000 ; Staudinger, 2006), elasmobranchs (Kohler, 1987), and marine mammals (Gannon et al., 1997; Williams, 1999).