Artificial fertilization of the eggs, and rearing and identification of the larvae of the anchoveta, Cetengraulis mysticetus

ENGLISH: The anchoveta, Cetengraulis mysticetus (Günther), is an important bait fish used to capture tunas in the Eastern Tropical Pacific Ocean. Contributions to the early life history of this species in the Gulf of Panama were made by Simpson (1959), who was able to identify deductively the planktonic egg of the anchoveta from 10 other anchovy eggs concurrently present. He also reared these planktonic eggs in the laboratory and described the resultant larvae to the age of 48 hours after hatching. Because of the lack of differences among the anchovy larvae, this description does not permit the identification of anchoveta larvae from those of other engraulid species. Furthermore, while adult specimens are easily recognized, up to the present it has not been possible to extend the identification of the juvenile anchoveta to specimens smaller than about 25 mm. The purpose of this study, therefore, was to identify anchoveta from the time of hatching to about 25 mm. SPANISH: La anchoveta, Cetengraulis mysticetus (Günther), es un importante pez de carnada que se emplea en la captura de los atunes en el Océano Pacífico Oriental Tropical. Simpson (1959) logró identificar deductivamente el huevo planctónico de la anchoveta al separarlo de otros diez huevos de anchoas que se encuentran al mismo tiempo, contribuyendo de esta manera a conocer los primeros estados de la historia natural de esta especie en el Golfo de Panamá. El también estableció un criadero en el laboratorio con estos huevos planctónicos y describió las larvas resultantes hasta la edad de 48 horas después de la eclosión. Debido a que no hay diferencias entre las larvas de las anchoas, esta descripción no permite identificar las larvas de la anchoveta de las otras especies de engráulidos. Más aun, a pesar de que los especímenes adultos son fácilmente reconocibles, hasta ahora no ha sido posible identificar la anchoveta juvenil de menos de unos 25 mm. Consecuentemente, el propósito del presente estudio ha sido el de identificar al anchoveta desde el momento de la eclosión hasta que tiene unos 25 mm.

Description of Early Larvae of Four Northern California Species of Rockfishes (Scorpaenidae: Sebastes) from Rearing Studies

About 72 species of Sebastes (Family Scorpaenidae) are found along the eastern Pacific coast of North America, some of which are heavily exploited by both commercial and sport fisheries. Because of the large number of species, the identification of early life stages has progressed slowly. The objectives of this study were 1) to rear the larvae of four species of rockfish (Sebastes mystinus, S. carnatus, S. atrovirens, and S. rastrelliger); and 2) to describe the larvae using morphometric measurements, pigmentation patterns, and head spination. Pigmentation was the most useful feature for identification purposes. Two general patterns were found: 1) a short row of ventral midline melanophores on the tail, and none or very little postero-dorsal pigmentation (S. mystinus); and 2) complete ventral midline pigmentation on the tail, and anterior and postero-dorsal melanophores (S. carnatus, S. atrovirens, and S. rastrelliger). With the exception of very early stages of S. carnatus and S. atrovirens, these species can be readily identified. Morphometric proportions and head spination did not show major differences among species. Because of the great similarities found among species in this genus, descriptions from field studies are uncertain to some extent. Laboratory rearings, although difficult, can at least provide early larvae from known species which allow precise identification as well as an estimation ofvariability of characters (e.g., pigmentation) within and between broods.(PDF file contains 22 pages.)

Design, operation and economics of a small-scale hatchery for the larval rearing of sugpo, Penaeus monodon Fab.

One of the major problems in the mass production of sugpo is how to obtain a constant supply of fry. Since ultimately it is the private sector which should produce the sugpo fry to fill the needs of the industry, the Barangay Hatchery Project under the Prawn Program of the Aquaculture Department of SEAFDEC has scaled down the hatchery technology from large tanks to a level which can be adopted by the private sector, especially in the villages, with a minimum of financial and technical inputs. This guide to small-scale hatchery operations is expected to generate more enthusiasm among fish farmers interested in venturing into sugpo culture.

Rearing cyprinid fry in cages [conclusions only] [Translation from: Gospodarka rybna, Warszawa 27(8/9) 3-5, 1975]

This short translation presents the conclusions only of a study of the proposed method of rearing cyprinid fry in cages and how this method can increase the amount of material produced of the stocked fry.

Catching and rearing postlarval cleaner shrimp for the aquarium trade: results from a WorldFish Center project in Solomon Islands

Between 1999 and 2003, the WorldFish Center in Solomon Islands conducted research into the feasibility of a new fishery based on the capture and culture of postlarval coral reef fish for the live fish trade. The work was carried out in two phases: a research phase from late 1999 to the end of 2002; and a “finetuning” phase in 2003. Most of the species were of value to the marine aquarium trade, with very few live reef food fish recorded. The most valuable ornamentals were the banded cleaner shrimp, Stenopus species. Cleaner shrimp were harvested using crest nets, the method being modified with the addition of a solid, water-retaining cod-end designed to increase survival at capture. Grow-out techniques were improved by rearing the shrimp separately in jars to prevent aggression. The jars were painted black to protect the shrimp from sunlight. An economic model using experimental catch data and farm gate prices indicates that the fishery based on shrimp, supplemented with small numbers of lobster and fish is economically viable. The next step will be setting up a demonstration farm in a village in the Western Province of Solomon Islands.

Isolation, purification, and full NMR assignments of cyclopamine from Veratrum californicum

The Hedgehog signaling pathway is essential for embryogenesis and for tissue homeostasis in the adult. However, it may induce malignancies in a number of tissues when constitutively activated, and it may also have a role in other forms of normal and maladaptive growth. Cyclopamine, a naturally occurring steroidal alkaloid, specifically inhibits the Hedgehog pathway by binding directly to Smoothened, an important Hedgehog response element. To use cyclopamine as a tool to explore and/or inhibit the Hedgehog pathway in vivo, a substantial quantity is required, and as a practical matter cyclopamine has been effectively unavailable for usage in animals larger than mice.

Rearing of major carp spawn at high stocking density under air lift water circulation system

The present studies are aimed to achieve a high survival rate of carp spawn to fry stage under Air Lift Water Circulation system at high stocking density. Three experiments using Labeo rohita and Cirrhinus mrigala were conducted in one cement tank of 50 sq.m. area. The results showed a survival rate ranging from 90.5 to 95.2% at stocking density of 20 to 25 million/ha. By this technique it is possible to rear high number of spawn in limited area with high rate of survival up to fry stage.

Larval rearing of Macrobrachium rosenbergii (De Man) in artificial sea water

Larvae of Macrobrachium rosenbergii were successfully reared in artificial sea water prepared in fresh ground water. The water was circulated through a biological filter by means of air-lift pumps for a period of one week to remove the undissolved particles prior to use in the hatchery operation. The experiments were initiated during 1989 and the hatchery has been working on pilot scale since June, 1990. The larvae in all the experiments were fed with egg-custard, Mona and Artemia nauplii. The survival rate varied from 5 to 52% in the 12 experiments. These findings can add to the development of hatcheries in the inland areas which can further boost the popularization of giant freshwater prawn farming.

Field application of anabolic steroids in carp seed production. Pt. 1. Rearing of fry to fingerling stage

Ethylestrenol (17β Hydroxy-17alpha-ethyl-estr-4-en-3-one) and Stanozolal (17β-Hydroxy-17alpha-methyl 1-5 alpha-androstano-(3,2-C)-pyrazole), both synthetic androgenic steroids, were fed via diet at 3ppm to the fry of catla, rohu and silver carp which were reared up to fingerling stage over a period of 167-172 days in earthen ponds. Ethylestrenol enhanced growth in silver carp and rohu but retarded growth in catla. Stanozolal depressed growth in all the 3 species. Length-weight relationship for these fry had been worked out and the relative condition factor in all the cases was very close to or slightly above 1.0.