A new method for venom extraction from venomous fish, green scat

Scatophagus argus argus (Green Scat) is a pretty aquarium fish. Its hard spines are venomous and can cause painful injury. In this study 60 specimens of Green Scat were collected periodically from coastal waters of Boushehr (south of Iran) from May 2011 to April 2012. Anatomical features of venomous spines were investigated. Scat venom was extracted from the spines in a new manner for keeping the specimens alive. The nature of venom was tested by SDS-PAGE. Ethical issues and animal welfare principles such as rapid and instantaneous anesthetizing, post operation disinfection and fast recovery of the specimens was practiced in order to minimize the complications. This method enhanced the purity and quantity of venom as demonstrated by 12 separated proteins in electrophoresis. New ethical issues were developed to surviving the specimens and prolong viability as well.

Extraction, purification and analysis of conotoxin of Conus textile captured from Persian Gulf and the investigation of analgesic effects of conotoxins in an animal model

Identification of venomous species of Persian Gulf cone snails and characterization of venom composition and their features is so important from the point of medical importance. Marine cone snails from the genus Conus are estimated to consist of up to 700 species. The venom of cone snails has yielded a rich source of novel neuroactive peptides or conotoxins. The present study was aimed to study the analgesic effect of Persian Gulf Conus textile and its comparison with morphine in mouse model. The specimens of Conus textile were collected of Larak Island from depth of 7 m. The collected samples were transferred to laboratory alive and were stored at -700 c. he veno s ducts were separated and ho ogenized with deionized water he ixture centrifuged at rp for inutes upernatant was considered as extracted veno and stored at - C after lyophylization. The protein profile of venom determined by using SDS-PAGE and HPLC used to investigate the extracted venom and to evaluate the analgesic activity, formalin test was carried out. SDS-PAGE indicated several bands ranged between 6 and 250 kDa. Chromatogram of the venom demonstrated more than 44 large and small fractions. The amount of 10 ng of Conus crude venom and analgesic peptide showed the best anti-pain activity in formalin test. No death observed up to 100 mg/kg, which is 250,000 times higher than the effective dose.Venom characterization of Persian Gulf Conus textile may be of medical importance and potential for new pharmaceutical drugs as well.

Interim report on the hydrologic features of the Green Swamp area in Central Florida

The Green Swamp area in central Florida is another area where man is developing agricultural land from marginal land. Though the area is by no means as extensive as that of the Everglades, the present efforts for its development are similar to the early efforts for developing the Everglades in that many miles of canals and ditches have been constructed to improve the drainage. Lest the early mistakes of the Everglades be repeated, the Florida Department of Water Resources considered that an appraisal of the physical and hydrologic features of the area was needed to determine the broad effects of draining and developing the swamp. This reconnaissance provides information required by the State of Florida for determining its responsibility and policy in regard to the Green Swamp area and for formulating future plans for water management of the area. Some of the features that have been determined are: the amount of rainfall on the area; the pattern of surfacewater drainage; the amount and direction of surface-water runoff; the direction of ground-water movement; the interrelationship of rainfall, surface water, and ground water; the effects of improved drainage facilities'; and the effects of the hydrologic environment on the chemical quality of water of the area.(PDF contains 106 pages.)

Some features of the autecology and distribution of chaetognatha in the Eastern Tropical Pacific

ENGLISH: The Inter-American Tropical Tuna Commission, in cooperation with the Tuna Oceanography Research program of the Scripps Institution of Oceanography, is studying in the Eastern Tropical Pacific Ocean methods of identifying waters of different characteristics that may influence the distribution and behavior of the tropical tunas. One method of attacking the problem has been to attempt to use zooplankton species as biological indicators of water masses. It has been demonstrated that certain zooplankters have ecological affinities that make them useful for identifying and tracing the movements of water masses. In the Eastern Pacific Ocean, Bieri (1957), Lea (1955), Le Brasseur (1959), Sund (1959), and Sund and Renner (1959) have presented evidence that certain species of Chaetognatha possibly can serve as indicators. The present work reports on a study of the distributions of species of Chaetognatha, obtained from various depths by means of horizontal closing-net hauls, in relation to concurrent measurements of temperature, salinity, and dissolved oxygen. Analyses of these data have provided a basis for determining which species are of potential use as biological indicators within the area of the Eastern Pacific considered in this study. SPANISH: La Comisión Interamericana del Atún Tropical, en cooperación con el programa de la "Tuna Oceanography Research" de la Institución Scripps de Oceanografía, viene estudiando en el Océano Pacífico Oriental Tropical métodos para identificar aguas de características diferentes que podrían influir en la distribución y en el comportamiento de los atunes tropicales. Uno de los métodos para abordar el problema ha sido el de intentar la utilización de especies zooplanctónicas como índices biológicos de masas de agua. Se ha demostrado que ciertos organismos del zooplancton tienen afinidades ecológicas, merced a las cuales son útiles para identificar y trazar los movimientos de las masas de agua. Bieri (1957), Lea (1955), Le Brasseur (1959), Sund (1959) y Sund y Renner (1959) presentaron evidencia de que ciertas especies de quetognatos pueden servir, posiblemente, como tales índices en el Océano Pacífico Oriental. El presente trabajo informa sobre un estudio de la distribución de las especies de quetognatos obtenidos de distintas profundidades por medio de la red de plancton que se cierra en lanzamientos horizontales y en relación con mediciones concomitantes de la temperatura, la salinidad y el oxígeno disuelto. El análisis de estos datos ofreció una base para la determinación de las especies que son potencialmente aptas para ser usadas como índices biológicos dentro del área del Pacífico Oriental a la cual se refiere este estudio.

Entwicklung einer einfachen und schnellen Analysenmethode für Kohlenmonoxid in Fisch – Teil 2: Methodenoptimierung

After having described in our first paper (Haase et al. 2005) the main features of an easy and fast method for determination of carbon monoxide in fish and the equipment and chemicals necessary as well as first results measured on fish samples, this part deals with the influence of sample preparation, variation of the size of samples, type of solvent, duration of extraction and further conditions on the result of analyses. Both variants of the method are evaluated with regard to measuring expected errors. The single components of the equipment, including prises, are listed to allow a reliable assessment of costs. Additional instrumental colour and DSC measurements on both untreated and CO-treated tuna illustrate the effects.

The diagnostic morphological features of British Sigara striata, S. dorsalis and intermediate specimens (Corixidae), with a new key for identification of adult males

There are 34 species of the family Corixidae (Hemiptera Heteroptera) in Britain and Ireland of which Sigara striata and Sigara dorsalis are the only two British representatives. In this article the authors briefly consider a range of diagnostic features that may be used to separate British specimens of striata from dorsalis. Most of these morphological features have been used in keys to the British species of the subgenus Sigara sensu strictu. A scoring system has also been devised to facilitate the identification of individuals from the southeast of England, although it is applicable to the whole of the British Isles, and a new (short) key is presented.

Some biological features of Saprolegnia parasitica Coker - cause of fish dermatomycosis. [Translation from: Pervaya nauchnaya konferentsiya molodykh Uchenykh Biologov (Tezisy Dokladov) pp.84-86. Kiev, A.N.Ukr. SSR., 1964]

Parasitic and infectious diseases of fish, of wide distribution in fish-rearing ponds, retard to a significant extent the development of fish culture in the Ukraine. One of the diseases of fish attracting attention in connection with the general distribution of its causative agent, the fungus Saprolegnia parasitica Coker, in water-bodies of various types, appears to be dermatomycosis. The aim of this investigation is to study the conditions favouring the development of S. parasitica. Among the studied factors were water temperature and oxygen content.

Bassenthwaite Lake: a general assessment of environmental and biological features and their susceptibility to change

Bassenthwaite (Lake) is one of the larger Cumbrian lakes, certainly one of the most distinctive, and of considerable conservation and amenity value. Although its shores lack sizeable settlements, its main inflow receives sewage effluent from a major tourist centre (Keswick) and is subject to episodic floods. These influences, the growing development of leisure activities at the lake (e.g. sailing, time-share units), and recent road-construction, have led to past appraisals of ecological impacts and lake management. The lake has not been the subject of intense and long-term ecological study, but much scattered information exists that is relevant to future management decisions. In the present Report, commissioned by North West Water, such information - published and unpublished - is surveyed. Especial attention is given to evidence bearing on susceptibility to change, affecting the lake environment and its biota or species of conservation interest. Extensive use has been made of the results of a recent (1986-7) seasonal survey by the FBA.

A general assessment of environmental and biological features of Windermere and their susceptibility to change

Bassenthwaite (Lake) is one of the larger Cumbrian lakes, certainly one of the most distinctive, and of considerable conservation and amenity value. Although its shores lack sizeable settlements, its main inflow receives sewage effluent from a major tourist centre (Keswick) and is subject to episodic floods. These influences, the growing development of leisure activities at the lake (e.g. sailing, time-share units), and recent road-construction, have led to past appraisals of ecological impacts and lake management. The lake has not been the subject of intense and long-term ecological study, but much scattered information exists that is relevant to future management decisions. In the present Report, commissioned by North West Water, such information - published and unpublished - is surveyed. Especial attention is given to evidence bearing on susceptibility to change, affecting the lake environment and its biota or species of conservation interest. Extensive use has been made of the results of a recent (1986-7) seasonal survey by the FBA.

Transitions in the morphological features, habitat use, and diet of young-of-the-year goosefish (Lophius americanus)

This study was designed to improve our understanding of transitions in the early life history and the distribution, habitat use, and diets for young-of-the-year (YOY) goosefish (Lophius americanus) and, as a result, their role in northeastern U.S. continental shelf ecosystems. Pelagic juveniles (>12 to ca. 50 mm total length [TL]) were distributed over most portions of the continental shelf in the Middle Atlantic Bight, Georges Bank, and into the Gulf of Maine. Most individuals settled by 50−85 mm TL and reached approximately 60−120 mm TL by one year of age. Pelagic YOY fed on chaetognaths, hyperiid amphipods, calanoid copepods, and ostracods, and benthic YOY had a varied diet of fishes and benthic crustaceans. Goosefish are widely scattered on the continental shelf in the Middle Atlantic Bight during their early life history and once settled, are habitat generalists, and thus play a role in many continental shelf habit

DNA extraction from formalin-fixed Franciscana tissues

The present paper reports the extraction of DNA from formalin-fixed Pontoporia blainvillei tissues. Following the Vachot and Monerot (1996) protocol, fragmented DNA (300-700bp) was extracted from more than 95% of liver and muscle samples. DNA yield in liver samples was significantly higher than in muscle samples (4.574 ± 1.169mg DNA/mg versus 0.808 ± 0.297mg DNA/mg). Similar results were obtained from nine other species of cetaceans and five species of pinnipeds. It is of special interest to have a method that allows the utilisation of museum specimens not originally preserved for genetic studies, which may include rarely available, declining or extinct species. SPANISH: El presente trabajo reporta la extracción de ADN a partir de tejidos formolizados de Pontoporia blainvillei. Siguiendo el protocolo de Vachot y Monerot (1996) se pudo extraer ADN degradado (300-700pb) en más del 95% de las muestras de hígado y músculo analizadas. El rendimiento en ADN fue significativamente mayor en muestras de hígado que en muestras de músculo (4.574 ± 1.169mg DNA/mg tejido húmedo versus 0.808 ± 0.297mg DNA/mg tejido húmedo). Resultados similares se obtuvieron en otras nueve especies de Cetáceos y cinco de Pinnípedos. Resulta de gran interés contar con un método que permita la utilización de especímenes depositados en museos y que no hayan sido originalmente colectados para estudios genéticos, incluyendo especies de difícil obtención, en franca declinación o extintas.

Assessment of the hydrogeological impacts of aggregate extraction in the Delamere area, Cheshire

This is a technical report on the assessment of the hydrogeological impacts of aggregate extraction activities in the Delamere Area, Cheshire. The first aim of the study was to carry out Stage 3-appropriate assessment, under the EU Habitats Directive (92/43/EEC), of the possible hydrogeological impacts of aggregate extraction activities authorised by the Cheshire CC on candidate Special Areas of Conservation (cSAC) on the Delamere sandsheet, Cheshire. Identifying possible impacts if these activities on the hydrogeological environment, construction of a numerical groundwater flow model of the groundwater system to investigate and quantify impacts and to produce a report as required under Stage 3 of the Habitats Regulations. Secondly, to identify the future potential impacts of the continued extraction of sand and gravel reserves from above and below the water tables from within the Delamere sandsheet, thus releasing reserves identified within the Area of Search of the Cheshire Replacement Minerals Local Plan 1999. This aspect of the study should assist in identifying the implications of further working within Delamere for North West sub-regional apportionment.