A comparative study on the embryonic development of gynogen, triploid, haploid and normal diploid embryos of stinging catfish, Heteropneustes fossilis

UV irradiation and cold shock were applied on the eggs of stinging catfish, Heteropneustes fossilis, to produce haploid,. gynogen and triploid embryos. A comparative account of the various features· of embryonic development in chromosomally manipulated groups viz. haploid, gynogen and triploid and non-manipulated normal diploid group of H fossilis has been discussed. A slow development and delayed hatching were observed in gynogen and triploid embryos compared to those in normal diploid (control) groups. Mass mortality was observed in all chromosomally manipulated groups particularly during the gastrulation stage. The hatchlings of the gynogen, triploid and normal diploid were similar in overall appearance.

On the climatology, oceanography and fisheries of the Panama Bight

ENGLISH: Seasonal changes in the climatology, oceanography and fisheries of the Panama Bight are determined mainly by the latitudinal movements of the ITCZ over the region. Evaporation is about 980 mm annually. Rainfall is probably much less than previous estimates because of a discontinuity in the ITCZ. Freshwater runoff from the northern watershed varies from 22 X 109 m3/mo in October-November to 11 X 109 m3/mo in February-March; from the southeastern watershed it varies from 16 X 109 m3/mo in April-June to 9 X 109 m3/mo in October-December. Total annual runoff is about 350 X 109m3. A marked salinity front is found at all seasons off the eastern shore. In the northern part of the Bight temperatures in the upper layers remained fairly constant from May to November; by February the mean temperature had decreased by 4°C and sharp gradients existed in the geographic distributions. Salinities in the upper layers decreased steadily from May to November; by February the mean salinity had increased by 2.5‰. The mean depth of the mixed layer increased from 27 m in May to 40 m in November; by February upwelling decreased it to 18 m. Between November and February upwelling had doubled the amount of P04-P and tripled that of NO3-N in the euphotic zone; surface phytoplankton production and standing crop, and zooplankton concentrations also doubled during this period. Upwelling was about 1.5 m/mo during May-November and about 9.0 m/mo during November-February, the annual total is about 48 m, Mean primary production is about 0.3 gC/m2day during May-December and about 0.6 gC/m2day during January-April; annual production is about 140 gC/m2. A thermal ridge occurred in February running from the northern to the southwestern part of the Bight. Within this ridge was a marked thermal dome coinciding with the center of the cyclonic circulation cell. Upwelling in the dome averaged 16 m/mo in November-February. The fisheries of the Panama Bight annually produce about 30,000 metric tons of food species and about 68,000 m.t. of species used for reduction. Most attempts to further the understanding of tuna ecology were unsuccessful. The apparent abundances of yellowfin and skipjack in the northern part of the Bight appear to be related to the seasonal cycle of upwelling and enrichment, as abundances are greatest in April and May when food appears to be plentiful. The life-cycle of the anchoveta in the Gulf of Panama also appears to be related to upwelling; the species mass varies from about 39,000 m.t. in December to about 169,000 m.t, in April. About 19.1 X 1012 anchoveta eggs are spawned annually. The life-cycles of shrimp in the Panama Bight appear to be related to upwelling as catches are greatest in May-July, about 3-5 months after peak upwelling, and annual catches are inversely correlated with sea level. SPANISH: Los cambios estacionales en la climatología, oceanografía y pesquerías del Panamá Bight están determinados principalmente por el movimiento latitudinal sobre la región de la Zona de Convergencia Intertropical (ZCIT). La evaporación es de unos 980 mm al año. La pluviosidad es probablemente muy inferior a las estimaciones previas a causa de la descontinuidad en la ZCIT. El drenaje de agua dulce, de la vertiente septentrional, varía de 22 x 109m3/mes en octubre-noviembre hasta 11 x 109m3/mes en febreromarzo; el de la vertiente sudeste varía de 16 x 109m3/mes en abril-junio a 9 x 109m3/mes en octubre-diciembre. El drenaje total, anual, es alrededor de 350 x 109m3. En todas las estaciones frente al litoral oriental se encuentra un frente de salinidad marcada. En la parte septentrional del Bight las temperaturas en las capas superiores permanecieron más bien constantes de mayo a noviembre; en febrero la temperatura media había disminuido en unos 4°C y existieron gradientes agudos en las distribuciones geográficas. Las salinidades en las capas superiores disminuyeron constantemente de mayo a noviembre; en febrero la salinidad media había aumentado en 2.5‰. La profundidad media de la capa mixta aumentó de 27 m en mayo a 40 m en noviembre; en febrero el afloramiento disminuyó el espesor de la capa mixta hasta 18 m. Entre noviembre y febrero el afloramiento había duplicado la cantidad de PO4-P y triplicado la de NO3-N en la zona eufótica; la producción superficial de fitoplancton y la biomasa primaria y las concentraciones de zooplancton también se duplicaron durante este período. El afloramiento era cerca de 1.5 mimes durante mayo-noviembre y de unos 9.0 mimes durante noviembre-febrero, el total anual es de unos 48 m. La producción media primaria es aproximadamente de 0.3 gC/m2 al día durante mayo-diciembre y cerca de 0.6 gC/m2 al día durante enero-abril; la producción anual es de unos 140 gC/m2. En febrero apareció una convexidad termal que se extendió de la parte norte a la parte sudoeste del Bight. Dentro de esta convexidad se encontró un domo termal marcado el cual coincidió con el centro de la circulación ciclonal de la célula. El afloramiento en el domo tuvo un promedio de 16 mimes en noviembre-febrero. Las pesquerías del Panamá Bight producen anualmente de cerca 30,000 toneladas métricas de especies alimenticias y unas 68,000 t.m. de especies usadas para la reducción. La mayoría de los esfuerzos realizados con el fin de adquirir más conocimiento sobre la ecología del atún no tuvo éxito. La abundancia aparente del atún aleta amarilla y del barrilete en la parte septentrional del Bight parece estar relacionada con el ciclo estacional del afloramiento y del enriquecimiento, ya que la abundancia mayor en abril y mayo cuando parece que hay abundancia es de alimento. El ciclo de vida de la anchoveta en el Golfo de Panamá parece también que está relacionada al afloramiento. La masa de la especie varía de unas 39,000 t.m. en diciembre a cerca de 169,000 t.m. en abril. Aproximadamente 19.1 x 1012 huevos de anchoveta son desovados anualmente. Los ciclos de vida del camarón en el Panamá Bight parecen estar relacionados con el afloramiento ya que las capturas son superiores en mayo-julio, unos 3-5 meses después del ápice del afloramiento, y las capturas anuales se correlacionan inversamente con el nivel del mar. (PDF contains 340 pages.)

A statistical investigation of fish prices in Nigeria

The main objective of this study is to describe and characterize the behaviour of fish prices in Nigeria. Drawing upon aspects of the data from a nationwide fish survey in 1980/81 and on various secondary data, the study analyses the pattern of fish price movement and makes projections of fish prices in Nigeria till 2002 A.D. It is concluded that unless efforts are directed at stemming inflation in fish prices, prices paid by fish consumers in Nigeria will be more than doubled within the next two decades

The influence of some environmental variables on the apparent abundance of skipjack tuna, Katsuwonus pelamis, in the Eastern Pacific Ocean

ENGLISH: The abundance of skipjack larvae in the central and western Pacific approximately doubled for every 1°C increase in sea-surface temperature (SST) from 23°C to a maximum of about 29°C, and then usually decreased with further increases in SST. Skipjack larvae are scarce in the eastern Pacific Ocean (EPO), so most skipjack recruits and adults in this area are believed to have originated in the central and, possibly, the western Pacific. The catch per unit of effort (CPUE), in short tons per day's fishing, and the catch rate, in number of fish per day's fishing, are estimates of apparent abundance in a fishery. The logarithm of the annual CPUE for skipjack for international baitboats in the EPO for the 1934-1960 period was positively correlated with SST in the spawning area in the central Pacific 18 months earlier (r2 0.31), during the July-June period when most of the recruits in each cohort were presumed to have been spawned. Adequate data for other environmental variables were not available for testing with the baitboat data. The other environmental variables available and selected for testing for correlation with estimates of skipjack abundance for purse seiners for the 1961-1984 period and the reasons for their selection are as follows. 1)Wind-mixing index (WMI). The degree of mixing in the upper layers of the ocean is proportional to the cube of the wind speed, called WMI. The degree of mixing in the spawning areas of the central and the western Pacific may affect the concentration of organisms that skipjack larvae feed upon, thereby influencing their survival, and ultimately determining cohort strength and the number of recruits to the eastern Pacific fishery. 2) SST in the fishing areas at the time of fishing (SST). The CPUE for yellowfin tuna has been shown to be inversely related to SST in the fishing areas, and there are indications that skipjack CPUE is lower during EI Nino events when SST is higher than normal. 3) North-south SST gradient across the thermal front off the Gulf of Guayaquil. This is a measure of the degree of upwelling and nutrient enrichment of the upper waters south of the front and ultimately of the production of food for tunas. 4) Speed of the North Equatorial Countercurrent (NECC). Young skipjack may migrate from the central Pacific to the EPO in the eastward flowing NECC; if so, the number of recruits might be affected by variations in the speed of the current. The logarithm of the annual catch rate of skipjack recruits by international purse seiners in the EPO for the 1961-1984 period was positively correlated with SST in the spawning area of the central Pacific 18 months earlier (r2 = 0.21),and inversely correlated with WMI in the spawning area 18 months earlier (r2 0.46). The logarithm of CPUE for purse seiners in the area off the Gulf of Guayaquil was not correlated with SST in the spawning area 18 months earlier, but was inversely correlated with WMI in the spawning area 18 months earlier (r2 = 0.19), and inversely correlated with the north-south SST gradient in the fishing area at the time of fishing (r2 0.32). Neither of these estimates of apparent abundance from purse seiners were correlated with SST in the fishing areas, or with the speed of the NECC at earlier times. SPANISH: La abundancia de larvas de barrilete en el Pacífico central y occidental se multiplicó por dos, aproximadamente, por cada aumento de 1°Cen la temperatura de la superficie del mar (TSM) entre 23°C y un máximo de unos 29°C, y luego generalmente disminuyó con más aumentos en la TSM. Las larvas de barrilete son escasas en el Océano Pacífico oriental (OPO), y por lo tanto se cree que la mayoría de los reclutas y adultos en esta zona surgieron del Pacífico central, y posiblemente también del Pacífico occidental. La captura por unidad de esfuerzo (CPUE), en toneladas cortas por día de pesca, y la tasa de captura, en número de peces por día de pesca, son estimaciones de la abundancia aparente en una pesquería. El logaritmo de la CPUE anual de barrilete lograda por barcos de carnada en el OPO en el período 1934-1960 se correlacionó positivamente con la TSM en la zona de desove en el Pacífico central de 18 meses antes (r2 = 0.31), durante el período de junio-julio en el cual se cree que nació la mayoría de los reclutas en cada cohorte. No se dispuso de datos suficientes sobre otras variables ambientales para comprobarlos con los datos de los barcos de carnada. Las demás variables ambientales disponibles y seleccionadas para someterlas a pruebas de correlación con las estimaciones de la abundancia del barrilete de barcos cerqueros en el período 1961-1984, y las razones por su selección, son las siguientes: 1) Indice de mezcla por el viento (IMV). El grado de mezcla en las capas superiores del océano es proporcional al cubo de la velocidad del viento, llamado IMV. Es posible que el grado de mezcla en las zonas de desove del Pacífico central y occidental afecte la concentración de los organismos que alimentan a las larvas del barrilete, afectando así la supervivencia de éstas, y finalmente determinando el tamaño de las cohortes y el número de reclutas a la pesquería del OPO. 2) TSM en la zona de pesca al realizarse la pesca (TSM). Se ha mostrado que la relación de la CPUE del atún aleta amarilla a la TSM en la zona de pesca es inversa, y existen indicaciones que la CPUE de barrilete es inferior durante eventos del Niño, cuando las TSM son superiores a lo normal. 3) Gradiente norte-sur de las TSM a través del frente térmico frente al Golfo de Guayaquil. Esto es una medida del grado de afloramiento y enriquecimiento nutritivo del nivel superior de las aguas al sur de dicho frente, y finalmente de la producción de alimento para los atunes. 4) La velocidad de la Contracorriente Ecuatorial del Norte (CCEN). Es posible que los bariletes juveniles migren del Pacífico central al Pacífico oriental en la CCEN, que fluye hacia el este; de ser así, es posible que la cantidad de reclutas se vea afectada por variaciones en la velocidad de la corriente. El logaritmo de la tasa anual de captura de reclutas de barrilete por cerqueros de varias banderas en el OPO en el período 1961-1964 estuvo correlacionado de forma positiva con las TSM en la zona de desove del Pacífico central de 18meses antes (r2 0.21),y de forma inversa con el IMV de la zona de desove de 18 meses antes (r2 0.46). El logaritmo de la CPUE de los cerqueros en la zona frente al Golfo de Guayaquil no estuvo correlacionado con las TSM en la zona de desove de 18 meses antes, pero sí estuvo correlacionado de forma inversa con el IMV en la zona de desove de 18 meses antes (r2 0.19),y con el gradiente norte-sur de las TSM en la zona de pesca al realizarse la pesca (r2 0.32). Ninguna de estas estimaciones de abundancia aparente provenientes de barcos cerqueros estuvo correlacionada con las TSM en las zonas de pesca o con la velocidad de la CCEN en épocas anteriores. (PDF contains 140 pages.)

Induced diploid meiogynogenesis in the African clariid catfish Heterobranchus longifilis (Valenciennes, 1840) (Pisces: Clariidae)

Diploid meiotic gynogenesis was induced in African catfish, Heterobranchus longifilis by injection of 0.5ml/kg ovaprim on the breeders, followed by application of UV light irradiation on the spermatozoa and temperature shocking of activated eggs. Diploidy was restored by shocking haploid activated eggs at 5 degree C for 40 minutes. The normal control spermatozoa did not receive any UV irradiation nor temperature shock, while the haploid control spermatozoa were irradiated, but did not receive cold shock. The percentage hatchability in the treated group was 25%, while in the control it was 53%. Less than 15 fingerlings had morphological aberrations. After two weeks of indoor rearing, the survival percentage of the treated group was 45% in the control experiment. Cytogenetic analysis of chromosomes revealed 25 chromosomes in the haploid embryo and 50 chromosomes each in diploid gynogenesis and normal diploid control

Study on the biomass yield of Duckweed (Lemna minor) (L.) in hydroponic cultures containing different concentrations of aquaeous extract of chicken manure

The biomass yields of duck week (Lemna minor(L) was monitored in hydroponic media prepared by variously extracting 0.50, 1.00 and 2.00g of dried chicken manure per liter of city water (tap water) supply. The culture media consisting of aqueous extract of the various manure treatments were made up to 12 liters in all cases with tap water as control. Plastic baths of 25 liters capacity with 0.71 super(m2) surface area were used as culture facility. Each bath was stocked at a density of 30g super(m-2) with fresh weed samples (i.e 21.30g/bath). Maximum yields were obtained at all treatment levels and control on day 3 and based on the highest yield of 0.37gm super(-2)d super(-1) (dry matter) obtained at 1.00gL manure treatment which was however not significantly higher (P>0.05) than the 0.36gm super(-2)d super(-1) (dry matter) at 0.05gl super(-1) media manure content, an average manure level of 0.75l super(-1) was selected and used to determine the operational plant density. Thus fresh weights of 30 to 300gm super(-2) was grown in triplicate at 30g intervals for a period of 3 days. A regression equation of Y=2.6720+0.0021x with a corresponding maximum density or operational plant density of 266gm super(-2) and yield of 0.98gm super(-2), d super(-1) (dry matter) were obtained. Further growth trials were carried out at the operational density and manure levels of 0.50, 0.75, 1.00, 1.25, 1.50, 1.75 and 2.00gl super(-1) media manure concentration giving a significantly higher yield (P<0.05) of 17gm super(-2), d super(-1) (dry matter). This yield was however doubled to between 2.21 and 2.24gm super(-2) d super(-1) (equivalent to 7.96 to 8.06mt.ha-1, Yr-1 dry matter on extrapolation) if 25% and 75% respectively of the total weed cover were harvested daily within the experimental period. The role of some dissolved plant nutrients (DPN) were also discussed

Estimating the encounter rate of Atlantic capelin (Mallotus villosus) with fish eggs, based on stomach content analysis

The number of pelagic fish eggs (cod and cunner) found in stomachs of capelin (Mallotus villosus) sampled in coastal Newfoundland was used to estimate the encounter rates between capelin and prey, and thus the effective volume swept by capelin. Fish eggs were found in 4−8% of capelin stomachs, represented an average of 1% of prey by numbers, and their abundance increased as relative stomach fullness decreased. The average number of eggs per stomach doubled for each 5-cm increase in length of capelin. The effective volume swept for eggs by capelin ranged from 0.04 to 0.84 m3/h—a rate that implies either very slow capelin swimming speeds (<1 cm/s) or that fish eggs are not strongly selected as prey. The predation rate estimated from stomach contents was higher than that predicted from laboratory studies of feeding pelagic fish and lower than that predicted by a simple foraging model. It remains uncertain whether capelin play an important regulatory role in the dynamics of early life stages of other fish.

Ecology and growth of juvenile California spiny lobster, Panulirus interruptus (Randall)

ABSTRACT TRANSCRIBED FROM ENGLE'S PH.D. ORAL DEFENSE PAMPHLET: The natural history of juvenile California spiny lobster, Panulirus interruptus (Randall), was investigated, with primary emphasis placed on ascertaining juvenile habitats, determining juvenile growth rates and component growth processes, and evaluating ecological and behavioral phenomena associated with juvenile survival and growth. Habitat surveys of island and mainland localities throughout southern and lower California revealed that small, greenish juveniles typically inhabit crevices or temporary burrows in 0-4m deep, wave-swept rocky habitats covered by dense beds of surf grass, Phyllospadix torreyi S. Watson. Phyllospadix beds were more abundant on gradually sloping rocky mainland beaches than on steeply sloping island shores. Phyllospadix abundance was positively correlated with P. interruptus abundance; however, at Santa Catalina Island, the Phyllospadix habitat was not extensive enough to be the sole lobster nursery. In laboratory tests, puerulus larvae and early juveniles chose Phyllospadix over rubble rocks or broad-bladed kelp, but did not consistently prefer Phyllospadix over reticulate algae. Ecology, growth, and behavior of juvenile P. interruptus inhabiting a discrete Phyllospadix habitat at Bird Rock, Santa Catalina Island, were investigated from October 1974 through December 1976 by means of frequent scuba surveys. Pueruli settled from June to November. Peak recruitment occurred from July to September, when seasonal temperatures were maximal. Settled larvae were approximately one year old. Juvenile growth was determined by size-frequency, single molt increment, mark-recapture, and laboratory culture studies. Carapace length vs. wet weight relationships fit standard power curve equations. Bird Rock juveniles grew from 7 to 32mm CL in 10-11 molts and from 32 to 56mm CL in 5-6 molts during their first and second benthic years, respectively. Growth rates were similar for males and females. Juveniles regenerating more than two limbs grew less per molt than intact lobsters. Long-term growth of laboratory-reared juveniles was 20% less than that of field lobsters. Growth component multiple regression analyses demonstrated that molt increment was directly proportional to premolt size and temperature for age 1+ lobsters. Molt frequency was inversely proportional to size and directly proportional to temperature. Temperature affected age 2+ lobsters similarly, but molt increment was independent of size, and molt frequency declined at a different rate. Juvenile growth rates more than doubled during warm water months compared to cold water months, primarily because of increased molt frequency. Based on results from this study and from previous investigations, it is estimated that P. interruptus males and females become sexually mature by ages 4 and 5 years, respectively, and that legai size is reached by 7 or 8 years of age. Juvenile P. interruptus activity patterns and foraging behavior were similar to those of adults, except that juvenile home ranges were proportionally smaller, and small juveniles were apparently not attracted to distant food. Small mollusks, abundant in Phyllospadix habitats, were the major food items. Size-dependent predation by fish and octopus apparently caused the considerable juvenile mortality observed at Bird Rock. Juveniles approaching 2 years of age gathered in mixed size-class aggregations by day and foraged beyond the grass beds at night. In autumn, these juveniles migrated to deeper habitats, coincident with new puerulus settlement in the Phyllospadix beds. Based on strong inferences from the results, it is proposed that size-dependent predation is the most important factor determining the !ife history strategy of juvenile P. interruptus. Life history tactics promoting rapid growth apparently function dually in reducing the period of high vulnerability to predation and decreasing the time required to reach sexual maturity. The Phyllospadix habitat is an excellent lobster nursery because it provides shelter from predators and possesses abundant food resources for sustaining optimum juvenile growth rates in shallow, warm water.

Atmospheric carbon dioxide and the climatic record

This paper is an attempt to provide a summary review of conclusions from previous studies on this subject. They have been organized under the following subject headings: Conceptualization of the greenhouse effect; The climatic effect of doubled carbon dioxide; Interpretation of the climatic record; Diagnosis of apparent and possible model deficiencies; The paleoclimatic record.

A study on the effect of Zataria multiflora Bioss essential oil and Nisin on the growth of Lactococcus garvieae in the rainbow trout fillet (Oncorhynchus mykiss)

The ever-increasing population of the world and the growing need for animal protein has doubled the modern man’s demand for food. Additionally, the improvement in the general public health, and the worsening of environmental/ecological pollution have prompted today’s world to look for ways to procure healthy food. And one such attempt is the use of natural preservatives to decrease the bacterial load in foodstuffs, in other words, to increase their durability. This study evaluates the effects of different concentrations of Zataria multiflora Bioss (EO 0, 0.005, 0.015, 0.045, 0.135, 0.405%) and Nisin (0, 0.25, 0.5, 0.75 μg/ml) and storage time (9 days) on the growth of Lactococcus garvieae Ir-170A(856bp) alone, and their combination in a food model system (fillets of the rainbow trout (Oncorhynchus mykiss). Additionally, the growth of a sample of this bacteria in laboratory conditions was studied. The results of this study showed that different concentrations of Nisin had a significant impact (p<0.05) on Lactococcus garvieae. With the value of t in 0.75 μg/ml, the effectiveness rose to 65.77%; the biggest effect on Lactococcus garvieae. And the effect at 4 0C exceeded 80C. The study has also demonstrated that all concentrations of Zataria multiflora Bioss were effective against Lactococcus garvieae. However, with the value of t at 0.405%, the effectiveness was 71.91%. This value had the biggest effect on Lactococcus garvieae. At 4 0C, the effect surpassed the one at 80C. The synergistic effects of the EO and Nisin showed that with the value of t at 0.405% EO and 0.75 μg/ml Nisin was 14.62% had the greatest effect on Lactococcus garvieae. In this study, multi-factorial effects for different concentrations of Zataria multiflora Bioss (EO 0, 0.005, 0.015, 0.0025%), three different concentrations of 122 Nisin (0, 0.25,0.75 μg/ml) and two different levels of PH (5.5 , 7) at two incubation temperatures (15,37) on logp% of Lactococcus garvieae during 43 days in BHI broth were evaluated. Most of the effects on Lactococcus garvieae occurred in PH 5.5 and at a temperature of 150C.