Modulation of the effectiveness of 17-alpha-hydroxy-20-beta-dihydroprogesterone or of a gonadotrophic extract on the in vitro intrafollicular maturation of oocytes of the rainbow trout Salmo gairdnerii by various non-maturing steroids [Translation from: Compte Rendu Hebdomadaire des Seances de l'Academie des Sciences, Paris, Series D 281, 811-814, 1975]

The effectiveness of 17 α-hydroxy-20 β-dihydroprogesterone (17 α-20 β Pg) or of a trout hypophyseal gonadotrophic extract on the in vitro intrafollicular maturation of trout oocytes can be modulated by steroids which do not have a direct maturing effect; the effectiveness of the gonadotrophic extract is lowered by oestradiol and oestrone and increased by testosterone. As these steroids have no significant effect on maturation induced by 17 α-20 β Pg, the site of their activity is probably in the follicular envelopes. Corticosteroids, and Cortisol and cortisone in particular increase the effectiveness of the gonadotrophic extract, but increase the effectiveness of 17 α-20 β Pg even more strongly, suggesting that this 'progestagen' has a direct effect on oocyte sensitivity.

Immunomodulatory effects of domoic acid differ between In vivo and In vitro exposure in mice

The immunotoxic potential of domoic acid (DA), a well-characterized neurotoxin, has not been fully investigated. Phagocytosis and lymphocyte proliferation were evaluated following in vitro and in vivo exposure to assay direct vs indirect effects. Mice were injected intraperitoneally with a single dose of DA (2.5 µg/g b.w.) and sampled after 12, 24, or 48 hr. In a separate experiment, leukocytes and splenocytes were exposed in vitro to 0, 1, 10, or 100 µM DA. In vivo exposure resulted in a significant increase in monocyte phagocytosis (12-hr), a significant decrease in neutrophil phagocytosis (24-hr), a significant decrease in monocyte phagocytosis (48-hr), and a significant reduction in T-cell mitogen-induced lymphocyte proliferation (24-hr). In vitro exposure significantly reduced neutrophil and monocyte phagocytosis at 1 µM. B- and T-cell mitogen-induced lymphocyte proliferation were both significantly increased at 1 and 10 µM, and significantly decreased at 100 µM. Differences between in vitro and in vivo results suggest that DA may exert its immunotoxic effects both directly and indirectly. Modulation of cytosolic calcium suggests that DA exerts its effects through ionotropic glutamate subtype surface receptors at least on monocytes. This study is the first to identify DA as an immunotoxic chemical in a mammalian species.

The role of direct observation in predicting climatic change

The National Oceanic and Atmospheric Administration Center for Ocean Analysis and Prediction (COAP) in Monterey, California, has assembled information to suggest how NOAA's facilities for observing the ocean and atmosphere might be applied to studies of paleoclimate. This effort resulted, indirectly, in several projects that combine direct observations of the ocean/atmosphere system with studies of past climate of the Pacific region. This article considers concepts that link the two kinds of investigations. It defines the thesis that direct observation of systems that generate paleoclimatic information is the nexus upon which understanding of climatic variability begins and upon which prediction of climate and global change depends.

Eastern tropical Pacific corals monitor low latitude climate of the past 400 years

EXTRACT (SEE PDF FOR FULL ABSTRACT): We have measured coral growth band thickness and skeletal stable isotopic composition through a 371-year transect (AD 1583-1954) from a massive specimen of Pavona clavus from the Galápagos Islands. ... We observe a general cooling trend during 1860-1954, corresponding to the end of the Little Ice Age, an interval characterized by general warming at many mid-latitude sites. Variance at sunspot cycle frequencies in growth rate, stable isotopic, and trace element composition implies a direct or indirect link between the solar cycle and climate modulation in the eastern Pacific.

Evidence from the Pacific Northwest for solar modulation of climate and of northern ecosystems

EXTRACT (SEE PDF FOR FULL ABSTRACT): The 250-year net annual snow accumulation, or mass balance, time series derived from the Mt. Logan (Yukon) ice core has been spectrally analyzed and is found to contain a nominal 11-year waveform. The stable isotope time series contains a significant amount of power between 9 and 13 years, although this record is evidently not a straightforward proxy for air temperatures. The signal in the mass balance time series exhibits a close relationship with the sunspot cycle waveform and is, therefore, assumed to be related to it. Waveforms showing a high correlation with the solar cycle are found in other climate data in the region. ... Taken collectively, the data point to a link between solar variability, atmospheric variability, climate, and selected ecological dynamics in the Pacific Northwest, but other data, not presented, indicate these relationships may hold elsewhere. So far, the evidence is empirical; complete details of the physical mechanisms involved have yet to be synthesized in a satisfactory way.

Development of an electric shrimp trawl 1. Reaction of shrimps to low volt direct current

Preliminary attempts were made to assess the effect of direct current on shrimps and to see whether the shrimp could be guided in large numbers into the fishing net by using a current of appropriate voltage without scattering them away as it happens at present. This communication is the first in the series of studies and primarily deals with laboratory equipment and experimental procedures followed.