Genetic and morphological differences between Sebastes vulpes and S. zonatus (Teleostei: Scorpaeniformes: Scorpaenidae)

The taxonomic status of Sebastes vulpes and S. zonatus were clarified by comprehensive genetic (amplif ied fragment length polymorphisms [AFLP] and mitochondrial DNA [mtDNA] variation) and morphological analyses on a total of 65 specimens collected from a single locality. A principal coordinate analysis based on 364 AFLP loci separated the specimens completely into two genetically distinct groups that corresponded to S. vulpes and S. zonatus according to body coloration and that indicated that they are reproductively isolated species. Significant morphological differences were also evident between the two groups; 1) separation by principal component analysis based on 31 measurements, and 2)separation according to differences in counts of gill rakers and dorsal-fin spines without basal scales, and in the frequencies of specimens with small scales on the lower jaw. Restriction of gene flow between the two groups was also indicated by the pairwise ΦST values estimated from variations in partial sequences from the mtDNA control region, although the minimum spanning network did not result in separation into distinct clades. The latter was likely due to incomplete lineage sorting between S. vulpes and S. zonatus owing to their recent speciation.

Atlantic-Mediterranean and within-Mediterranean molecular variation in Coris julis (L. 1758) (Teleostei, Labridae)

Sequence variation in the mitochondrial control region was studied in the Mediterranean rainbow wrasse (Coris julis), a species with pronounced pelagic larval phase inhabiting the Mediterranean Sea and the adjacent coastal eastern Atlantic Ocean. A total of 309 specimens from 19 sampling sites were analysed with the aim of elucidating patterns of molecular variation between the Atlantic and the Mediterranean as well as within the Mediterranean Sea. Phylogeographic analyses revealed a pronounced structuring into a Mediterranean and an Atlantic group. Samples from a site at the Moroccan Mediterranean coast in the Alboran Sea showed intermediate frequencies of “Mediterranean” and “Atlantic” haplotypes. We recognised a departure from molecular neutrality and a star-like genealogy for samples from the Mediterranean Sea, which we propose to have happened due to a recent demographic expansion. The results are discussed in the light of previous studies on molecular variation in fish species between the Atlantic and the Mediterranean and within the Mediterranean.

Population genetic of three species of Clupeidae family Sardinella sindensis, Sardinella albella, Dussumieria acuta in the Persian Gulf & Oman Sea

Sardines and other Microfilidae have very important ecological role in marine ecosystems because they are first consumers in marine food chain and they are the main food of valuable species as tuna. So decries in their population will decline fishing of these spices. There are 10 genus of Clupeidae in south of Iran and Sardinella is the one of the most abundant of them. In this study we investigated about morphological and genetically differences in population of 3 species: Sardinella sindensis, Sardinella abella, Dussomieria acuta. About 65 specimens of Sardinella sindensis, 61 specimens of Sardinella albella and 63 specimens of, Dussomieria acuta from three regions of their distribution: Jask (Oman Sea), Qeshm (Hormoz) and Lengeh (Pearsian Gulf) have been collected. Morphological research of their characters and statistical studies were done. To determine the genetically structure of specie's population we sequenced 500 bp of mitochondrial control region. Genetical studies determine meaningful difference in alleles and heterozigosity frequency of Sardinella sindensis. This must be the result of divergence in population of this species. Morphological investigation of Sardinella albella shows the meaningful difference. But detailed studies diffused it. Genetical studies show a meaningful variance in allele and heterosigosity frequency. This may be an aspect of sardine tendency to live in estuaries. Morphological research of Dussomieria acuta in Jask and Lengeh show a meaningful variance in these regions. Such a situation might be result of Monsoon, upwelling and better weather which occur in Oman Sea in spite of Persian Gulf.

Population genetic structure of Neogobius caspius (Eichwald ,1831) in the South Caspian Sea using PCR-RFLP Marker

Neogobius caspius is a small benthic fish that is native to the Caspian Sea. The importance of this fish is because of it is role as a main food resource of the sturgeon fish. The genetic diversity of N. caspius population in the Caspian Sea was studied using PCR- RFLP technique. A total of 135 samples of N. caspius were collected from coastal line in the north Caspian sea, including specimens from coasts of Anzali , Torkman Port and Chalus. Genomic DNA was extracted by phenol-chloroform method and then was amplified using a pair primer of cytochrom b gene, 2 tRNA gene and the control region sequences by a thermal cycler. D2 (5'-CCGGAGTATGTAGGGCATTCTCAC-3'), CY1 (5'-YYTAACCRRGACYAATGACTTGA-3') 12 restriction enzyme were used to digest the target gene region including: Alul HincII —Tas1 —Rsa1 -MboI -DraI -BSeNI(BSRI) Alw261(BsmAI). Bsul 51 Hin11 Bsh12851- BsuRI(HaeIII) digested PCR products were observed by silver staining method followed by Polyacrylamide gel electrophoresis (PAGE). The results were shown the same pattern among the species. There was no polymorphism and no differentiation in population in the Neogobius caspius fish and all individuals have shown homogenous genotype.

The water hyacinth problem and the biological control option in the highland lake region of the upper Nile basin: Uganda's experience

The rapid proliferation and extensive spread of water hyacinth Eichhornia crassipes (Mart) Solms in the highland lakes of the Nile Basin within less than 15 years of introduction into the basin in the 1980s pauses potential environmental and social economic menace if the noxious weed is not controlled soon. The water weed has spread all round Lake Victoria and, in Uganda where infes tation is mos t severe, water hyacinth estimated at 1,330,000 ton smothers over 2,000 ha of the lakeshore (August,1994). Lake Kyoga which already constantly supplies River Nile with the weed is infested with over 570 ha, while over 80% of the river course in Uganda is fringed on either side with an average width of about 5m of water hyacinth. As the impact of infestation with water hyacinth on water quality and availability, transportation by water, fishing activities, fisheries ecology, hydro-power generation etc becomes clear in Uganda, serious discussion is under way on how to control and manage the noxious weed. This paper pauses some of the questions being asked regarding the possible application of mechanical and chemical means to control the water weed.Uganda has already initiated the use of biological control of water hyacinth on Lake Kyoga with a strategy to use two weevils namely Neochetinabruchi and Neochetina eichhorniae. The strategy to build capacity and infrastructure for mass multiplication and deployment of biological control of the weevils in the field developed in Uganda by the Fisheries Research Insti tu te (FIRI) and the Namulonge Agricultural and Animal production Research Insti tute (NAARI) is proposed in outline for evaluation. Plans to deploy this strategy on lake Kyoga are under way

Tilapia for Biological Control of Giant Salvinia

In August 1999, giant salvinia ( Salvinia molesta Mitchell) was found along the lower Colorado River in irrigation drainages. To investigate the slow spread and apparent control of giant salvinia in this region, the herbivorous fish, tilapia (Oreochromis niloticus Trewavas), was examined as a biological control agent. The study was conducted in a 5,000-L recirculating system. (PDF contains 4 pages.)

Mechanical control of floating aquatic weed: Kainji Lake experience

The paper describes the uniqueness and invasiveness of water hyacinth (Eichhornia crassipes) on Lake Kainji (Nigeria). The mechanical blocking device design concept based on the Kainji Lake flooding regime is also highlighted. Water hyacinth coverage, that was over 23% at high water in level in 1994, was reduced to 0.75% in the same period in 2000. Although this feat cannot be wholly ascribed to mechanical control effort alone, the first year of the device's full operation more than 1.04 million kg of fresh weight of water hyacinth were trapped, collected and deposited in two separate dumping pits, each at about 1 km off the shoreline of either side of the Lake. On further analysis over a period of one year of uncleared inflow of water hyacinth indicated the effectiveness of the bloom. Recommendations are advanced for the use of such local but highly technical knowledge to control floating water hyacinth that is vastly taking over the intricate network of Nigerian water systems and within the West African sub-region

South Pennine Moors cSAC-SPA. North West Region-South Area

This is the report on Habitats Directive, the Review of Consents Stage 1 and 2 by the Environment Agency of the South Pennine Moors cSAC/SPA. The Habitats Directive has the main aim to promote the maintenance of biodiversity by defining a common framework for the conservation of wild plants and animals and habitats of community interest. The Directive establishes a European ecological network known as "Natura 2000". The network comprises Special Areas of Conservation (SAC) and Special Protection Areas (SPA). In the section on Stage 1 or Screening Process of the Habitat Directive, it is identified the likely impacts upon the Rixton Clay Pits cSAC of a project, plan or activities, either alone or in combination with other projects, plans or activities, and considers whether these impacts are likely to be significant. In the section on Stage 2 or Appropiate Assessment of the Habitat Directive, it is considered the impact on the integrity of the Rixton Clay Pits cSAC of the projects, plans or activities, either alone or in combination with other projects, plans or activities, with respect to the site’s structure and function and its conservation objectives. Additionally, where these are adverse impacts, an assessment of the potential mitigation of those impacts. The criteria used in this report to identify relevant projects, plans or activities and their impacts are water quality discharge consents, waste management licences, abstraction licences, Integration Pollution Control (IPC) and Integrated Pollution Prevention Control (IPPC) permits. Proformas, hydrogeological and GIS maps are included in the review.

Impacts of fish sanctuaries on the production and diversity of plankton on beels of haor region in Bangladesh

The experiment was carried out to study the impacts of fish sanctuaries on the production and diversity of plankton in beels of haor region at Mithamain Upazila of Kishoreganj district in Bangladesh during July 2004 to June 2005. A total of 75 (60 phyto and 15 zooplankton) and 74 (59 phyto and 15 zooplankton) genera of plankton were recorded in T-1 and T-2 (with sanctuary) respectively while only 50 (39 phyto and 11 zooplankton) genera were obtained in T-3 (control). Chlorophyceae and Copepoda were the most dominant group of phytoplankton and zooplankton respectively in all the treatments. The total phytoplankton numbers were found to range from 5472 to 35,833 cells/l and 5250 to 40,472 cells/l and total zooplankton from 667 to 1722 cells/l and 611 to 1667 cells/l in T-1 and T-2 respectively in sanctuary sites whereas the ranges of phytoplankton and zooplankton in the control site were 1778 to 29,333 cells/l and 56 to 1056 cells/l respectively. The maximum phytoplankton and zooplankton were recorded during winter season in all the treatments. The ranges of total plankton were 6194 to 37,500 cells/l, 6028 to 41,806 cells/l and 1889 to 29,444 cells/l in T-1, T-2 and T-3 respectively. The phytoplankton, zooplankton and total plankton recorded in treatments with sanctuary were significantly higher (p<0.5) than the treatment without sanctuary (control) indicating positive impacts of sanctuaries on the production of plankton. Between two treatments of fish sanctuaries the total plankton populations were comparatively higher in T-2 than T-1.

Observations on the biology of Nothobranchius guentheri (Pfeffer) (Cyprinodontidae), an annual fish from the coastal region of East Africa

Nothobranchius guntheri is found in seasonal pools and streams in the coastal region of Tanzania. A population recurring annually in a pond near Kilosa has been studied. Growth in length was rapid and maximum mean lengths were attained within 11-12 and 7-8 weeks of hatching by males and females respectively. Males grew larger and exhibited wider variation in length than females. N. guentheri shows clear sexual dichromatism. No significant inequality in the sex ratio was found. Females with ripe eggs were found 7-8 weeks after hatching. Spawning continued throughout adult life and fecundity increased markedly with increasing length. In laboratory aquaria, aggressiveness between adult males was noted and females were actively driven on to the substratum preparatory to spawning. The diet of the fish pond consisted chiefly of aquatic and terrestrial insects, of which midge larvae and pupae were the most common. N. guentheri is exploited by man in the aquarist trade and for the biological control of mosquitoes. An extended redescription of the species is appended which includes N. melanospilus (Pfeffer) as a synonym.