Simulations of phytoplankton dynamics in El Gergal Reservoir, Southern Spain (PROTECH)

The Mediterranean region is characterised by a variable climate with most of the rain falling during the winter and frequent summer droughts. Such warm, dry periods are ideal for the growth of large algal blooms that often consist of potentially toxic Cyanobacteria. This makes the management of water for human use particularly challenging in such a climate and it is important to understand how such blooms can be avoided or at least be reduced in size. PROTECH (Phytoplankton RespOnses To Environmental CHange) is a model that simulates the dynamics of different species of phytoplankton populations in lakes and reservoirs. Its distinct advantage over similar models is its ability to simulate the relative composition of the algal flora, allowing both quantitative and qualitative conclusions to be drawn e.g. whether Cyanobacteria could be a potential problem. PROTECH has been applied primarily to lakes and reservoirs in northern Europe. Recently, however, the model has been applied to water bodies in lower latitudes, including Australia to a water supply reservoir in the south of Spain, El Gergal. El Gergal is the last in a chain of reservoirs that supply water to the city of Seville. It was brought into service in April 1979 and has a maximum storage volume of 35 000 000 m3. This article summarises the application of PROTECH in order to simulate the following problems: • the effect of a large influx of Ceratium biomass into El Gergal from another reservoir • the effect of using alternative water sources instead of the Guadalquivir River (used occasionally to raise water levels in El Gergal) • the effect of installing tertiary sewage treatment on the Cala River • the effect of simulated drought conditions on phytoplankton in the reservoir.

Assessment Method for Leaf Litters Allelopathic Effect on Cyanobacteria

A new bioassay technique combining leaf disk and softagar over-layer methods was developed to investigate the allelopathic effect of deciduous leaf litters on the growth of cyanobacteria ( Microcystis aeruginosa Kütz.). Bioactive substances exuded from leaf disks caused inhibitory plaques on the agar plate containing cyanobacteria , and the rate of diffusion depended on the specific leaf disk area. Most of the leaf litters collected around reservoirs in Japan showed inhibitory activity to M. aeruginosa , with Rhus trichocarpa Miq., Quercus variabilis Blume and Mallotus japonicus (Thunb.) Muell. Arg. being the strongest among the 22 tested species.(PDF has 4 pages.)

Evaluation of Several Commercial Algicides for Control of Odor-producing Cyanobacteria

The production of certain odorous metabolites is an undesirable attribute of cyanobacteria (blue-green algae) growth in aquaculture ponds [e.g., channel catfish(Ictalurus punctatus)] and in drinking water reservoirs. The most common odorous compounds encountered in catfish aquaculture are geosmin (trans-1,10-dimethyltrans-9-decalol) and 2-methylisoborneol(exo-1,2,7,7-tetramethylbicyclo[2.2.1]heptan-2-ol). These compounds are also frequently encountered worldwide in reservoirs and aqueducts used for municipal drinking water systems(Schrader et al. 2002). In this study, several algicides were evaluated using a rapid bioassay to determine their effectiveness in controlling the MIB-producing cyanobacterium Oscillatoria perornata from a west Mississippi catfish pond and the MIBproducing Pseudanabaena sp. (strain LW397) from Lake Whitehurst, Virginia, used as a city water supply reservoir. The cyanobacterium Oscillatoria agardhii , not a MIB-producer, and the green alga Selenastrum capricornutum , found in catfish ponds in the southeastern United States, were included in the bioassay to help determine potential broad-spectrum toxicity of the commercial products. (PDF has 3 pages.)

Toxic pollutants, health indices and population dynamics of Harbor Seals in San Francisco Bay, 1989-1992

(PDF contains 300 pages)

Toxic effect of Raphia vinifera on fish leech (Piscicola geometra)

This study examines acute toxicity of Raphia vinifera on fish leech, Piscicola geometra. The leeches with a mean total length of (TL) 4.2+1.0cm were exposed to various concentrations of both crude powdered and ethanolic extracts of the botanical. Median lethal concentration (LC50) was determined with static-renewal tests using logarithmic and arithmetic graphic methods. The LC50 (for 96 hours of crude powdered (aqueous) extracts of the botanical on Piscicola geometra was 1.10 ppm arithmetically and 1.14ppm logarithmically. The 95% confidence limits was 0.10ppm arithmetically and 0.12ppm logarithmically. The LC50 of ethanolic extract of the poison at 96-h was 0.5ppm arithmetically and 0.48ppm logarithmically. The 95% confidence limits were less than 0.10ppm. The use of extracts of R. vinifera in the control of leeches in fish ponds is discussed

Toxic blue-green algae: The "problem" in perspective

The incidence of blue-green algal blooms and surface scum-formation are certainly not new phenomena. Many British and European authors have been faithfully describing the unmistakable symptoms of blue-green algal scums for over 800 years. There is no disputing that blue-green algal toxins are extremely harmful. Three quite separate categories of compound have been separated: neurotoxins; hepatotoxins and lipopolysaccharides. There is a popular association between blue-green algae and eutrophication. Certainly the main nuisance species - of Microcystis, Anabaena and Aphanizomenon are rare in oligotrophic lakes and reservoirs. Several approaches have been proposed for the control of blue-green algae. Distinction is made between methods for discharging algae already present (eg algicides; straw bales; viruses; parasitic fungi and herbivorous ciliates), and methods for averting an anticipated abundance in the future (phosphorous control, artificial circulation etc).

The meaning of some common terms used in sampling toxic phytoplankton

The author explains some aspects of sampling phytoplankton blooms and the evaluation of results obtained from different methods. Qualitative and quantitative sampling is covered as well as filtration, freeze-drying and toxin separation.

Taking a systems-oriented view of phosphorous enrichment in fresh waters

Eutrophication of fresh waters through anthropogenic enrichment by phosphorus is a global problem. The role of phosphorus enrichment in the formation of blooms of toxic blue-green algae (Cyanobacteria) in fresh waters is well established and of considerable concern in terms of human and animal health, loss of water resources and amenities, threats to fish stocks, and aesthetic considerations. Cultural eutrophication also poses threats to the ecosystem balance in fresh waters, with implications for wildlife. This article examines phosphorus enrichment in fresh waters from a systems perspective, and explores systems solutions that may be helpful in the development of more sustainable policies.

A decade of monitoring and management of freshwater algae, in particular Cyanobacteria, in England and Wales

Although the toxicity of cyanobacteria has been known for many years, cyanobacteria-related problems in the UK were generally limited in frequency. However, this all changed and became of national concern following the exceptional environmental conditions in the autumn of 1989, when widespread cyanobacterial blooms and scums developed in fresh waters. This paper summarises the Environment Agency's monitoring programme for freshwater algae since 1991 and describes the actions the Environment Agency has taken as a result of the monitoring data.

A control of gas-vacuolate cyanobacteria

The cyanobacteria that cause problems in water supply are principally the colonial forms that are buoyed up by gas vesicles. The success of these organisms is due, in part, to their gas vesicles, which enable them to perform vertical migrations or to maintain themselves in the euphotic zone. The gas vesicles are also the root cause of the problems. In calm periods they cause the cyanobacteria to float to the water surface forming noxious scums, and they may prevent the colonies from sedimenting in water treatment plants. Gas vesicles are hollow, gas-filled structures; they are rigid but can be collapsed by the application of pressure. Their critical collapse pressure is influenced by their dimensions, which vary in different organisms. Gas vesicles are formed by the assembly of two types of protein, which determine their mechanical and physical properties. Methods for collapsing gas vesicles in natural populations of cyanobacteria will be considered. They may have application to the control of cyanobacteria in water supply.

Molecular biomarkers and toxic consequences of impact by organic pollution in aquatic organisms

Organic contaminants are readily bioaccumulated by aquatic organisms. Exposure to and toxic effects of contaminants can be measured in terms of the biochemical responses of the organisms (i.e. molecular biomarkers). The hepatic biotransformation enzyme cytochrome P4501A (CYP1A) in vertebrates is specifically induced by organic contaminants such as aromatic hydrocarbons, PCBs and dioxins, and is involved in chemical carcinogenesis via catalysis of the covalent binding of organic contaminants to DNA (DNA-adducts). Hepatic CYP1A induction has been used extensively and successfully as a biomarker of organic contaminant exposure in fish. Fewer but equally encouraging studies in fish have used hepatic bulky, hydrophobic DNA-adducts as biomarkers of organic contaminant damage. Much less is known of the situation in marine invertebrates, but a CYPlA-like enzyme with limited inducibility and some potential for biomarker application is indicated. Stimulation of reactive oxygen species (ROS) production is another potential mechanism of organic contaminant-mediated DNA and other damage in aquatic organisms. A combination of antioxidant (enzymes, scavengers) and pro-oxidant (oxidised DNA bases, lipid peroxidation) measurements may have potential as a biomarker of organic contaminant exposure (particularly those chemicals which do not induce CYP1A) and/or oxidative stress, but more studies are required. Both CYP1A- and ROS-mediated toxicity are indicated to result in higher order deleterious effects, including cancer and other aspects of animal fitness.

Characterization of toxic impacts on living marine resources in tidal rivers of the Chesapeake Bay

In 1999, the Chesapeake Bay Program completed a survey of existing data on chemical contaminants and the potential for bioeffects in 38 tidal river systems of Chesapeake Bay. This review led to the identification of 20 areas for which there were insufficient data to adequately characterize the potential for contaminant bioeffects on the Bay’s living resources. The goal of the present study was to estimate the current status of ecological condition in five of these areas and thus help to complete the overall toxics inventory for the Bay. These five systems included the Chester River, Nanticoke River, Pocomoke River, Lower Mobjack Bay (Poquosin and Back Rivers) and the South and Rhode Rivers. This study utilized a Sediment Quality Triad (SQT) approach in combination with additional water-column contaminant analysis to allow for a “weight of evidence” assessment of environmental condition. A total of 60 stations distributed among the five systems, using a probabilistic stratified random design, were sampled during the summer of 2004 to allow for synoptic measures of sediment contamination, sediment toxicity, and benthic condition. Upon completion of all analyses, stations were assigned to one of four categories based on the three legs of the triad. Stations with high sediment quality had no hits on any of the three legs of the triad; those with moderate quality had one hit; those with marginal quality had two hits; and those with poor quality had hits for all three legs of the triad. The Pocomoke River had by far the largest proportion of the total area (97.5%) classified as having high sediment quality, while the Rhode/South system had the highest proportion (11.4%) classified as poor. None of the stations in the Chester River, Nanticoke River, and Lower Mobjack Bay systems were classified as poor. More than 65% of the area of each of the five systems was classified with high to moderate sediment quality. The Rhode/South system had 30.4% of total area classified with marginally to severely poor quality. The results of this study highlight the importance of using multiple indicators and a “weight of evidence” approach to characterize environmental quality and the potential bioeffects of toxic contaminants.

Global transcriptional responses of the toxic cyanobacterium, Microcystis aeruginosa, to nitrogen stress, phosphorus stress, and growth on organic matter

Whole transcriptome shotgun sequencing (RNA-seq) was used to assess the transcriptomic response of the toxic cyanobacterium Microcystis aeruginosa during growth with low levels of dissolved inorganic nitrogen (low N), low levels of dissolved inorganic phosphorus (low P), and in the presence of high levels of high molecular weight dissolved organic matter (HMWDOM). Under low N, one third of the genome was differentially expressed, with significant increases in transcripts observed among genes within the nir operon, urea transport genes (urtBCDE), and amino acid transporters while significant decreases in transcripts were observed in genes related to photosynthesis. There was also a significant decrease in the transcription of the microcystin synthetase gene set under low N and a significant decrease in microcystin content per Microcystis cell demonstrating that N supply influences cellular toxicity. Under low P, 27% of the genome was differentially expressed. The Pho regulon was induced leading to large increases in transcript levels of the alkaline phosphatase phoX, the Pst transport system (pstABC), and the sphX gene, and transcripts of multiple sulfate transporter were also significantly more abundant. While the transcriptional response to growth on HMWDOM was smaller (5–22% of genes differentially expressed), transcripts of multiple genes specifically associated with the transport and degradation of organic compounds were significantly more abundant within HMWDOM treatments and thus may be recruited by Microcystis to utilize these substrates. Collectively, these findings provide a comprehensive understanding of the nutritional physiology of this toxic, bloom-forming cyanobacterium and the role of N in controlling microcystin synthesis.