Detection of cryptosporidium oocysts in water and environmental concentrates

Whilst current methods for the isolation and enumeration of Cryptosporidium spp. oocysts in water have provided some insight into their occurrence and significance, they are regarded as being inefficient, variable and time-consuming, with much of the interpretation being left to the expertise of the analyst. Two expectations of novel developments are to reduce the variability and subjectivity associated with the isolation and identification of oocysts. Flocculation, immunomagnetisable and flow cytometric techniques, for concentrating oocysts from water samples, should prove more reliable than current methods, whilst the development of more avid and specific monoclonal antibodies in conjunction with the use of nuclear fluorochromes will aid identification. Further insight into the viability, taxonomy, species identification, infectivity and virulence of the parasite should be forthcoming through the use of techniques such as the polymerase chain reaction, in situ hybridisation and non-uniform alternating current electrical fields. Such information is necessary in order to enable microbiologists, epidemiologists, engineers, utility operators and regulators to assess the safety of a water supply, with respect to Cryptosporidium contamination, more effectively.

Escala macroscópica de maturaçāo e ciclo sexual das espécies Penaeus indicus H. Milne Edward e Metapenaeus monoceros (Fabricius)

The macroscopic scale used for determination of sexual maturity in shrimps was tested against objective parameters namely the gonad-somatic index and the size of oocytes. The two main species caught in Mozambique, Penaeus indicus and Metapenaeus monoceros, were the object of the work. In order to reduce the subjectivity in the macroscopic exam, a table with the colours representative of each stage is presented. Since this study was conducted over a period of 13 consecutive months and there were observations from previous years, the sexual cycle of the two species is also presented, as well as an estimate of the size at first maturity.