Aquaculture Asia, Vol.13, No.4, pp.1-51, October-December 2008

Editorial An increasingly secure future for wastewater-fed aquaculture in Kolkata, India? by Peter Edwards. First culture-based fisheries growth cycle in Lao PDR is overwhelmingly encouraging, by Sena De Silva. Revival of abandoned shrimp farms in Krishna District of Andhra Pradesh, by National Centre for Sustainable Aquaculture (NaCSA). Growth of forward and backward industries linked with aquaculture in Kolleru Lake area, Andhra Pradesh, India, by A. K. Roy, G. S. Saha, P. Kumaraiah and N. Sarangi Effective marketing strategies for economic viability of prawn farming in Kuttanad, India, by Ranjeet K. and B. Madhusoodana Kurup. Applications of nutritional biotechnology in aquaculture, by S.D. Singh, S.K. Nayak, M. Sekar and B.K. Behera. Some technical and management aspects of catfish hatcheries in Hong Ngu district, Dong Thap province, Vietnam, by H. P. Hung, N. T. T. An, N. V. Trieu, D. T. Yen, U. Na-Nakorn, Thuy T. T. Nguyen. Nodavirus: An emerging threat to freshwater prawn farming, by Biju Sam Kamalam, J., Saravanan, S. and Ajith Stalin, J.L. Asia-Pacific Marine Finfish Aquaculture Network Magazine: Asian seabass farming: Brainstorming workshop and training in India. Comparative study for broodstock management of grey mullet (Mugil cephalus L.) in cages and earthen ponds with hormone treatment, by Nani Gopal Das, Md. Shahadat Hossain, Sushanta Bhattacharjee and Prabal Barua. Cultivation of gilthead sea bream (Sparus auratus L.) in low saline inland water of the southern part of Israel desert, by Samuel Appelbaum and A. Jesu Arockia Raj. Mariculture development opportunities in SE Sulawesi, Indonesia, by La Ode M. Aslan, Hotman Hutauruk, Armen Zulham,Irwan Effendy, Mhummaed Atid, Michael Phillips, Lars Olsen, Brendan Larkin, Sena S De Silva, Geoff Gooley. Improved hatchery and grow-out technology for marine finfish. NACA Newsletter

The use of the ethanol pathway in goldfish Carassius auratus (L.) following anoxia

Goldfish (Carassius auratus) were subjected, for a period of 6 weeks, to 2h progressive hypoxia followed by 6h anoxia in closed respirometers at 15 degree C. The concentrations of glucose, lactate and ethanol were determined in whole goldfish following exposure to both hypoxia and anoxia. Lactate accumulation (mmol/kg/h) was 0.35 during the 1st week but declined to 0.14 in the 6th week of exposure to anoxia. In contrast, ethanol excreted to the surrounding water, increased from 65% to 92% of the total production in the lst and 6th week, respectively. The switch from lactate accumulation to ethanol pathway utilization, with the resultant metabolic depression and anoxia resistance is discussed

Probiotics and nutrient supplement in artificial feed of gold fish (Carassius auratus)

The efficacy of three feeds incorporated with different probiotics -Lactobacil, Vizylac and Cyfolac as nutrient supplement was evaluated in an ornamental fish, Carassius auratus (Linn.). The basal diet (40% protein) was prepared and the probiotics were incorporated at different levels viz. Lactobacil at 8x10 super(7)/100g and 12x10 super(7)/100g, Vizylac at 8x10 super(7)/100g and 12x10 super(7)/100g and Cyfolac at 12x10 super(7)/100g. Feeding trial was conducted for a period of 4 weeks. At the end of the experiment, bio-growth parameters and proximate composition of the fishes were studied. The growth, survival and protein content were improved in all the probiotic fed fishes compared to control. The maximum growth (0.74 g) and survival (85%) were observed in fishes fed with Lactobacil at 12x10 super(7)/100g. It emphasizes that supplementation of feed with probiotics has a positive impact on the growth, survival and the body composition of goldfish.

Effect of feeding bioencapsulated Lactobacillus sp. in live Tubifex sp. on the growth performance of gold fish Carassius auratus (Linnaeus, 1758)

An attempt was made to feed bioencapsulate Lactobacillus sp. in live fish food organism Tubifex for use in the culture of gold fish Carassius auratus. The C. auratus fries when fed with bioencapsulated Lactobacillus sp. in Tubifex showed significant improvement in total wet weight gain (p<0.007) and FCR (p<0.01) compared to control. The specific growth rale and mean survival were slightly higher, although insignificantly (p>0.05) in bioencapsulated Tubifex fed group. None of the bacteriological parameters of the fish gut between the experimental and control groups differed significantly (p>0.05). Lactobacillus sp. was recorded at a level of log 5.11/g on the 90th day of experimentation. When the experimental C. auratus fries were infected with Pseudomonas fluorescents, the bioencapsulated Tubifex fed group resisted the infection. The survival was significantly higher (p<0.05) in bioencapsulated Tubifex fed group (44%) than in control (22%). The C. auratus fed with bioencapsulated Tubifex showed less (55%) signs of tail/fin rot. Likewise, a significant improvement in total wet weight gain (p<0.009), FCR (p<0.01) and SGR (p<0.04) of C. auratus brooder fed with bioencapsulated Tubifex was seen compared to control group fed with depurated Tubifex.

Untersuchungen zur Qualität von Doraden auf dem deutschen Markt

Mediterranean fish species and fish species from other southern countries are retailed increasingly on the German market. The fish is stored in ice eviscerated or as whole fish and imported by truck or by airplane. In the present investigation, the freshness, the sensory quality and the microbiological status was determined of gilthead sea breams (Sparus aurata) from farms in Italy and France and from sea breams (Sparus ehrenbergi) of wild captures from Senegal. It is shown, that farmed gilthead sea breams were of high sensory quality and in good microbiological conditions.

Survey of fungal infestation of some fish species from Tagwai dam, Minna, Niger State

Survey of Fungal infestation of some species of fish in Tagwai Dam Minna was carried out from March to June 2002. Fungi were isolated from the scale/skin, gills and fins. Twenty-one fungi species were identified from 18 species of fish microbial growth was measured by direct cell count using Stuart colony counter. Most of the fungi encountered were of the mould group and infestation occurred among all the species sampled. The infestation was predominantly by Aspergillus species and the scale/skin was most widely affected. The study showed the identified fungi in order of frequency to be as follows. Aspergillius niger. Rrhizopus spp. Mmucor spp Aspergilus flavum. Aspergillus parasitous. Aspergillus fumigatus, Microsporum canis. Penicillum virridicalumand Fusarium spp respectively. Aspergillus niger occurred on all the species of fish sampled. Barrilius spa and Chrysichrhy auratus longifilis had significantly higher (P<0.05) mean fungal load on their fins and gills. There was no significant difference (P>0.05) in the mean fungal load in different parts of the body of other fish species sampled

Growth performance of Nigerian fish stocks

Parameters of the von Bertalanffy growth function are presented for 42 fish stocks belonging to 16 families, 22 genera and 27 species. The growth performance index, Phi '(= log K + 2logL sub( infinity )), was computed for each stock and was found to be highest in male Gymnarchus niloticus (Gymnarchidae) from Lake Chad and lowest in Chrysichthys auratus (Bagridae) from the Cross River. Mean Phi ' for major fish genera and families are also presented and was highest in brackishwater fishes, closely followed by freshwater and inshore marine water fishes.

A general framework for integrating environmental time series into stock assessment models: model description, simulation testing, and example

We present a method to integrate environmental time series into stock assessment models and to test the significance of correlations between population processes and the environmental time series. Parameters that relate the environmental time series to population processes are included in the stock assessment model, and likelihood ratio tests are used to determine if the parameters improve the fit to the data significantly. Two approaches are considered to integrate the environmental relationship. In the environmental model, the population dynamics process (e.g. recruitment) is proportional to the environmental variable, whereas in the environmental model with process error it is proportional to the environmental variable, but the model allows an additional temporal variation (process error) constrained by a log-normal distribution. The methods are tested by using simulation analysis and compared to the traditional method of correlating model estimates with environmental variables outside the estimation procedure. In the traditional method, the estimates of recruitment were provided by a model that allowed the recruitment only to have a temporal variation constrained by a log-normal distribution. We illustrate the methods by applying them to test the statistical significance of the correlation between sea-surface temperature (SST) and recruitment to the snapper (Pagrus auratus) stock in the Hauraki Gulf–Bay of Plenty, New Zealand. Simulation analyses indicated that the integrated approach with additional process error is superior to the traditional method of correlating model estimates with environmental variables outside the estimation procedure. The results suggest that, for the snapper stock, recruitment is positively correlated with SST at the time of spawning.

Bacterial flora associated with diseased fish and their antibiogram

Bacterial flora associated with tail rot/fin rot of Carassius auratus, Xiphophorus helleri and hemorrhagic ulcers of Clarias spp were studied. Sensitivity pattern of 33 isolates comprising Aeromonas spp, Pseudomonas spp and Gram-positive rods from diseased C. auratus, X. helleri and Clarias spp were screened against six broad-spectrum antibiotics viz. ciprofloxacin, chloramphenicol, co-trimoxazole, gentamycin, nitro-furantoin and oxytetracycline. Ciprofloxacin was the most effective in inhibiting bacteria at 0.05-0.10 µg/ml level. About 44% of Pseudomonas spp. was resistant to nitrofurantoin. Resistance to oxytetracycline was seen in 27% of Aeromonas spp Gram-positive rods were comparatively more resistant to antibiotics. The multiple antibiotic resistances were seen in 21% of the bacterial isolates of diseased fish.

Effects of dietary supplementation of commercial human probiotic and antibiotic on the growth rate and content of intestinal microflora in ornamental fishes

The effects of dietary supplementation of commercial human probiotic, Lactobacil and antibiotic, oxytetracycline on the growth, survival, disease resistance and content of intestinal microflora in two ornamental fishes, viz., goldfish, Carassius auratus and swordtail, Xiphophorus helleri were studied. The total wet weight gain, food conversion ratio and specific growth rate of C. auratus did not vary significantly (p>0.05) among treatments. While in X. helleri, significant differences existed in the total wet weight gain, survival, food conversion ratio and specific growth rate among treatment groups (p<0.05). The counts of antibiotic resistant bacteria in fish gut increased with days of culture in all the treatments and the increase was more in antibiotic fed fishes. A reduction in the development of antibiotic resistance among the bacterial flora of fish gut was noticed in probiotic fed groups of C auratus and X. helleri. The results of the present study revealed that the effects of human probiotic on the growth, survival and disease resistance of ornamental fishes are variable and difficult to reproduce the similar effect on different species.

Mercury bioaccumulation in pike (Esox lucius) food chain from Anzali Lagoon, Iran

A number of wide-ranging monitoring studies have been performed in order to estimate the degree of mercury (Hg) contamination in freshwater ecosystems. Knowledge regarding contamination of different levels of the food chain is necessary for estimation of total pollutant input fluxes and subsequent partitioning among different phases in the aquatic system. The growing international concern about this environmental data is closely related to the strongly developing ecological risk assessment activities. In addition,freshwater monitoring outputs hold a key position in the estimation of the Hg dose consumed by the human population as it is highly dependent on fish consumption. So monitoring of Hg in the tissue of edible fish is extremely important because of contaminated fish has caused serious neurological damage to new born babies and adults. Mercury tends to accumulate in fish tissue, particularly, in the form of methyl mercury, which is about 10 times more toxic than inorganic mercury. The Anzali lagoon is one of the biggest wetland of Guilan province, which joins to the Caspian sea. Many Chemical and industrial factories plus agricultural runoffs and urban and rural sewages are major polluting sources of the Anzali wetland. Since many of those polluting sources drain their wastes directly or indirectly into the Anzali wetland and their sewages may be polluted with Hg, this study was conducted to find out the bioaccumulation of Hg bioaccumulation in pike (Esox lucius) food chain from Anzali lagoon, Iran. Sampling were carried out from July 2004 to July 2005, in addition 318 speciments of 9 fish species were collected. T-Hg was measured by LECO AMA 254 Advanced Mercury Analyzer (USA) according to ASTM standard No D-6722. Each sample was analyzed 3 times. Accuracy of T-Hg analysis was checked by running three samples of Standard Reference Materials; SRM 1633b, SRM 2711 & Sra 2709. Detection limit was 0.001 mg/kg in dry weight. The Accuracy degree of analyzor equipment with RSD<%0.05 (N=7) was between %95.5 and %105. In overal eigth fish species were distingushed in the gut content of 87 speciments of pike with age 1-5 year and maximum length 550mm. The max. and min. concentration of T-Hg in dorsal muscle of pjke was 0.2ppm in one year and 1.2ppm in five year class. The mean of T-Hg significantly increased with age and length increased (P<0.05).Mercury accumulation pattern in pike was as well as muscle > liver > spleen (P<0.05). THg content in female was higher than male(P<0.05). In contrast the mean of THg concentration in dorsal muscle of eigth fish species as prey was 0.282, 0.261, 0.328, 0.254, 0.256, 0.286, 0.322 and 0.241 ppm for Carassius auratus gibelio, Hemiculter leucisculus, Blicca bjoerkna transcaucasica, Chalcalburnus mossulensis, Rhodeus sericeus amarus, Gambusia holbrooki, Alburnus charusini hohenackeri & Scardinius Erythrophthalmus respectively.Liner regresion indicated that high degree of relationship between age of pike and Uptak/Intake ratio (R2=%99.12) and indicated that the mercury bioaccumulation in the pike dorsal muscle increased with age increased. BFA was >1 and and indicating the mercury biomagnification in the pike food chain. Trophy level of pike in the Anzali lagoon was estimated as well as 3.5 and 4 . It is generally agreed that Hg concentration in carnivorous fish are higher than in noncarnivorous species.

Study ectoparasite infestation of 10 common imported freshwater ornamental fish species

In the present study, ectoparasite infestation of common freshwater ornamental fish species imported into Iran including: Dwarf gourami (Colisa lalia), Angelfish (Pterophyllum scalare), Oscar (Astronatus ocellatus), Red eye Tetra ( Moenkhansia Sanctaefilomenae), Barb( Capoeta tetrazona), Arowana (Osteoglossum bicirrhosum), Goldfish(Carassius auratus), Red fin Shark (Lebeo elythrurus, Catfish (Hypostomus plecostomus) and Pangasius sutchi ( Pangasius hypophthalmus) from May until April was investigated.A total of 6 specimens sample of each fish species) randomly were obtained and taken alive to the lab. After observing gill and skin wet smear under the microscope , gill was dissected and examined carefully .Photo and films were taken from the isolated parasites and parasite identification was performed according to Yamaguti AP1), Bychowsky (■Y).From a total of examined fishes 1\ ► sample (YY.V.) were parasitized .Parasite groups that were observed consisting ciliated protozoan myxosporidian , monogenean Digenean metacercaria and crustacean .The only non-parasitized fish was Barb. Monogenea Trianchoratus sp. , Cleidodiscus sp. , Ancylodiscoides sp. , Thaparocleidus sp. , Centrocestus formosanus metacercaria and Myxobolus longisporus report for the first time in ornamental fish which imported from Southeast of Asia to Iran. According to the results, it seems that severe quarantine and sanitary rules are necessary.

Diet seasonality and overlap of Chrysichthys species on Lake Kainji, Nigeria

Seasonality and overlap of diet of Chrysichthys species in Kainji Lake was studied for a period of one year. A total of two hundred and forty five Chrysichthys nigrodigitatus and one hundred and nineteen Chrysichthys auratus longifilis were used during the study. Chrysichthys species food items ranged from plant to animal materials. Seasonal variations of diet showed that plant material dominated the diet of both species in rainy and dry seasons. While zygoptera and Lumbiculus were absent in the diet of Chrysichthys auratus longifilis in rainy season, zygoptera and nematode were absent in dry season. There was significant correlation (p< 0.01) of the items between the two species. There was also high level of association between their diets, which indicated that both species have similar diet. Lake Kainji is a good environment for the survival of Chrysichthys species. KEYWORDS: Chrysichthys species, overlap, diet, seasonality, Kainji Lake, Nigeria

Parasites of endemic and introduced fishes of Zaianderud River of the Esfahan

In this research, 9 species of local and introduced fishes of the Zayandehroud River in Esfahan province (in the Sarmatian region belonging to the large paleoarctic fauna) in 6 seasons (winter 2003, spring, summer, autumn and winter 2004 and summer 2005) were parasitologically studied. The local fishes included alburnoides bipunctatus, Alburnus maculatus, Aphanius vladykovi, Capoeta aculeata & Capoeta damascina & the introduced fishes included Aristichthys nobilis, Carassius auratus, Ctenopharyngodon idella and Cyprinus carpio. Upon being hunted, the fishes were transferred alive to Esfahan Aquatics Breeding Center and physiologically studied after the determination of their species and genus by identification keys Berg (30), Coad (31), Saadati (51), Abdoli (20) and Holchic (38). 32 species of parasites were totally identified as follows: 6 Protozoan species including Ichthyophthirius multifiliis, 5 Trichodina species, 2 Myxobolus species including Myxobolus cristatus & Myxobolus saidovi, 16 monogenea species including Dactylogyrus alatus. D. anchorutus, D. baueri, D. chalcalburni, D. chramuli, D. extensus, D. gracilis, D. lamellatus, D. lenkorani and D. pukher, 4 Dactylogyrus spp. 2 Gyrodactylus species, 1 species of Digenea, Diplostomum spthaceum, 4 species of Cestoda including Bothriocephallus gowkongensis, khawia armeniaca, Ligulaintestinalis. Caryophyllaeus sp. 1 Acanthocephala: Acanthocephalo rhynchoides cholodkowsky, 2 species of the crustaceans including the mature & copepodian stages of Lernaea cyprinacea & 1 sp of the genus Lamproglena. Out of all the 166 pcs of the fishes hunted in this research, 127 fishes (76.5%) were infected, and 39 fishes (23.50%) were not infected. In the fishes studied, having 14 of 32 species of the parasites identified, Capoeta aculeata displayed the most variety of infection, and having only 1 sp of the parasites. Aristichthys nobilis displayed the least variety of infection. The new findings of the research will follow: Myxobolus saidovi sp is reported for the 1st time from Iran's fresh water fishes, Alburnus maculatus and Capoeta aculeata are new hosts for M. saidovi and M. cristatus, respectively. Regarding monogenea Capoeta damascina & C. aculeata were reported as the new hosts for parasite D. pukher. The presence of D. pukher the infection of Capoeta aculeata with D. chramuli, D. lenkorani and D. gracilis in the Zayandehroud river were the 1st report. Regarding the Cestodea, Bothriocephalus gowkongensis was reported to be hosted by Aphanius Vladykovi for the 1St time in Iran.

Study of parasites native and introduced species of fish in Caspian Sea

Seven native and introduced species of fish in south east Caspian Sea coast examined for parasite infestation during 2004-2006. Native fishes include Barbus capito, Carassius auratus, Cyprinus carpio, Rutilus frisii kutum, Rutilus rutilus, Stizostidion lucioperca, Alosa caspia persica, 24 ecto and endo parasites were found in different organs of 7 species of fishes of them 2 of the metazoan 12 species of crustacean Lernaea cyprinacea , Lamproglena pukhella nematodea and cestodea parasite were found and identified to species and genus including: Asymphylodora kubanicums, Caryophylaeus fimbericep, Rhabdochona hellichii, Contracecum sp. (larvae), Pronoprymna, Aspidogaster limacoides, Raphidascaris acus, Caryophylaeus laticeps, Rhabdochona hellichi, Clinostomum complanatum, Hysterothylacium sp., Rhipidocotyle illense of the metzoan 9 monogen species were found and identified to species and genus level including Dactylogyrus frisii, Dactylogyrus nybelini, Dactylogyrus extensus, Gyrodactylus sp, Dactylogyrus baueri, D. formosus, Gyrodactylus sprostonae, Gyrodactylus sp, Mazaocraes alosae. One psecies of digenea parasites belonging to Diplostomatidea family comprised Diplostomum Spathaceum. In comprise infestation parasite of fish species Cyprinus carpio, Rutilus frisii kutum with 25 precent and Stizostidion lucioperca with 5 percent showed the highest and lowest in infection between fishes in comprise ecological region rivers with 45 percent and Estuary 16 percent showed the highest and lowest percent of parasite Infection.