Manual for starch gel electrophoresis: A method for the detection of genetic variation

The procedure to conduct horizontal starch gel electrophoresis on enzymes is described in detail. Areas covered are (I) collection and storage of specimens, (2) preparation of tissues, (3) preparation of a starch gel, (4) application of enzyme extracts to a gel, (5) setting up a gel for electrophoresis, (6) slicing a gel, and (7) staining a gel. Recipes are also included for 47 enzyme stains and 3 selected gel buffers. (PDF file contains 26 pages.)

Remote Camera and Trapping Survey of the Deep-water Shrimps Heterocarpus laevigatus and H. ensifer and the Geryonid Crab Chaceon granulatus in Palau

Time-lapse remote photo-sequences at 73-700 m depth off Palau, Western Caroline Islands, show that the caridean shrimp Heterocarpus laevigatus tends to be a solitary animal, occurring below ~350 m, that gradually accumulates around bait sites over a prolonged period. A smaller speies, H. ensifer, tends to move erratically in swarms, appearing in large numbers in the upper part of its range (<250 m) during the evening crepuscular period and disappearing at dawn. Trapping and photsequence data indicate the depth range of H. ensifer (during daylight) is ~250-550 M, while H. laevigatus ranges from 350 m to at least 800 m, along with the geryonid crab Chaceon granulatus. Combined trapping for Heterocarpus laevigatus and Chaceon granulatus, using a three-chamber box-trap and extended soak times (48-72 hr), may be an appropriate technique for small-scale deep-water fisheries along forereef slopes of Indo-Pacific archipelagoes.

On the Distribution and Fishery Potential of the Japanese Red Crab Chaceon granulatus in the Palauan Archipelago, Western Caroline Islands

A deep-water trapping survey in the Palauan archipelago, Western Caroline Islands, has revealed an abundance of the Japanese red crab, Chaceon granulatus. The recorded depth range (250-900 m) is similar to that of other geryonids, but the large numbers of females caught below 700 m is atypical. Mean yields in excess of 5 kg crabs plus 1 kg shrimp, Heterocarpus laevigatus, by-catch per trap-night were attainable at optimum depths. Chaceon granulatus is apparently a very large geryonid, with maximum weights of 2.02 kg and 1.51 kg recorded for male and female specimens, respectively. A range of body colors was observed: Orange-red shades appear to dominate the deeper waters (below 500 m) while yellow-tan colors are more abundant in the upper reaches. Preliminary evidence suggests that Chaceon granulatus is highly marketable, and the infrastructure in Palau is such that crabs could either be marketed fresh locally or airfreighted to Japan as a quick-frozen product. The high post-trapping survival rates observed indicate that maintaining crabs in live-holding tanks may be a feasible option. The large catches and quality of deep-water crabs taken suggests that the Palauan population of Chaceon granulatus may be able to support a small-scale fishery. It is not yet known whether this population is unusually large or whether these findings typify the deep forereef fauna of the region.

Technical report of frame and catch assessment surveys of Lakes Edward and George; and the Kazinga Channel conducted in January and July 2011- 2013

This study aimed at evaluating the production levels in terms of catch estimates of the artisanal fisheries of the Edward-George system in addition to providing information on the facilities and services at landing sites and the composition, magnitude and distribution of fishing effort to guide development and management of the fisheries resources of the Edward and George lakes and Kazinga channel. Specifically, the study was expected to come up with the following outputs:- a) Information on the number of fish landing sites on the basin lakes; b) Information on the facilities available at the fish landing sites to service the fisheries sector ; c) Information on the number of fishers; d) Information on the number and types of fishing crafts; e) Information on the modes of propulsion of the fishing crafts; f) Information on the number types and sizes of fishing gears including the number of illegal fishing gears in the fishery; and g) Recommendations on development and management of the fisheries of the Edward and George lakes and Kazinga channel. h) Beach values in terms of annual catches and annual revenue from the water bodies.

Fisheries studies on Lake George and Kazinga Channel: first quarter final report June/July 2001

FIRRI surveyed the fisheries of Lake George and Kazinga Channel between 20th June and 20th July 2001. This was the second survey FIRRI has conducted for the ILM project on the water system. The first survey was conducted during November 2000. These data, the analyses and accompanying reports contribute to baseline information for the fishery being collected with the support of ILM that is required for lakewide planning and management. Eight fish landing sites (6 on Lake George) namely; Kahendero, Hamukungu, Kasenyi, Kashaka, Mahyoro, Kayinja (2 on Kazinga Channel) namely; Katunguru -K and B fall within the focus of ILM and were surveyed during November 2000 and June/July 2001 over a three day period at each landing site in 2001 (Mahyoro 2 days). In November 2000, each landing was sampled once. FIRRI conducted a rapid FS and concurrently a CAS. All results are reported by landing site and then summed up (Global) for 8 sites on Lake George and Kazinga Channel.

The fisheries and fish stocks of Lake George: their productivity, exploitation, management and conservation

The study was confined to the fisheries of Lake George. The fishery of Lake George has been exploited under controlled exploitation but the permitted number of boats was fixed in the 1950s before the human population increased to the current level. Many more people were involved in fishing and it was feared that the fish stocks might not support the human population. The assignment involved preparation of a research proposal, collection of field data and production of a report in a period of eight months.