Growth variation and stock structure in North Pacific albacore

Working paper NPALB/87/21 submitted to the 10th North Pacific Albacore Workshop. Paper reports the results of ongoing research on validated age and growth models and the elucidation of stock structure for the North Pacific albacore. (Document pdf contains 22 pages)

Variation in size of yellowfin tuna (Thunnus albacares) within individual purse-seine sets

ENGLISH:Length-frequency samples of yellowfin tuna from 276 individual purse-seine sets were examined. Evidence of schooling by size is presented. Yellowfin schooled with skipjack are smaller and more homogeneous in length than are yellowfin from pure schools. Yellowfin in schools associated with porpoise appear to be more variable in size than yellowfin from other types of schools. No relationship was found between the tonnage of yellowfin in a school and the mean length of the yellowfin. Despite the tendency to school by size, the size variation within individual schools was judged to be enough to complicate greatly any program of regulation aimed at maximizing the yield-per-recruit through increasing the minimum size of yellowfin at first capture. SPANISH: Fueron examinadas las muestras frecuencia-longitud de atún aleta amarilla, de 276 lances individuales de redes de cerco. Se presenta la evidencia de agrupación por tamaños. Los atunes aleta amarilla agrupados con barrilete, son más pequeños y más homogéneos en longitud, que los atunes aleta amarilla de cardúmenes puros. El atún aleta amarilla en cardúmenes asociados con delfines parece ser más variable en tamaño, que el atún aleta amarilla proveniente de otros tipos de cardúmenes. No se encontró relac¡'ón entre el tonelaje del atún aleta amarilla en un cardumen y la longitud media de esta especie. A pesar de la tendencia a agruparse por tamaño, se juzgó, que la variación de tamaño en cardúmenes individuales, sería suficiente para complicar grandemente cualquier programa de reglamentación, dirigido a obtener el máximo del rendimiento por recluta a través del incremento del tamaño mínimo del atún aleta amarilla en la primera captura.

Manual for starch gel electrophoresis: A method for the detection of genetic variation

The procedure to conduct horizontal starch gel electrophoresis on enzymes is described in detail. Areas covered are (I) collection and storage of specimens, (2) preparation of tissues, (3) preparation of a starch gel, (4) application of enzyme extracts to a gel, (5) setting up a gel for electrophoresis, (6) slicing a gel, and (7) staining a gel. Recipes are also included for 47 enzyme stains and 3 selected gel buffers. (PDF file contains 26 pages.)

Meristic variation in Sebastes (Scorpaenidae), with an analysis of character association and bilateral pattern and their significance in species separation

This report presents meristic data for nearly all of the known species of Sebasles. Rudimentary caudal ray counts tend to be higher in more active species. The number of caudal rays supported by the hypurals is consistently 14, whereas the number of branched caudal rays varies between 11 and 13. Vertebral counts and most fin-ray counts tend to be lower in species or populations in warmer latitudes, except for pectoral ray counts which tend to have an opposite geographic pattern. On the basis of the small magnitude of meristic and morphometric differences and the lack of other differences between northern and southern samples of "Sebasles caurinus," Sebaslichlhys vexillaris Jordan and Gilbert is regarded as a junior synonym of Sebasles caurinus Richardson. The patterns of bilateral variation in paired meristics are analyzed and their mechanism discussed. The frequency distribution of pectoral ray counts in their right-left combination is shown to be useful in species separation. No association was found between any combination of two meristic features in any species. The author proposes that intrasample associations between meristic features are evidence of sampling heterogeneity. (PDF file contains 21 pages.)

An evaluation of geographic and annual variation in morphometric characters and gill-raker counts of yellowfin tuna, Thunnus albacares, from the Pacific Ocean

ENGLISH: Samples of yellowfin tuna, Thunnus albacares, collected from five areas of the Pacific Ocean (Mexico, Ecuador, Australia, Japan, and Hawaii) between January and May of 1988 and 1990 were examined for spatiotemporal variation in morphometric characters and gill-raker counts. 'Iwo-factor analysis of variance, with area and year treated as grouping factors, indicated a significant difference in the means of the total gill-raker counts among fish from different areas, but no significant difference between fish caught in different years. The morphometric data were adjusted by allometric formulae to remove size effects. The correct classification rates for the five groups, using discriminant function analysis, based on adjusted morphometric characters, were 77.60/0 for the samples from 1988 and 74.40/0 for those from 1990. These are 72.00/0 and 68.00/0 (Cohen's kappa statistic) better than would have occurred chance. The pattern of geographic variability, however, is unstable for these two years, thus requiring separate discriminant functions for each year. Although there is annual variability in the morphometric characters, these results demonstrate that the stocks examined are morphometrically distinguishable and that their phenetic relationships reflect their geographic origin. SPANISH: Se examinaron muestras de atún aleta amarilla, Thunnus albacares, tomadas de cinco áreas del Océano Pacífico (México, Ecuador, Australia, Japón, y Hawaii) entre enero y mayo de 1988 y 1990, para descubrir variaciones espaciotemporales en las características morfométricas y los conteos de branquiespinas. Un análisis de varianza de dos factores, con área y año como factores de agrupación, indicó una diferencia significativa en los promedios de los conteos de branquiespinas totales entre peces de distintas áreas, pero ninguna entre peces capturados en distintos años. Se ajustaron los datos morfométricos con fórmulas alométricas para eliminar los efectos de la talla del pez. En un análisis de función discriminante, las tasas de clasificación correcta de los cinco grupos, basadas en características morfométricas ajustadas, fueron 77.60/0 para las muestras de 1988 y 74.40/0 para aquellas de 1990. Estas cifras son 72.00/0 y 68.00/0 (estadístico de kappa de Cohen) mejores de lo que se hubiera obtenido al azar. Sin embargo, la variabilidad geográfica es inestable en estos dos años, requiriendo por lo tanto funciones discriminantes separadas para cada año. Aunque existe variabilidad anual en las características morfométricas, estos resultados demuestran que los stocks examinados son morfamétricamente distinguibles, y que su relación fenética refleja su origen geográfico. (PDF contains 31 pages.)

The variation of water quality in the outdoor concrete tank and earthen pond

The paper reports on the study carried out at the Federal College of Freshwater Fisheries and Technology (Nigeria) and the Hatchery Complex in 1997. The physico-chemical parameters of an earthen fish pond and concrete tank were analysed. The abundance and distribution of phytoplankton and zooplankton were recorded. A total of eight species of phytoplankton were observed. These species can be classified as Chlorophyceae (green algae), Bacillariophyceae (diatom algae) and Cyanophyta (blue-green algae). Eight zooplankton species were observed in the pond and 6 in the tank. Moina was observed and recorded as the most abundant cladoceran species in the tank

Seasonal variation of some trace metal concentrations in some fish species on stretch of River Kubanni, Zaria

In a semi-urbanized stretch of river Kubanni, Zaria, seasonal variations of some tree metals (copper, zinc and lead) in fish species (Clarias sp., Tilapia sp and Alestes sp) were investigated over a period of eight months. Water and fish were sampled monthly, pooled separately and seasonal analysis of each trace determined using atomic absorption method. The concentration of each of the elements in water higher in the dry season than during the rainy season. The dry and rainy season concentrations of copper, zinc and lead were 6.85~c10.66 mu gg super(-1)&1.45~c1.10 mu gg super(-1); 2.13~c1.68 mu gg super(-1)&0.1 5~c0.05 mu gg super(-1); and 0.52~c0.50 mu gg super(-1) & 0.31~c0.14 mu gg super(-1) respectively. Similarly, all the three accumulated more zinc and lead in the dry season than during the rainy season. Tilapia species and to some extent, Alestes species being pelagic had a higher concentration of trace metals than Clarias species which is a bottom dwelling fish Tilapia species may therefore be a better indicator species than Clarias species for monitoring trace metals in water bodies. Reasons for the observed variations in the trace metals were discussed

Variation der Gehalte an flüchtigen stickstoffhaltigen Basen und "TVB-N" in Rotbarsch

Die Gehalte an den flüchtigen stickstoffhaltigen Aminen, Amoniak, Dimethylamin (DMA), Trimethylamin (TMA) sowie Trimethylaminoxid (TMAO) und der durch Direktdestillation nach milder Alkalisierung mit Magnesiumoxid erhaltende Wert an sogenanntem flüchtigen Basenstickstoff (TVB-N) wurden an fangfrischen Exemplaren von Rotbarsch untersucht.

Morphological variation with interspecific implications in a population of Sigara dorsalis (Leach) (Hemiptera Heteroptera: Corixidae), and a request for British specimens

Sigara dorsalis belongs to a very closely related group of six species forming the sub-genus Sigara sensu strictu. Each of the six species has a distinct allopatric geographical distribution in Europe. Studies were started on a series of populations in the north west Midlands of England. All the populations examined, except one, contained only males with the typical diagnostic features of S. dorsalis, albeit with considerable variation. One pond near Congleton, Cheshire situated in a permanent-ley pasture and apparently free from pollution contained typical S. dorsalis males but, in addition, many atypical individuals. From one sample of forty-six males, all possessed left parameres with the slight point on the dorsal surface characteristic of S. dorsalis. However, almost half possessed additional morphological features intermediate between S. dorsalis and S. striata.

Seasonal variation in the diurnal periodicity of activity of the perch, Perca fluviatilis L. [Translation from: Kalamies 1973(3) 3.]

The most common catch of the amateur angler is the perch and it is the diurnal periodicity of activity (& catchability) which is examined in this study based on earlier articles and manuscripts by the authors. Of all environmental factors, variation in light and temperature are the chief reasons in establishing the times of activity periods. Winter, summer and autumn activity was studied. The spawning perch was found to be more active than the non-spawning perch. The time of day in which the fish may be active is dependant on its ability to sense changes in the external environment. Its adaptation to light is the reason for day-activity in the winter, and also accounts for the fact that hardly any activity occurs between sunset and sunrise when this period exceeds 6 hours.

Ecological significance of seasonal variation in clutch-size of Arctodiaptomus bacillifer in alpine lakes of high altitudes (Val Bognanco) [Translation from: Bollettino di Zoologia 37 491-492, 1970]

This brief paper describes the significance of seasonal variation in clutch-size of the copepod Arctodiaptomus bacillifer in alpine lakes of high altitudes (Val Bognanco). Seasonal dynamics of the zooplankton of these lakes was studied during summer and autumn of 1968 and 1969and results are summarised.

Variation in bacterial populations in time and space

Obtaining a reliable estimate of the bacterial population is one of the main problems facing the bacterial ecologist. The author discusses the various methods available and concludes that the observed variability in bacterial populations depends on the sampling interval used.

Description of sampling stations, methods of benthic sampling and biological water quality assessment; with some consideration of the influence of sample variation on the assessment values obtained

The first bilateral study of methods of biological sampling and biological methods of water quality assessment took place during June 1977 on selected sampling sites in the catchment of the River Trent (UK). The study was arranged in accordance with the protocol established by the joint working group responsible for the Anglo-Soviet Environmental Agreement. The main purpose of the bilateral study in Nottingham was for some of the methods of sampling and biological assessment used by UK biologists to be demonstrated to their Soviet counterparts and for the Soviet biologists to have the opportunity to test these methods at first hand in order to judge the potential of any of these methods for use within the Soviet Union. This paper is concerned with the nine river stations in the Trent catchment.

Corroborative studies on the effects of sample variation on three macroinvertebrate biological assessment methods at different water quality states

In recent collaborative biological sampling exercises organised by the Nottingham Regional Laboratory of the Severn-Trent Water Authority, the effect of handnet sampling variation on the quality and usefulness of the data obtained has been questioned, especially when this data is transcribed into one or more of the commonly used biological methods of water quality assessment. This study investigates if this effect is constant at sites with similar typography but differing water quality states when the sampling method is standardized and carried out by a single operator. An argument is made for the use of a lowest common denominator approach to give a more consistent result and obviate the effect of sampling variation on these biological assessment methods.

A Comparison of the SNP Variation in the Calcification PMCA Gene of Lottia gigantea between a Santa Barbara Population and a More Acidic Monterey Bay Population

As the atmospheric levels of CO2 rise from human activity, the carbonic acid levels of the ocean increase, causing ocean acidification. This increase in acidity breaks down the calcified bodies that many marine organisms depend upon. Upwelling regions such as Monterey Bay in California have pH levels that are not expected to reach the open ocean for a few decades. This study reviews one of the common intertidal animals of the California coast, the Owl Limpet Lottia gigantea, and its genetic variation of the plasma membrane Ca2+ ATPase (PMCA) in relation to the acidity of its environment. The PMCA protein functions in the calcification process of many organisms. Specifically in limpets, this gene functions to form its protective shell. Single-nucleotide polymorphisms (SNPs) were found among five sections of the gene to determine variation between the acidic environment population in Monterey, California and the non-acidic environment population in Santa Barbara, California. While some variation was determined, the Monterey Bay and Santa Barbara Lottia gigantea populations are not significantly distinct at the PMCA gene. Sections B, C, and D were found to be linked. Only one location in Section B was found to have an amino acid change within an exon. Section A has the strongest connection to the sampling location. Monterey individuals were seen to be more genetically recognizable, while Santa Barbara individuals showed slightly more variation. Understanding the trends of ocean acidification, upwelling region activities, and population genetics will assist in determining how the ocean environment will behave in the future.