First record of Holothuria (Platyperona) difficilis Semper, 1868 (Echinodermata:Holothuroidea) from the northern Arabian Sea (Pakistan)

During the 1994 winter collections, a small holothurian was collected from the rocky shore of Buleji (24°50'N, 66°53'E). It was attached to the green seaweed, Ulva fasciata and was not immediately noticed due to the same basic colour of the body as that of the seaweed. For identification a microscope slide of the spicules was prepared by placing a small piece of skin on a slide and treating it with (3.5%) sodium hypo-chloride (common household bleach). The specimen was identified as Holothuria (Platyperona) difficilis Semper, 1868.

A new record of Excirolana Orientalis (Dana, 1853), a cirolanid genus and species (Isopoda, Flabellifera) from the Pakistan coast

Excirolana orienta/is (Dana, 1853) belonging to the Cirolanidae, hitherto unknown from Pakistan, is collected from the rocky intertidal region of Manora Island, Karachi coast The specimens are fully described and illustrated. A list of the known species of the genus Excirolana is also provided since the genus is also first time reported from here.

Suggestive role of eye-stalk of Macrobrachium gangeticum Bate, 1868 in regulation of its reproductive behaviour

Role of eye-stalk of Macrobrachium gangeticum Bate, 1868 in its reproductive behaviour has been examined by conducting deletion and addition experiments. Eye-stalk ablation induced gonadal maturity in both sexes, leading to change in colour and size of ovaries and increase in GSI and oocyte diameter in females and increased length of testes and diameters of seminiferous tubules in males. Injection of eye-stalk extracts tended to at least partly restrict the effects in both sexes. The experiments thus suggested that the eye-stalk of M. gangeticum released some gonad inhibiting factors.