Sexual Reproduction and Early Development in the Estuarine Sea Anemone, Nematostella vectensis Stephenson, 1935

This dissertation: 1) determines the factor(s) responsible for spawning induction in NematosteJla vectensis; 2) isolates, describes, and documents the source of jelly from egg masses of N. vectensis; and 3) describes N. vectensis' early development. Namatostella vectensis were maintained on a 7-day mussel feeding/water change regime over 159 days. Within 36 hours of mussel feeding/water change. 69.1% of females and 78.5% of males spawned reliably. Through manipulation of feeding, water change, oxygen and nitrogenous waste concentrations, spawning induction was found to be triggered by the oxygen concentration associated with water change, and not by feeding. Ammonia, anemones' major waste product, inhibited this induction in a concentration-dependent manner. Female N. vectensis release eggs in a persistent jellied egg mass which is unique among the Actiniaria. The major component of this egg mass jelly was a positive periodic acid-Schiffs staining, 39.5-40.5 kD glycoprotein. Antibodies developed in rabbits against this glycoprotein bound to jelly of intact egg masses and to granules (~ 2.8 IJm in diameter) present in female anemone mesenteries and their associated filaments. Antibodies did not label male tissues. Nematostella vecfensis embryos underwent first karyokinesis -60 minutes following the addition of sperm to eggs. Second nuclear division took place, followed by first cleavage, 90-120 minutes later. Each of the 4 blastomeres that resulted from first cleavage contained a single nucleus. Arrangement of these blastomeres ranged from radial to pseudospiral. Embryonic development was both asynchronous and holoblastic. Following formation of the 4-cell stage, 71% of embryos proceeded to cleave again to form an 8-cell stage. In each of the remaining 29% of embryos, a fusion of from 2-4 blastomeres resulted in 4 possible patterns which had no affect on either cleavage interval timing or subsequent development. The fusion event was not due to ooplasmic segregation. Blastomeres isolated from 4-celled embryos were regulative and developed into normal planula larvae and juvenile anemones that were 1/4 the size of those that developed from intact 4-celled embryos. Embryos exhibiting the fusion phenomenon were examined at the fine structural level. The fusion phenomenon resulted in formation of a secondary syncytium and was not a mere compaction of blastomeres.

Reproduction, movements, and population dynamics of the southern kingfish, Menticirrhus americanus, in the northwestern Gulf of Mexico

Menlicirrhus americanus in the northwestern Gulf of Mexico mature at 150-220 mm TL and 12-14 months of age, with males maturing when 10-40 mm smaller than females. Spawning occurs within a broad period from February through November with two discrete peaks which coincide with the periodicity of downcoast alongshore currents (towards Mexico) in spring and fall. This species occurs at depths of less than 5 to 27 m, being most abundant at 5 m or shallower. Young-of-the-year recruit primarily at 5-9 m or shallower and gradually expand their bathymetric range. Age determination by length frequency is feasible in M. americanus but not as simple as in species that spawn in one major period of the year. Only one or two spawned groups normally predominated at anyone time and no more than three co-occurred with few possible exceptions. Observed mean sizes were 138 mm TL at 6 months, and 192 and 272 mm at ages I and II, respectively. Typical maximum size was 296-308 mm and typical maximum age is probably 2-3 years. The largest fISh captured were 392 and 455 mm. Observed sex ratio was 1.2 females to 1 male. Weight, girth, and length-length regressions are presented.(PDF file contains 27 pages.)

Reproduction, maturation, and seed production of cultured species: Proceedings of the Twelfth U.S.-Japan Meeting on Aquaculture, Baton Rouge, Louisiana, October 25-29, 1983

The United States and Japanese counterpart panels on aquaculture were formed in 1969 under the United States-Japan Cooperative Program in Natural Resources (UJNR). The panels currently include specialists drawn from the federal departments most concerned with aquaculture. Charged with exploring and developing bilateral cooperation, the panels have focused their efforts on exchanging information related to aquaculture which could be of benefit to both countries. The UJNR was begun during the Third Cabinet-Level Meeting of the Joint United States-Japan Committee on Trade and Economic Affairs in January 1964. In addition to aquaculture, current subjects in the program include desalination of seawater, toxic microorganisms, air pollution, energy, forage crops, national park management, mycoplasmosis, wind and seismic effects, protein resources, forestry, and several joint panels and committees in marine resources research, development, and utilization. Accomplishments include: Increased communication and cooperation among technical specialists; exchanges of information, data, and research findings; annual meetings of the panels, a policy-coordinative body; administrative staff meetings; exchanges of equipment, materials, and samples; several major technical conferences; and beneficial effects on international relations. (PDF file contains 79 pages.)

Preliminary observations on the feeding, habits and reproduction biology of Gymnarchus niloticus from Lake Chad

The preliminary results obtained from the study of stomach contents of specimens of Gymnarchus niloticus of a size ranging from 56 cm (66 grams) to 152 cm (12.0 kg.) from August to December, 1982 indicate for these sizes an exclusively piscivorous diet. The predominant prey species in Lake Chad are Tilapia/Sarotherodon , and Clarias . The prey is often cut in two parts before it is swallowed. Small prey may be swallowed whole. The implication of this prey capture method on prey-predator relationship and therefore the impact of the predator is discussed. The breeding season of Gymnarchus niloticus in Lake Chad has been deduced from observation of changes in gonad maturity stages and the results indicate that breeding takes place between August and November. Data are also presented on the fecundity, size of ripe eggs and probable size at maturity

Some aspects of the ecology and reproduction of the small pelagic cyprinid, Rastrineobola argentea in Lake Victoria

This study concentrated on the reproductive biology of the small pelagic cyprinid Rastrineobola argentea. The results indicate that this fish is an inshore spawning species, which agrees with other recent studies. It was also found that in areas where fishing intensity was likely to be relatively high, the size at first maturity of R. argentea was reduced, which is likely to be an effect of the fish altering its reproductive strategy according to life history theory. The CPUE results showed a general trend of decreasing with distance from shore, however areas less than one kilometer from the shore were not sampled. Evidence was also found suggesting that the cestode parasite, Ligula intestinalis had an adverse effect on the maturation and fecundity of R. argentea. Some management options concerning the findings in this study are also briefly discussed. (PDF has 82 pages)

The reproduction of Oreochromis niloticus (Linnaeus) in Opa reservoir, Ile-Ife, Nigeria

Oreochromis niloticus is one of the commercial fish species in Opa reservoir. It bred throughout the period of study in the reservoir. The fish species is a maternal mouth brooder with the female specimens carrying eggs and alevins in their mouths. The sex ratio of O. niloticus was 1:1 and the maximum fecundity recorded was 1810 eggs. The fecundity increased as the fish length increased. The relatively high fecundity of the fish species in the reservoir is an indication of the suitability of the small reservoir in contributing to fish production

Reproduction in Gammarus (Crustacea, Amphipoda): basic processes

Research on the basic reproduction processes of Gammarus is summarized and reviewed, reproductive strategies in males and females being left to two later papers. The author describes the reproductive systems, the development of eggs (oocytes) in the ovaries, courtship and precopulatory amplexus, mating and the production of sperms, egg laying, mortality and diapause.

Reproduction in Gammarus (Crustacea, Amphipoda): female strategies

This paper attempts to review the literature on Gammarus and examine how it allocates its internal resources when producing eggs. There is an extensive literature on the fecundity of freshwater species but almost nothing is known about the sizes and energy contents of the eggs. More is known for saltwater species, in which the mean number of eggs per brood is inversely proportional to mean egg size and directly proportional to the female's body size. Theoretical aspects of egg size, numbers and reproductive effort are examined, along with the relation between sizes of eggs, broods and female body size. The reproductive effort and breeding cycles of both saltwater and freshwater species are reviewed, and reproductive strategies assessed.

Growth and reproduction of Moina rectirostris (Leydig) and Ceriodaphnia quadrangula (O. F. Muller) with feeding on detritus. [Translation from: Sbornik po prudovomu rybovodstvu, 79-89. Moscow, VNIRO, 1969.]

The significance of detritus in the nutrition of aquatic animals, especially of the small representatives of the zooplankton, has been studied very slightly. Research so far has established that in M. rectirostris with feeding on protococcal and single-celled green algae and bacteria the beginning of formation of eggs takes place in 2 - 4 days. The young appear in 4 - 6 days. In this study M. rectirostris and C. quadrangula are fed on detritus of natural origin in laboratory conditions. In order to determine the assimilability and productive: effect of detritus, the author set up experiments, permitting to characterize the rate of growth, speed of maturing and fertility of M. rectirostris and C. quadrangula with feeding of them on detritus of different composition and age.

Reproduction de Ethmalosa fimbriata (Bowdich) en lagune Aby (Côte d'Ivoire)

Ethmalosa fimbriata (Bowdich) is the most abundant clupeid in the Ivorian artisanal fisheries. It represents over 60% of lagoon catches. In Aby lagoon the size at first sexual maturity is situated at 9.6 cm for male and 9.9 cm for female. The reproduction takes place twice a year during the high salinity periods from February to May and July to August. This reproduction does not affect significantly the sex-ratio, the mean of which varies from 35% to 36%, depending the period. The fecondity is very fluctuating and varies from 11000 eggs to 148000 eggs per female.

Reproduction et fécondité du machoiron Chrysichthys nigrodigitatus en élevage

Different rearing facilities (concrete tanks, pens and ponds) were tested for suitability as spawning environments. The concrete tanks and the pens in the lagoon gave the best results as to the number of spawns obtained. Of the three types of spawning devices tested, containers with a 150 mm opening at one of the two ends were preferably used by the fish. The brooders in the spawning facilities spontaneously entered the spawning containers to deposit their eggs without external human intervention. Actual fecundity estimates ranged from 9805 to 40597.

Biologie de l'Ethmalose, Ethmalosa fimbriata (Bowdich), en Côte d'Ivoire. 1 - Description de la reproduction et des premiers stades larvaires

Descriptions of spawning and larval development of Ethmalosa, up to the vitelline vesicle resorption stage, are made from plankton samplings in the Ebrié coastal lagoon and from artificially fertilized eggs. Spawning takes place from November to June in waters with salinities of 18 to 26 parts per thousand, and temperatures of 22.8 to 30.2 degrees, for 13-14 cm long fishes.

A note on the von Bertalanffy growth function concerning the allocation of surplus energy to reproduction

We propose an extended form of the von Bertalanffy growth function (VBGF), where the allocation of surplus energy to reproduction is considered. Any function can be used in our model to describe the ratio of energy allocation for reproduction to that for somatic growth. As an example, two models for energy allocation were derived: a step-function and a logistic function. The extended model can jointly describe growth in adult and juvenile stages. The change in growth rate between the two stages can be either gradual or steep; the latter gives a biphasic VBGF. The results of curve fitting indicated that a consideration of reproductive energy is meaningful for model extension. By controlling parameter values, our comprehensive model gives various growth curve shapes ranging from indeterminate to determinate growth. An increase in the number of parameters is unavoidable in practical applications of this new model. Additional information on reproduction will improve the reliability of model estimates.

Biomass and reproduction of Pacific sardine (Sardinops sagax) off the Pacific northwestern United States, 2003–2005

The Pacific sardine (Sardinops sagax) is distributed along the west coast of North America from Baja California to British Columbia. This article presents estimates of biomass, spawning biomass, and related biological parameters based on four trawl-ichthyoplankton surveys conducted during July 2003 –March 2005 off Oregon and Washington. The trawl-based biomass estimates, serving as relative abundance, were 198,600 t (coefficient of variation [CV] = 0.51) in July 2003, 20,100 t (0.8) in March 2004, 77,900 t (0.34) in July 2004, and 30,100 t (0.72) in March 2005 over an area close to 200,000 km2. The biomass estimates, high in July and low in March, are a strong indication of migration in and out of this area. Sardine spawn in July off the Pacific Northwest (PNW) coast and none of the sampled fish had spawned in March. The estimated spawning biomass for July 2003 and July 2004 was 39,184 t (0.57) and 84,120 t (0.93), respectively. The average active female sardine in the PNW spawned every 20–40 days compared to every 6–8 days off California. The spawning habitat was located in the southeastern area off the PNW coast, a shift from the northwest area off the PNW coast in the 1990s. Egg production in off the PNW for 2003–04 was lower than that off California and that in the 1990s. Because the biomass of Pacific sardine off the PNW appears to be supported heavily by migratory fish from California, the sustainability of the local PNW population relies on the stability of the population off California, and on local oceanographic conditions for local residence.

Age, growth, and reproduction of dolphinfish (Coryphaena hippurus) caught off the coast of North Carolina

Age, growth, and reproductive data were obtained from dolphinfish (Coryphaena hippurus, size range: 89 to 1451 mm fork length [FL]) collected between May 2002 and May 2004 off North Carolina. Annual increments from scales (n=541) and daily increments from sagittal otoliths (n=107) were examined; estimated von Bertalanffy parameters were L∞ (asymptotic length)=1299 mm FL and k (growth coefficient)=1.08/yr. Daily growth increments reduced much of the residual error in length-at-age estimates for age-0 dolphinfish; the estimated average growth rate was 3.78 mm/day during the first six months. Size at 50% maturity was slightly smaller for female (460 mm FL) than male (475 mm FL) dolphinfish. Based on monthly length-adjusted gonad weights, peak spawning occurs from April through July off North Carolina; back-calculated hatching dates from age-0 dolphinfish and prior reproductive studies on the east coast of Florida indicate that dolphinfish spawning occurs year round off the U.S. east coast and highest levels range from January through June. No major changes in length-at-age or size-at-maturity have occurred since the early 1960s, even after substantial increases in fishery landings.