Zum tierschutzgerechten Betäuben und Töten von Aal

Slaughtering of eels should be done not only under animal welfare aspects but as well under consumer protection and economical aspects with respect to technical feasibility. Methods must be practicable for enterprises slaughtering quantities of several kilograms up to several tons per day. Most methods applied up to now in whole Europe were (are) not in accordance with animal welfare mainly due to lacking prescriptions and alternative methods. The great number of experiments carried out within the frame of this project demonstrates the difficulties to combine optimal welfare demands with economical and technical fesibility aspects in one method. Measurements of EEG (electroencephalogram) and ECG (electrocardiogram) have shown that the method laid down in the german legislation does not perfectly stun all eels. This method was improved by applying a „prestun“ with 220 V for 1sec., followed by a 5 min phase at 45 V to prolongue the time of unconsciousness after the stun. Inflating nitrogen gas into the stunning bath additionally causes asphyxia during the stun. By this method 93% of the eels were stunned or even killed. Applying 220 V for bigger batches of eels (~400 kg) will cause big problems with the energy supply. Therefore this method is restricted to batches of 20 - 50 kg. The method laid down in the german legislation probably can be improved. Eels were stunned successfully without water, avoiding the problems of regional differences in the conductivity of the water. Other types of current and other frequencies and a combination of both should be tested as well as alternative electrode geometries also with respect to blood spots which occasionally occurred in the muscles preferably of bigger eels (>800 g). For the time being the method laid down in the german legislation is a reasonable compromise and under animal welfare aspects represents a considerable improvement.

White spot syndrome virus (WSSV) transmission risk through infected cooked shrimp products assessed by polymerase chain reaction (PCR) and bio-inoculation studies

The aim of the study was to evaluate the resistance of white spot syndrome virus (WSSV) in shrimps (Penaeus monodon) to the process of cooking. The cooking was carried out at 1000C six different durations 5, 10, 15, 20, 25 and 30 min. The presence of WSSV was tested by single step and nested polymerase chain reaction (PCR). In the single step PCR, the primers 1s5 & 1a16 and IK1 & IK2 were used. While in the nested PCR, primers IK1 &IK2 – IK3 & IK4 were used for the detection of WSSV. WSSV was detected in the single step PCR with the primers 1s5 and 1a16 and the nested PCR with the primers IK1 and IK2 – IK3 & IK4 from the cooked shrimp samples. The cooked shrimps, which gave positive results for WSSV by PCR, were further confirmed for the viability of WSSV by conducting the bio-inoculation studies. Mortality (100%) was observed within 123 h of intra-muscular post injection (P.I) into the live healthy WSSV-free shrimps (P. monodon). These results show that the WSSV survive the cooking process and even infected cooked shrimp products may pose a transmission risk for WSSV to the native shrimp farming systems.