8 resultados para Partial genomic libraries

em Aquatic Commons


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Power Point from Panel presentation giving implementation and search result displays and linking (17 slides)

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Niger River Basin Development Authority Minna (NRBDA) is one of the eleven river basins in Nigeria now undergoing transition towards partial commercialisation. In the light of this the need to be self-sustaining through exploration and exploitation of every possible areas along their operation to yield revenue cannot be over-emphasized. Therefore it is most pertinent to consider fisheries activities along their water bodies as one of the major sources of revenue by organising the local fishermen operating along the water into cooperative bodies and made to pay for fishing rights. Strategies to accomplish this objective is highlighted. The need to embark on aquaculture projects by construction of fish ponds at suitable sites along the reservoirs and developing the recreational potentials of their water bodies as sources of revenue is also stressed

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More and more libraries, museums and cultural institutions rely on fundraising and grant writing to sustain their services, special projects, or new initiatives Since most MLIS programs do not include grant project planning and proposal writing as part of their curriculum, librarians learn the process through trial and error, or continuing education classes. There are numerous books and websites that can assist the novice grant writer. We hope to provide beginners with basic information on the types of grants available, where to look for funding agencies, selected grant writing resources, and a few helpful grant writing hints to get you started. The presentation will be a three-person panel providing a short overview of the areas mentioned above, and discussion of sample grants submitted or received. The overview will be presented in Power Point format with an accompanying handout for each attendee.

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Procedures for sampling genomic DNA from live billfishes involve manual restraint and tissue excision that can be difficult to carry out and may produce stresses that affect fish survival. We examined the collection of surface mucous as a less invasive alternative method for sourcing genomic DNA by comparing it to autologous muscle tissue samples from Atlantic blue marlin (Makaira nigricans), white marlin (Tetrapturus albidus), sailfish (Istiophorus platypterus), and swordfish (Xiphias gladius). Purified DNA from mucous was comparable to muscle and was suitable for conventional polymerase chain reaction, random amplified polymorphic DNA analysis, and mitochondrial and nuclear locus sequencing. The nondestructive and less invasive characteristics of surface mucous collection may promote increased survival of released specimens and may be advantageous for other marine fish genetic studies, particularly those involving large live specimens destined for release.

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Ciguatoxins (CTX) are polyether neurotoxins that target voltage-gated sodium channels and are responsible for ciguatera, the most common fish-borne food poisoning in humans. This study characterizes the global transcriptional response of mouse liver to a symptomatic dose (0.26 ng/g) of the highly potent Pacific ciguatoxin-1 (P-CTX-1). At 1 h post-exposure 2.4% of features on a 44K whole genome array were differentially expressed (p ≤ 0.0001), increasing to 5.2% at 4 h and decreasing to 1.4% by 24 h post-CTX exposure. Data were filtered (|fold change| ≥ 1.5 and p ≤ 0.0001 in at least one time point) and a trend set of 1550 genes were used for further analysis. Early gene expression was likely influenced prominently by an acute 4°C decline in core body temperature by 1 h, which resolved by 8 h following exposure. An initial downregulation of 32 different solute carriers, many involved in sodium transport, was observed. Differential gene expression in pathways involving eicosanoid biosynthesis and cholesterol homeostasis was also noted. Cytochrome P450s (Cyps) were of particular interest due to their role in xenobiotic metabolism. Twenty-seven genes, mostly members of Cyp2 and Cyp4 families, showed significant changes in expression. Many Cyps underwent an initial downregulation at 1 h but were quickly and strongly upregulated at 4 and 24 h post-exposure. In addition to Cyps, increases in several glutathione S-transferases were observed, an indication that both phase I and phase II metabolic reactions are involved in the hepatic response to CTX in mice.

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This is a resume of a 1953-1955 study of Ceylon's fishing gears, fisheries and records of experimental and commercial fishing operations. Representative catches of edible fish per unit of effort for several of the gears studied are summarized in the table. They are low compared with many countries, indicating low abundance of fish.

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To find out a suitable balance diet for Cirrhinus. cirrhosus fry substituting fish meal protein by full-fat soybean meal protein as dietary protein source for C. cirrhosus fry (1.29g) was studied in 12 aquaria for 60 days. The fishes were fed with four iso-nitrogenous (30% protein) and iso-caloric experimental diets viz. diet 1 (100% fish meal protein), diet 2 (75% fishmeal protein and 25% soybean meal protein), diet 3 (50% fishmeal protein and 50% soybean meal protein) and diet 4 (25% fish meal protein and 75% soybean meal protein). Among the diets the best growth, FCR and PER were obtained with diet 3 fed fishes and no significant difference (p<0.05) was observed between diet 1 and 2 and diet 4 offered the worst performance. Therefore, considering fish growth, FCR and PER, it can be suggested that fish meal protein might be replaced 50% by soybean meal protein in the diet of C. cirrhosus fry for better growth.