Nachweis von Parvalbuminen in Störgeweben

Parvalbumins, calcium-binding, heat-stable proteins of considerable allergenic potential, are occurring in high concentration in light muscle of many fish species. Lack of knowledge about parvalbumins in sturgeon tissues prompted us to study the distribution of this type of proteins in light and dark muscle, as well as in swim bladder and skin of Atlantic sturgeon, Acipenser oxyrhynchus, and three other sturgeon species. Results: Light and dark muscle of sturgeons contained water soluble proteins with following properties: (1) Great stability against heating at 70 or 80 °C. (2) Isolelectric points between 3.85 and 5.66. (3) Treatment of proteins separated by isoelectric focusing with the dye “stains all” gave blue protein bands. (4) The molecular mass was about 10 to 14 kDalton. (5) Concentration of acidic, heat-stable proteins was higher in light than in dark muscle. Taken together, these findings gave strong indication for the presence of parvalbumins in muscle tissue of sturgeons. This conclusion was corroborated by immunological tests. However, parvalbumin could not be detected in swim bladder tissue. Skin preparations showed only traces of parvalbumins, possibly resulting from residual muscle tissue.

Fresh water fishery resources

The inland fresh waters of the island can be roughly divided into the following as far as fish production is concerned: (a) Perennial shallow irrigation reservoirs of the low-country, comprising about 120,000 acres. (b) " Villus" or flood lakes of the low country many of which are perennial, comprising about 30,000 acres. (c) Seasonal village tanks, mainly in the low-country, comprising about 30,000 acres. (d) Deep reservoirs (irrigation as well as hydro-electric) occurring in up-country and low-country comprising about 50,000 acres. (e) Rivers and streams comprising about 20,000 acres. The total area of all these waters is about 250,000 acres.

Experimente über die Wirkung von UV-B auf pelagische Fischembryonen in einem Sonnenscheinsimulator

One of the supposed effects of the observed ozone depletion is the increase of solar UV-B irradiation at the seasurface. This will cause an impact on certain compartments of marine ecosystems. Especially, sensitive developmental stages of pelagic fish embryos might be affected. Embryos of dab (Limanda limanda) and plaice (Pleuronectes plalessa) were experimentally exposed 10 different amounts of UVB irradiation in a sunshine simulator. This programmable device allows the dosage of realistic solar irradiation in quality and guantity. Experiments were carried out in March 1995 and February 1996. Either artificially inserninated and reared emhryos of dab and plaice or embryos caught in the German Bight were exposed to simulated solar irradiation. The 1995 experiments served to identify the effective irradiation dosages. For the 1996 experiments irradiation applied was much lower, being dose to realistic valucs expected over the North Sea as a consequence of ozone depletion. The following end points were studied: 1. Mortality, 2. sublethal morphological effects (malformations), 3. DNA damage, 4. changes in buoyancy of embryos measured as changes in osmolarity of the perivitelline fluid. Conditions for the simulation of daylight were a c1oudless sky with a solar zenith distance of 34 % (air mass 1.2). The adopted ozone depletion was 40 % corresponding to 180 DU (Dobson Units) instead of 300 DU. In the 1995 experiments time and dosage dependent influenccs on mortality and buoyancy of embryos of dab and plaice were found. Even in those embryos which were protected from the UV-B spectral range a loss of buoyancy was registered after 12 hours in the simulator. No diffcrences in DNA integrity as determined by DNA unwinding of exposed and control embryos were found. Also with lower amounts of irradiation in the 1996 experiments dosage dependent acute mortality, malformations, and impact on the buoyancy of the emhryos was registered. Sublethal effects occurred as well in embryos protected against UV-B in the exposure chambers, but were not found in the dark controls. The impact of low dosages of UV-B on the buoyancy of pelagic fish embryos might indicate an important ecological threat and deserves further studies.

Johann Heinrich von Thünen: Leben und Werk des Namenspatrons des neuen Bundesforschungsinstituts für Ländliche Räume, Wald und Fischerei

A new concept for resource oriented federal research within the competence of the German Ministry of Food, Agriculture and Consumer Protection was implemented in 2008. The Johann Heinrich von Thünen-Institute, Federal Research Institute for Rural Areas, Forestry and Fisheries is one of 4 newly established large scale federal research institutions. It comprises the former Federal Research Centre for Fisheries, the Federal Research Centre for Forestry and Forest Products and large parts of the Federal Agricultural Research Centre. The institute is named after Johann Heinrich von Thünen, a famous national economist and social reformer of the 19th century. He developed the first serious treatment of spatial economics (the so-called Thünen rings) and the basics of the theory of the natural pay.

Untersuchungen zur Sensibilität von Seefischen an Bord

First orientating investigations of the sensitiveness of the fish species dab (Limanda limanda), plaice (Pleuronectes platessa) and cod (Gadus morhua) immediately after catch and after different duration of bulk storage were carried out on board the fishing research vessel “Solea”. The aim of the investigations was to get an idea wether or not the central nervous system of the fishes was in function and the fishes could suffer pain. The proportion of sensitive dab, plaice and cod immediately after the catch was higher than 70, 80 and 95 %, respectively. After 30 min of bulk storage the percentage was reduced to 50, 60 and 70 % resp., after 90 minutes to 5, 15 % and 35 %. In cod it decreased further to 5 % after 120 min. An influence of trawling time (0,5 - 2 h) or size of the catch (100 to 1000 kg) on the number of sensitive animals was not found. Although fishing parameters in commercial fishery will in most cases put a stronger mechanical stress on the animals during trawling and handling on board a certain number of sensitive fish can still be expected.

Vergleich von Ausbeutefaktoren und Eislagerqualität zwischen Kliesche (Limanda limanda) und Scholle (Pleuronectes platessa) aus der Nordsee

North Sea plaice (Pleuronectes platessa) and dab (Limanda limanda) were experimentally stored in ice for 6 days during the 181th cruise of the FRV “Wather Herwig III”. It could be demonstrated that both flat fish species showed the same storage properties and were of a comparable quality until the end of the storage experiment. The quality of both species was determined by sensory assessment of the quality grade, by measuring of the impedance using the German Fischtester VI and the Icelandic RT- tester and pH- and TVB- N- measurements. The average length of North Sea dab is generally small (female: 18,5 ± 3,9 cm; male: 17 ± 2,9 cm), therefore it seemed to be more efficient to process fish portions (eviscerated, head, tail, fins and part of belly flaps removed). The yield by manually processed filets or fish portions from dab is about 30 or 62 %, respectively