Influence of salinity on population growth of a rotifer, Brachionus plicatilis (Mullen)

The rotifer Brachionus plicatilis plays an important role in prawn hatcheries. It tolerates a wide range of salinities. The present experiments were conducted to find the optimum salinity for its mass culture. Experiments conducted on various ranges of salinities starting from 0 to 40 ppt at an interval of 5 ppt revealed that Brachionus plicatilis did not survive at salinities 0 and 40 ppt. Optimum salinity studies conducted at 5-15 ppt with an interval of 1 ppt showed that the production of 70 individuals/ml was highest at 10 ppt salinity and the doubling time ranged from 1.728 to 1.317 days.

Breeding performance of Macrobrachium rosenbergii in captivity

The key factor in success of a Macrobrachium hatchery is to yield maximum number of larvae with minimum number of brooders. Suitable environmental conditions were provided and the breeding performance of females of M. rosenbergii was observed. A total number of 140 females brought from wild were used for this purpose. Each female was tagged and given a code number which made it possible to record the gestation period, interbreed period, number of breeding and larval yield of individual female during each breeding. Females were found to breed six times during the ten months duration. Of the total females studied, 99.12 % bred once, while only 2.16 % females bred six times in a total span of ten months. The average number of larvae decreased successively from first to fourth breeding. The average interbrood period was minimum between the first and second breeding and maximum between the fourth and fifth breeding. The highest average larval yield of 25,428 was recorded in the first breeding, the lowest of 4,596 in the fourth breeding. The possible reasons for variations in interbrood period and larval yield are discussed.

Effect of different feeds and water salinities on the cyst production of brine shrimp, Artemia sp.

Effect of different feeds and salinities on cyst production in Artemia sp. was studied. Among the 12 different feeds used, ragi powder suspension showed the best growth and cyst production. A wide range of salinity from 90 to 210‰ was used to study its effect on cyst production. Among all these treatments, 130‰ salinity was found suitable for induction of oviparity and cyst production in the San Francisco Bay strain of Artemia sp.

Effect of microwave pasteurisation on the quality of fish chikuwa

Temperature profile of fish chikuwa was taken during microwave cooking at 100 power level for different durations and subjected to organoleptic evaluation. Moisture content and organoleptic quality of fish chikuwa paste mixed with different levels of moisture and cooked at 100 power levels for 6 minutes were analysed. Microwave cooked fish chikuwa with standardized recipe was heated in microwave oven with hot air at different temperature for different durations. Fish chikuwa microwave cooked at 100 power level for 6 minutes had higher scores for all attributes as compared to those cooked for different durations and also fulfill the condition of pasteurisation of fish chikuwa. Fish chikuwa prepared with 35% moisture had better scores for all attributes unlike those of other levels. Heating of microwave pasteurised fish chikuwa at different temperatures for different durations could not achieve the desired brown colour.

Standardization and frozen storage of fish cutlet from bleached and unbleached mackerel mincemeat

In the present study, fish cutlets were prepared from bleached and unbleached mackerel mincemeat. Fish cutlets prepared from bleached meat had scored higher values for taste, flavour and overall acceptability as compared to those from unbleached mincemeat. Fish cutlets prepared with corn flour at the rate of 15% of fish mincemeat had scored higher values for all attributes as compared to other levels. Between the bleached and unbleached mincemeat, the scores for cutlet prepared with bleached mincemeat had higher score than that for the latter. There were no cracks in cutlets prepared with 15% and above corn flour levels as compared to those with lower levels. Fish cutlets prepared from bleached and unbleached mincemeat with spice mixture at 20 and 30% of the fish mince, respectively, had higher scores for taste, flavour, texture and overall acceptability as compared to those with other levels. Organoleptic quality of cutlet prepared from bleached and unbleached mackerel mince did not show changes in the appearance, colour and texture during storage. Changes were more prominent in flavour, taste and overall acceptability. Fish cutlets prepared from bleached mincemeat were acceptable for two months and those from unbleached mincemeat were acceptable up to one month from the point of view of organoleptic and biochemical qualities.

Preparation of pasteurized fish ball in curry and its storage study at 0 to 2°C

Fish ball in curry was prepared as per the standardized method and recipe, pasteurized and subjected to storage at 0 to 2°C in a domestic refrigerator. The curry prepared with tomato at a level of 20% of onion was found to be suitable organoleptically. A level of 3% of spice mixture improved the taste of curry. Fish balls prepared with concentrated curry paste and oil at a level of 0.5% was found to be suitable organoleptically. Curry prepared by adding oil at the rate of 3% scored high values for all attributes as compared to other levels. Curry prepared by diluting curry paste with water at rate of 20% had been adjudged the best as compared to other water levels. Fish ball in curry with a 50 : 50 ratio pasteurized at 85°C for five minutes had scored higher values under organoleptic evaluation. It was observed that the product was acceptable organoleptically up to the 12th day when stored at 0 to 2°C.

Standardization of polyphosphate treatment for tiny prawns (Parapenaeopsis stylifera) with special reference to its rehydration capacity and organoleptic quality

In the present study, an attempt was made to explore the benefits of polyphosphate for enhancement of dried prawn (Parapenaeopsis stylifera) quality commercially known as "sode" in Maharashtra coast. Dip treatment in polyphosphate solution at different concentrations (viz., 3, 5, 7 and 10%) was given to pealed and no deveined P. stylifera for different durations (viz., 1, 2, 3, 4 and 5 min). Treated prawns were dried and subjected to rehydration capacity test and organoleptic evaluation. Among the different treatments, rehydration capacity was found to increase with the increased duration and concentration of treatment. Tiny prawns treated with sodium tripolyphosphate solution at the rate of 5% concentration for 5 minutes showed an increase in pH from acidic to alkaline, and had better quality with respect to rehydration capacity and textural attributes as compared to other concentrations and durations of polyphosphate treatment.

Effect of modified starch on the shelf life of frozen fish Kamaboko with prepared spinach

Fish kamaboko with spinach was prepared by mixing fish kamaboko with spinach prepared with different combinations of ordinary starch (OS) and modified starch (MS), viz., 40:60, 50:50, 60:40, 100%) MS and 100% OS. The products were frozen at -40°C and stored at -20°C, and subjected to biochemical (peroxide value, total titratable acidity, pH and moisture) organoleptic (appearance, taste and colour), microbiological (total plate count) and physical (folding and expressible water) tests at monthly intervals. Among the different combinations tried, it was observed that fish kamaboko with spinach prepared with 50:50 and 60:40 combinations of OS and MS could be stored for six months at-20°C.

Standardisation of recipe for the preparation of lobster analogues

Lobster analogues were prepared with lobster base flavour (paste),h lobster cook water (whole lobster omogenate:water, 1:1 and 1:0.5) and lobster meat mince. In another experiment, different combinations of ginger-garlic paste and lobster base flavour, i.e., 1:3, 1:4, 1:6, 3:3, 3:4, 3:6, 5:3, 5:4 and 5:6 were added to the lobster analogue paste. It was observed that lobster analogues prepared with lobster base flavour (paste) are suitable organoleptically. The combination of ginger garlic paste and lobster base flavour in the ratio of 3:4 was found to be suitable organoleptically. Lobster analogues coloured with annatto seed colour at 1:2 (annatto seed:water) concentration had high values for the colour attribute as compared to orange-red synthetic colour, beetroot colour, caramel colour and paprika colour. It was observed that come-up-time to achieve a temperature of850°C was 28 minutes with a processing period of 11 minutes.

Shelf-life of horse mackerel fish balls stored at 0-2°C

The shelf-life of standardized horse mackerel fish balls was assessed by biochemical, microbiological, organoleptic and other spoilage changes at 0-2°C. There was decrease in pH value, moisture and the organoleptic scores. Expressible water percentage, TMA-N, TVB-N and peroxide value showed increasing trends. Total plate count also increased gradually during storage. Water separation in the treated sample was observed after 12 days and slimy consistency was noticed in the control sample on the 24th day. Based on these observations, it can be concluded that fish balls can be stored at 0-2°C for 20 days.

Rapapport's broth, a better enrichment medium in the identification of Salmonella from processed frog legs

Live clams (Villorita cyprinoides) collected from their natural beds were packed in different ways like dry pack, tray pack, in oxygenated water (wet pack) and depurated samples in wet pack. It was found that the packaging in l kg lots in 200 gauge polythene bags with oxygen at a temperature of 20°C could keep them live for 4 days. In tray pack without oxygen and water they can be kept alive for 3 days at 20°C. Temperature seems to be the critical factor in the transportation of live clams. At room temperature both dry and wet pack can be kept for 24 h only. Depuration technique does not appear to be useful in prolonging the storage life of clams in live condition as percentage mortality is more at 48 h both at 20°C and room temperature compared to the non-depurated samples.

Observations on the fish and prawn seed resources of the Gulf of Kutch and their utilization in aquaculture

The characteristics of the fish and prawn seed resources of the Gulf of Kutch are described. Results of experiments conducted in a primary low saline reservoir of a solar salt works to study their utility for aquaculture are reported. The prospective role of aquaculture in augmenting fish production along the coast is also discussed.