Mass culture of marine diatom Skeletonema costatum (Greville) Cleve collected from the Bay of Bengal

The growth of Skeletonema costatum in two artificial nutrient media was studied using various culture vessels. Skeletonema costatum was collected from the Cox's Bazar coast around the Bay of Bengal. Different growths stages i.e. lag phase, exponential phase, prestationary phase, stationary and death phase were observed during the culture period. The number of cells increased during the active division period and decreased after the beginning of the prestationary phase. The average densities of S. costatum in primary and secondary cultures were 0.55 x 10 super(6) cells mlˉ¹ and 0.93x10 super(6) cells mlˉ¹, respectively. In mass culture of S. costatum two, types of media were used. Highest cells densities of S. costatum cement tank culture were recorded 1.23x10 super(6) cell mlˉ¹ and 0.78x10 super(6) cells mlˉ¹ in their respective f/4 medium and commercial fertilizer medium. In the cement tanks culture fertilizer medium was found to be the best medium for mass culture of S. costatum in respect of production efficiency and culture stability.

Culture experiments on the copepod, Tisbintra elongata MORI, and evaluation of that species as food organism for milkfish larvae

Five types of food were used to culture T. elongata: rice bran, cow dung, bread, cow dung, bread yeast, fermented fish solubles and Spirulina. Bread yeast was found to give the highest densities. An evaluation was also made of the effectivity of rice bran and fermented fish solubles for the outdoor mass culture of T. elongata . A comparative study on the growth and survival rate of milkfish fry (Chanos chanos) fed with T. elongata and Artemia showed there to be no significant differences between the diets.

Monoculture of marine planktonic diatoms and the effects of different media on their growth and survival

Diatoms are the preferred live food of the protozoea stages of prawns. Due to seasonal variations, it is difficult to obtain continuous supply of diatoms and other algae throughout the year. Therefore mass culture of diatoms is necessary. At attempt was made to culture planktonic diatoms and to study the effect of Allen and Nelson media, Simon media, Vitamin B12 and treated sewage water media on their growth and survival. Navicula sp showed better growth in Allen and Nelson media, Coscinodiscus and Chaetoceros grew better in Simon media while Navicula sp and Coscinodiscus sp showed better growth in the combination of Allen and Nelson + Vitamin B12.

Influence of salinity on population growth of a rotifer, Brachionus plicatilis (Mullen)

The rotifer Brachionus plicatilis plays an important role in prawn hatcheries. It tolerates a wide range of salinities. The present experiments were conducted to find the optimum salinity for its mass culture. Experiments conducted on various ranges of salinities starting from 0 to 40 ppt at an interval of 5 ppt revealed that Brachionus plicatilis did not survive at salinities 0 and 40 ppt. Optimum salinity studies conducted at 5-15 ppt with an interval of 1 ppt showed that the production of 70 individuals/ml was highest at 10 ppt salinity and the doubling time ranged from 1.728 to 1.317 days.

The effect of spirulina (fresh and dry) on some biological factors in Penaeus semisulcatus larvae

Spirulina is a filamentous cyanobacteria with many applications in food and drug industries, as a food in human, aquaculture, vet and poultry industries… . Semi and mass culture of Spirulina carries out in different countries. This study was carried out in five phases in order to produce this microalga in Iran. The first phase, Spirulina pure stock was imported from Indonesia. After identification of species, it was cultured in laboratory until we took 20 liters of biomass. The semi-mass culture was carried out in green house. Cell concentration and size of Spirulina were recorded during culture daily and their growth rates were calculated. After two weeks, when the size of Spirulina was suitable, biomass of Spirulina was harvested then accumulated Spirulina weighted and dried in 24 hours in laboratory. In order to microbiological study, the samples of Spirulina (dry and fresh) were cultured on blood agar medium and coliforms were counted. The chemical composition of produced Spirulina was measured by standard methods. Fatty acid and amino acid profiles were acquired by GC and HPLC instruments, respectively. The amount of chlorophyll in Spirulina was determined by spectroscopy method. Also astaxanthin pigment as an important carotenoid was measured by HPLC in Spirulina and Penaeus semisulcatus larvae fed on Spirulina. At final phase of this project, larva fed on produced Spirulina (biomass and dry powder) was compared to Z plus, microencapsulated Spirulina (M.C.F) and Chaetoceros algae as control. This experiment was carried from zoa to early post larvae stage then survival and growth rate of larvae were recorded. The growth rate of larvae was evaluated with ANOVA test and survival rate of treatments was assessed by Log Rank (Mantel –Cox) test. Also during larvae stage, two parameters of water such as nitrate and nitrite were measured in zoa, mysis and post larvae stages. The results of this study were shown that colifom counts were 1.85×106 and 92.3×105 coliform per ml in fresh and dry spirulina, respectively. Protein percent of dry spirulina was 50.93 % (dry weight) and the amount of astaxanthin in spirulina and larvae fed on spirulina were 0.21 and 0.01 mg/kg, respectively. The most survival rate of larvae were observed in zoa III (88.8%) with Z plus supplement treatment, in mysis III (76.5%) combination of Z plus and dry spirulina in comparative between treatments. Larvae growth (4.5mm) of control in early post larvae was the best.

Some studies on Cladocera: Moina affinis Birge

Cladocera of Phyllopoda is very common and widely distributed in freshwater and there are more species of Cladocera than Copepoda. In Vietnam also, many kind of species of Cladocera were found by the authors. Since long time, Cladocera is known like one of the best feed for larval stage of fish. This report includes distribution and species of Cladocera in South Vietnam freshwaters; life history of Moina affinis; culture of Moina affinis and the mass culture experimented at the Oceanographic Institute of Nhatrang, Vietnam.

Shifting rice farming to fish culture in some selected areas of Mymensingh, Bangladesh: the process, conflicts and impacts

The evolutionary process of converting low-lying paddy fields into fish farms and its impact on agrarian communities in some selected areas of Mymensingh district were studied. This study was conducted through participatory rural appraisal (PRA) covering 12 villages from each of selected upazillas viz. Fulpur and Haluaghat of Mymensing [sic] district. A total of 12 PRA sessions were conducted where 90 farmers participated during 29 July to 26 August 2004. It is seen that the use of low-lying paddy fields was mostly confined to Broadcast Aman (B. Aman) rice production until 1960s. With the introduction of modern rice farming technology, the farmers started to produce Boro rice in Rabi season and B. Aman rice in Kharif season. With the passage of time, aquaculture technologies have been evolved and the farmers realized that fish farming is more profitable than rice cultivation, and then they started to utilize their paddy fields for alternate rice-fish farming and rice-cum-fish farming. Now a days, aquaculture based crop production system is in practice in more than 25% of the low-lying paddy fields. Conversion of rice fields in to fish ponds has brought up a change in the livelihood patterns of the rural farmers. The areas where the farmers involved themselves in the new production systems were fingerling collection, transportation and marketing of fry and fingerlings. During 1960s to 1970s, a few people used to culture fish in the permanent ponds for their own consumption, the species produced were rohu, catla, mrigal, ghainna, long whiskered catfish, freshwater shark (boal), snake head (shol) etc. Small fishes like climbing perch, stinging catfish, walking catfish, barb, minnows etc. were available in the rice fields during monsoon season. In 1980s to mid 1990s, some rice fields were converted into fish ponds and the people started to produce fish for commercial purposes. When rice-fish farming became profitable, a large number of people started converting their rice fields in to rice-fish culture ponds. Culture of some exotic fishes like silver carp, tilapia, grass carp, silver barb etc. also started in the paddy fields. Higher income from fish farming contributed positively in improving the housing, sanitation and education system in the study areas. It is seen that the medium and medium high lands were only used for alternate rice fish farming. The net income was high in any fish based cropping system that motivated the farmers to introduce fish based cropping system in the low-lying inland areas. As a result, the regional as well as communal income disparities occurred. However, the extraction of ground water became common during the dry period as the water was used for both rice and fish farming. Mass conversion of paddy fields into rice-fish culture ponds caused water logging in the study areas. In most cases, the participated farmers mentioned that they could be easily benefited by producing fish with T. Aman or only fish during the monsoon season. They agreed that this was an impressive technology to them and they could generate employment opportunities throughout the year. Finally, the social, economic and technical problems which are acting as constraints to rapid expansion of fish production system were reported from the interviewee.

Identification and culture of common diatoms as possible feed for Penaeus monodon

Diatoms were collected from Buyuan Bay, and from the hatchery tanks at Tigbauan, to determine the commonly occurring species, the feasibility of culturing these species, and the potential of these selected species as food for larval P. monodon. The commonly occurring diatoms were identified as Chaetoceros calcitrans, Navicula grimmei, Nitzchia seriata, Nitzchia closterium and Amphiprora sp. These diatoms were isolated and unialgal cultures prepared. Protein content analysis using the micro-Kjildahl method gave the following result: C. calcitrans, 11 . 78%; Nitzchia seriata, 25%; Nitzchia closterium, 30 . 5%; Navicula grimmei, 9 . 06% and Amphiprora sp. 8 . 96%. Feeding experiments were conducted to determine acceptability of the different diatom species and percentage survival of larval stages Z SUB-1 -M SUB-2 . Larvae were placed in 4-l capacity plastic containers with a stocking density of 10/l. The results of several feeding trials using the different mass-produced diatoms are summarized. From the data gathered, C. calcitrans appears to be the most promising candidate as feed for zoea and mysis stages of P. monodon. The average percentage survival of C. calcitrans was 63 . 76% for the 3 trials, and as high as 82 . 22% in the third trial. Comparatively high percentage survival of larvae was also recorded when Nitzchia seriata (48 . 17%) and Nitzchia closterium (67 . 6%) were given as feed, while both Amphiprora sp. and Navicula grimmei gave 0% survival. The poor results with Amphiprora sp. and Navicula grimmei may be due to their low protein content (8 . 96% and 9 . 06%, respectively) and the inability of the larvae to ingest them. Navicula and Amphiprora were observed to cling to the appendages of the larvae and to settle down in the medium making them unavailable to the larvae. Low survival was also noted when frozen C. calcitrans was used (14 . 25%). This may be due partly to the effect of the floculating agent (ALSO SUB-4 . 25 g/l) used in concentrating the diatoms. When protein contents of C. calcitrans, N. seriata and N. closterium are compared, the 2 Nitzchia species have relatively higher protein contents than C. calcitrans and, therefore, could be the more desirable feed candidates. However, few feeding trials were made using Nitzchia so that additional investigations will have to be done on this aspect.

Organic pollution in culture water resulting from excess feed and metabolite buildup

Penaeus monodon postlarvae were subjected to increasing feed concentrations and their growth and survival rates were recorded. Measurements were made of dissolved organic matter, and ammonia and nitrite-nitrogen concentrations. Survival was highest at the lowest feeding level and decreased as feed concentration increased. It is concluded that although organic matter enriches the food supply for P. monodon postlarvae, at higher concentration levels it can pollute the culture water, which in turn leads to mass mortality of the postlarvae. Secondly, the survival rate of P. monodon postlarvae is directly related to dissolved organic matter concentration, oxygen tension, and ammonia-nitrogen concentrations in the culture water. Even at sublethal levels these adverse environmental conditions decrease the survival rate.

Mass production of Penaeus monodon Fabricius juveniles in earthen nursery ponds

Different culture techniques were tried for rearing larvae of Penaeus monodon, in order to obtain preliminary data on stocking density, water management, fertilization versus feeding and effect of different types of vertical substrate. The results of the experiments showed that: (1) older fry have greater chances of survival; (2) the traditional nursery pond designs and practices used for milkfish in the Philippines are applicable to prawn only at very low densities and give acceptable high survival rates only when used with the older postlarval stage.