Activation mechanism of pre-phenoloxidase in lobster and shrimp

Phenoloxidase in shrimp and lobster is shown to exist in latent form which could be activated by trypsin and by an endogenous enzyme with tryptic activity. On Sephadex G-100 gel, three isoenzymes, differing in molecular weights, were isolated from naturally activated lobster shell extracts. Mechanism of activation of ptephenoloxidase involving limited proteolysis by the activating enzyme to form isoenzymes has been proposed.

Standardisation of recipe for the preparation of lobster analogues

Lobster analogues were prepared with lobster base flavour (paste),h lobster cook water (whole lobster omogenate:water, 1:1 and 1:0.5) and lobster meat mince. In another experiment, different combinations of ginger-garlic paste and lobster base flavour, i.e., 1:3, 1:4, 1:6, 3:3, 3:4, 3:6, 5:3, 5:4 and 5:6 were added to the lobster analogue paste. It was observed that lobster analogues prepared with lobster base flavour (paste) are suitable organoleptically. The combination of ginger garlic paste and lobster base flavour in the ratio of 3:4 was found to be suitable organoleptically. Lobster analogues coloured with annatto seed colour at 1:2 (annatto seed:water) concentration had high values for the colour attribute as compared to orange-red synthetic colour, beetroot colour, caramel colour and paprika colour. It was observed that come-up-time to achieve a temperature of850°C was 28 minutes with a processing period of 11 minutes.

Effects of the Maryland hydraulic clam dredge on populations of the soft-shell clam, Mya arenaria: final contract report

Final report on a three year study designed to investigate the effects of the Maryland hydraulic escalator clam dredge on populations and recruitment of the soft-shell clam, Mya arenaria. Experimental plots were established in the Potomac river, Maryland, and were dredged ina commerical manner by removing only legal size clams. quarterly samples were taken in the experimental and control plots by means of a van Veen grab for juvenile clams and the hydraulic dredge for older, deeper burrowing clams. Sediment samples were taken at selected periods for organic carbon and grain size analysis. Clams were separated into two size-groups. (PDF contains 38 pages)

The distribution and size composition of finfish, American lobster, and long-finned squid in Long Island Sound based on the Connecticut Fisheries Division Bottom Trawl Survey, 1984–1994

The distribution, abundance, and length composition of marine finfish, lobster, and squid in Long Island Sound were examined relative to season and physical features of the Sound, using Connecticut Department of Environmental Protection trawl survey data collected from 1984 to 1994. The following are presented: seasonal distribution maps for 59 species, abundance indices for 41 species, and length frequencies for 26 species. In addition, a broader view of habitat utilization in the Sound was examined by mapping aggregated catches (total catch per tow, demersal catch per tow, and pelagic catch per tow) and by comparing species richness and mean aggregate catch/tow by analysis of variance (ANOVA) among eight habitat types defined by depth interval and bottom type. For many individual species, seasonal migration patterns and preference for particular areas within Long Island Sound were evident. The aggregate distribution maps show that overall abundance was lower in the eastern Sound than the central and western portions. Demersal and pelagic temporal abundance show opposite trends—demersals were abundant in spring and declined through summer and fall, whereas pelagic abundance was low in spring and increased into fall. The analysis of habitat types revealed significant differences for both species richness and mean catch per tow. Generally, species richness was highest in habitats within the central area of the Sound and lowest in eastern habitats. The aggregate mean catch was highest in the western and central habitats, and declined eastward. (PDF file contains 199 pages.)

Catch-per-unit-effort and biological parameters from the Massachusetts coastal lobster (Homarus americanus) resource: Description and trends

Acomprehensive description of the Massachusetts coastal lobster (Homarus americanus) resou,rce was obtained by sampling commercial catches coastwide at sea and at dealerships between 1981 and 1986. Acommercial lobster sea-sampling program, wherein six coastal regions were sampled monthly, with an areal and temporal data weighting design, was the primary source of data. An improved index of catch per trap haul/set-over-day was generated by modeling the relationship between catch and immersion time and standardizing effort. This 6-year time-series of mean annual catch rates tracked closely the landings trend for territorial waters. During the study period there was a gradual increase in indices of exploitation and total annual mortality which corresponded to a gradual decline in mean carapace length of marketable lobster. The frequency of culls escalated from 10.0% in 1981 to 20.9% in 1986, while the percentage of lobster found dead in traps was consistently less than 1%. The sex ratio (%F:%M) was significantly different from 50:50 and approximated a 60:40 relationship during the study period. Male and female weight-length relationships were significantly different. Females weighed more than males at smaller sizes and less than males at larger sizes. A north-south clinal trend was evident wherein lobster north of Cape Cod weighed less at length than those from regions south of Cape Cod. Functional size-maturity relationships were developed for female lobster by staging cement gland development. Proportions mature at size represent more realistic values than those obtained by analyses of percent of females ovigerous. Regional variation occurred in most of the parameters studied. Three lobster groups, differing in major population descriptors, are defined by our data.(PDF file contains 28 pages.)

The Maryland soft shell clam industry and its effects on Tidewater resources

This report to the Maryland General Assembly covers: design and operation of the hydraulic clam dredge; summary of knowledge of Maryland's soft shell clam resource; development and present status; Potential value of the resource; Effects of the hydraulic clam dredge; evaluation of the effects of certain proposals concerning the soft shell clam industry; summary.

Management of fishing capacity in a spiny lobster (Panulirus argus) fishery: analysis of trap performance under the Florida spiny lobster Trap Certificate Program

The spiny lobster (Panulirus argus) fishery in Florida was operationally inefficient and overcapitalized throughout the 1980s. The Trap Certificate Program initiated during the 1992–93 season was intended to increase gear efficiency by reducing the number of traps being used while maintaining the same catch level in the fishery. A depletion model was used to estimate trap fishing efficiency. The costs of fishing operations and the value of the catch were used to determine the revenues generated by the fishery under different trap levels. A negative functional relationship was found between the catchability coefficient and the number of traps, which indicated that the fewer traps operating under the trap reduction scheme were more efficient. Also, the financial analyses indicated that the higher catch efficiency resulting from fewer traps generated significantly higher revenues, despite lower stock abundances. This study indicates that the trap reduction program had improved a situation that would have been much worse.

Mitochondrial DNA markers to identify commercial spiny lobster species (Panulirus spp.) from the Pacific coast of Mexico: an application on phyllosoma larvae

Molecular markers based on mitochondrial DNA (mtDNA) are extensively used to study genetic relationships. mtDNA has been used in phylogenetic studies to understand the evolutionary history of species because it is maternally inherited and is not subject to genetic recombination (Gyllensten et al., 1991). The high mutation rate of mtDNA makes it a useful tool for differentiating between closely related species (Brown et al., 1979)—a tool that is especially important when significant variations occur between species, but not within species (Hill et al., 2001; Blair et al., 2006; Chow et al., 2006a).

Changes in egg production of the western rock lobster (Panulirus cygnus) associated with appendage damage

Data collected during fish-ery-independent sampling programs were used to examine the impact of appendage damage (indicated by lost or regenerated legs and antennae) on the reproductive output of female western rock lobster (Panulirus cygnus). Most of the damaged females sampled had one (53%), two (27%), or three (13%) appendages that had been lost or that were regenerating. Appendage damage was associated with the reduced probability of a female developing ovigerous setae; and if setae were produced, with the reduced probability that females would produce more than one batch of eggs within a season. These effects were more pronounced as the number of damaged appendages increased. From data collected in 2002, it was estimated that the total number of eggs produced by mature females caught in the fishery was significantly reduced (P<0.001) by 3–9% when the impact of appendage damage was included.

A comparison of two fishery-independent survey programs used to define the population structure of American lobster (Homarus americanus) in the Gulf of Maine

The population structure and abundance of the American lobster (Homarus americanus) stock in the Gulf of Maine are defined by data derived from a fishery-independent trawl survey program conducted by the National Marine Fisheries Service (NMFS). Few sampling stations in the survey area are located inshore, in particular along coastal Maine. According to statistics, however, more than two thirds of the lobster landings come from inshore waters within three miles off the coast of Maine. In order to include an inshore survey program, complementary to the NMFS survey, the Maine Department of Marine Resources (DMR) initialized an inshore survey program in 2000. The survey was modeled on the NMFS survey program, making these two survey programs comparable. Using data from both survey programs, we evaluated the population structure of the American lobster in the Gulf of Maine. Our findings indicate that lobsters in the Gulf of Maine tend to have a size-dependent inshore-off-shore distribution; smaller lobsters are more likely to stay inshore and larger lobsters are more likely to stay offshore. The DMR inshore and NMFS survey programs focused on different areas in the Gulf of Maine and likely targeted different segments of the stock. We suggest that data from both survey programs be used to assess the lobster stock and to describe the dynamics of the stock in the Gulf of Maine.

Growth of spiny lobster (Panulirus penicillatus) caught off San Vicente, Cagayan, Philippines

Estimates of growth parameters for male and female Panulirus penicillatus caught in coral reef areas off San Vicente, Cagayan, Philippines are presented. Length-weight relationship parameters are also given. The results indicate that the slope (b) is significantly below 3.0 and does not differ significantly between males and females.

Use of a fishery independant index to predict recruitment and catches of the spiny lobster

This paper presents a review of recruitment and catch predictions based on an index of abundance of juveniles and pre-recruits (fishery independent index) in the Cuban lobster fisheries. This methodology can provide information based on fisheries data that can improve the management of the fishery.

The relationship of shell dimensions and shell volume to live weight and soft tissue weight in the mangrove clam, Polymesoda erosa (Solander, 1786) from northern Australia

Shell dimensions (length, height, width) and shell volume were evaluated as estimators of growth for Polymesoda erosa in northern Australia. Each parameter was a good estimator when applied to live weight (r2 values of 76-96 percent), but not to soft tissue weight (wet, dry, or ash-free dry weight) (r2 values of 13-32 percent). The b value for shell volume to weight relationship of clams collected during the dry season (June to October) was signifi cantly different than for those collected in the wet season (February to April).

On the growth, mortality and recruitment of the spiny lobster (Panulirus homarus) in Sri Lankan waters

Monthly length-frequency data of spiny lobster Panulirus homarus collected from the south coast of Sri Lanka during 1988-1990 were analyzed to estimate von Bertallanfy growth parameters. The asymptotic lengths estimated using Wetherall plots were 322 mm and 315 mm total length for the males and females, respectively. Using o' values of 3.53 for males and 3.61 for females, the growth constant (K) was estimated as 0.21 year super(1) and 0.27 year super(1) for the males and females, respectively. The estimates of natural and total mortality (M and Z) are 0.98 year super(1), 1.96 year super(1) for males and 0.92 year super(1), 1.54 year super(1) for females respectively. Recruitment appears to occur in two pulses per year.