Bowhead and white whale migration, distribution, and abundance in the Bering, Chukchi, and Beaufort Seas, 1975-78

From September 1975 to September 1977 we conducted field research on bowhead, Balaena mysticetus, and white, Delphinapterus leucas, whales in the U.S. Bering, Chukchi, and Beaufort Seas. The objectives were to determine the general distribution and migration of these whales in spring and autumn and to estimate abundance. We also surveyed the literature beginning in June 1975 through March 1978 to augment our empirical results. (PDF contains 48 pages)

Guide to the Identification of Larval and Early Juvenile Poachers (Scorpaeniformes: Agonidae) from the Northeastern Pacific Ocean and Bering Sea

Developmental stages of 22 species representing 16 genera of agonid fishes occurring in the northeastern Pacific Ocean from San Francisco Bay to the Arctic Ocean are presented. Three of these species also occur in the North Atlantic Ocean. Larval stages of nine species are described for the first time. Additional information or illustrations intended to augment original descriptions are provided for eight species. Information on five other species is provided from the literature for comparative purposes. The primary objective of this guide is to present taxonomic characters to help identify the early life history stages of agonid fishes in field collections. Meristic, morphometric, osteological, and pigmentation characters are used to identify agonid larvae. Meristic features include numbers of median-fin elements, pectoral-fin rays, dermal plates, and vertebrae. Eye diameter, body depth at the pectoral-fin origin, snout to first dorsal-fin length, and pectoral-fin length are the most useful morphological characters. Presence, absence, numbers, and/or patterns of dermal plates in lateral rows or on the ventral surface of the gut are also useful. Other important characters are the presence, absence, numbers, and ornamentation of larval head spines. Lastly, distinct pigmentation patterns are often diagnostic. The potential utility of larval characters in phylogenetic analysis of the family Agonidae is discussed. (PDF file contains 92 pages.)

Pictorial Guide to the Groupers (Teleostei: Serranidae) of the Western North Atlantic

This guide was developed to assist with the identification of western North Atlantic grouper species of the genera Alphestes, Cephalopholis, Dermatolepis, Epinephelus, Gonioplectrus, Mycteroperca, and Paranthias. The primary purpose for assembling the guide is for use with projects that deploy underwater video camera systems. The most vital source of information used to develop the guide was an archive of underwater video footage recorded during fishery projects. These video tapes contain 348 hours of survey activity and are maintained at the National Marine Fisheries Service (NMFS), Pascagoula, Mississippi. This footage spans several years (1980-92) and was recorded under a wide variety of conditions depicting diverse habitats from areas of the western North Atlantic Ocean, Caribbean Sea, and Gulf of Mexico. Published references were used as sources of information for those species not recorded on video footage during NMFS projects. These references were also used to augment information collected from video footage to provide broader and more complete descriptions. The pictorial guide presents information for all 25 grouper species reported to occur in the western North Atlantic. Species accounts provide descriptive text and illustrations depicting documented phases for the various groupers. In addition, species separation sheets based on important identification features were constructed to further assist with species identification. A meristic table provides information for specimens captured in conjunction with videoassisted fishery surveys. A computerized version enables guide users to amend, revise, update, or customize the guide as new observations and information become available. (PDF file contains 52 pages.)

Herpetofaunal Inventories of the National Parks of South Florida and the Caribbean: Volume II. Virgin Islands National Park

Amphibian declines and extinctions have been documented around the world, often in protected natural areas. Concern for this alarming trend has focused attention on the need to document all species of amphibians that occur within U.S. National Parks and to search for any signs that amphibians may be declining. This study, an inventory of amphibian species in Virgin Islands National Park, was conducted from 2001 to 2003. The goals of the project were to create a georeferenced inventory of amphibian species, use new analytical techniques to estimate proportion of sites occupied by each species, look for any signs of amphibian decline (missing species, disease, die-offs, etc.), and to establish a protocol that could be used for future monitoring efforts. Several sampling methods were used to accomplish these goals. Visual encounter surveys and anuran vocalization surveys were conducted in all habitats throughout the park to estimate the proportion of sites or proportion of area occupied (PAO) by amphibian species in each habitat. Line transect methods were used to estimate density of some amphibian species and double observer analysis was used to refine counts based on detection probabilities. Opportunistic collections were used to augment the visual encounter methods for rare species. Data were collected during four sampling periods and every major trail system throughout the park was surveyed. All of the amphibian species believed to occur on St. John were detected during these surveys. One species not previously reported, the Cuban treefrog (Osteopilus septentrionalis), was also added to the species list. That species and two others (Eleutherodactylus coqui and Eleutherodactylus lentus) bring the total number of introduced amphibians on St. John to three. We detected most of the reptile species thought to occur on St. John, but our methods were less suitable for reptiles compared to amphibians. No amphibian species appear to be in decline at this time. We found no evidence of disease or of malformations. Our surveys provide a snapshot picture of the status of the amphibian species, so continued monitoring would be necessary to determine long-term trends, but several potential threats to amphibians were identified. Invasive species, especially the Cuban treefrog, have the potential to decrease populations of native amphibians. Introduced mammalian predators are also a potential threat, especially to the reptiles of St. John, and mammalian grazers might have indirect effects on amphibians and reptiles through habitat modification. Finally, loss of habitat to development outside the park boundary could harm some important populations of amphibians and reptiles on the island.

Herpetofaunal Inventories of the National Parks of South Florida and the Caribbean: Volume III. Big Cypress National Preserve

Amphibian declines and extinctions have been documented around the world, often in protected natural areas. Concern for this trend has prompted the U.S. Geological Survey and the National Park Service to document all species of amphibians that occur within U.S. National Parks and to search for any signs that amphibians may be declining. This study, an inventory of amphibian species in Big Cypress National Preserve, was conducted from 2002 to 2003. The goals of the project were to create a georeferenced inventory of amphibian species, use new analytical techniques to estimate proportion of sites occupied by each species, look for any signs of amphibian decline (missing species, disease, die-offs, and so forth.), and to establish a protocol that could be used for future monitoring efforts. Several sampling methods were used to accomplish these goals. Visual encounter surveys and anuran vocalization surveys were conducted in all habitats throughout the park to estimate the proportion of sites or proportion of area occupied (PAO) by each amphibian species in each habitat. Opportunistic collections, as well as limited drift fence data, were used to augment the visual encounter methods for highly aquatic or cryptic species. A total of 545 visits to 104 sites were conducted for standard sampling alone, and 2,358 individual amphibians and 374 reptiles were encountered. Data analysis was conducted in program PRESENCE to provide PAO estimates for each of the anuran species. All of the amphibian species historically found in Big Cypress National Preserve were detected during this project. At least one individual of each of the four salamander species was captured during sampling. Each of the anuran species in the preserve was adequately sampled using standard herpetological sampling methods, and PAO estimates were produced for each species of anuran by habitat. This information serves as an indicator of habitat associations of the species and relative abundance of sites occupied, but it will also be useful as a comparative baseline for future monitoring efforts. In addition to sampling for amphibians, all encounters with reptiles were documented. The sampling methods used for detecting amphibians are also appropriate for many reptile species. These reptile locations are included in this report, but the number of reptile observations was not sufficient to estimate PAO for reptile species. We encountered 35 of the 46 species of reptiles believed to be present in Big Cypress National Preserve during this study, and evidence exists of the presence of four other reptile species in the Preserve. This study found no evidence of amphibian decline in Big Cypress National Preserve. Although no evidence of decline was observed, several threats to amphibians were identified. Introduced species, especially the Cuban treefrog (Osteopilus septentrionalis), are predators and competitors with several native frog species. The recreational use of off-road vehicles has the potential to affect some amphibian populations, and a study on those potential impacts is currently underway. Also, interference by humans with the natural hydrologic cycle of south Florida has the potential to alter the amphibian community. Continued monitoring of the amphibian species in Big Cypress National Preserve is recommended. The methods used in this study were adequate to produce reliable estimates of the proportion of sites occupied by most anuran species, and are a cost-effective means of determining the status of their populations.

Guide to the identification of larval and early juvenile pricklebacks (Perciformes: Zoarcoidei: Stichaeidae) in the northeastern Pacific Ocean and Bering Sea

Stichaeidae, commonly referred to as pricklebacks, are intertidal and subtidal fishes primarily of the North Pacific Ocean. Broad distribution in relatively inaccessible and undersampled habitats has contributed to a general lack of information about this family. In this study, descriptions of early life history stages are presented for 25 species representing 18 genera of stichaeid fishes from the northeastern Pacific Ocean, Bering Sea, and Arctic Ocean Basin. Six of these species also occur in the North Atlantic Ocean. Larval stages of 16 species are described for the first time. Additional information or illustrations intended to augment previous descriptions are provided for nine species. For most taxa, we present adult and larval distributions, descriptions of morphometric, meristic, and pigmentation characters, and species comparisons, and we provide illustrations for preflexion through postflexion or transformation stages. New counts of meristic features are reported for several species.

A preliminary description of climatology in the western United States

We describe the climatology of the western United States as seen from two 1-month perspectives, January and July 1988, of the National Meteorological Center large-scale global analysis, the Colorado State University Regional Atmospheric Modeling System (RAMS), and various station observation sets. An advantage of the NMC analysis and the RAMS is that they provide a continuous field interpolation of the meteorological variables. It is more difficult to describe spatial meteorological fields from the available sparse station networks. We assess accuracy of the NMC analysis and RAMS by finding differences between the analysis, the model, and station values at the stations. From these comparisons, we find that RAMS has much more well-developed mesoscale circulation, especially in the surface wind field. However, RAMS climatological and transient fields do not appear to be substantially closer than the larger-scale analysis to the station observations. The RAMS model does provide other meteorological variables, such as precipitation, which are not readily available from the archives of the global analysis. Thus, RAMS could, at the least, be a tool to augment the NMC large-scale analyses.

Translocation as a strategy to rehabilitate the queen conch (Strombus gigas) population in the Florida Keys

Queen conch (Strombus gigas) stocks in the Florida Keys once supported commercial and recreational fisheries, but overharvesting has decimated this once abundant snail. Despite a ban on harvesting this species since 1985, the local conch population has not recovered. In addition, previous work has reported that conch located in nearshore Keys waters are incapable of spawning because of poor gonadal condition, although reproduction does occur offshore. Queen conch in other areas undergo ontogenetic migrations from shallow, nearshore sites to offshore habitats, but conch in the Florida Keys are prevented from doing so by Hawk Channel. The present study was initiated to determine the potential of translocating nonspawning nearshore conch to offshore sites in order to augment the spawning stock. We translocated adult conch from two nearshore sites to two offshore sites. Histological examinations at the initiation of this study confirmed that nearshore conch were incapable of reproduction, whereas offshore conch had normal gonads and thus were able to reproduce. The gonads of nearshore females were in worse condition than those of nearshore males. However, the gonadal condition of the translocated nearshore conch improved, and these animals began spawning after three months offshore. This finding suggests that some component of the nearshore environment (e.g., pollutants, temperature extremes, poor food or habitat quality) disrupts reproduction in conch, but that removal of nearshore animals to suitable offshore habitat can restore reproductive viability. These results indicate that translocations are preferable to releasing hatchery-reared juveniles because they are more cost-effective, result in a more rapid increase in reproductive output, and maintain the genetic integrity of the wild stock. Therefore, translocating nearshore conch to offshore spawning aggregations may be the key to expediting the recovery of queen conch stocks in the Florida Keys.

A numerical simulation of cool/wet and warm/wet episodes in the western United States

Wintertime precipitation in the mountains of the western United States during a warm or cool period has a pronounced influence on streamflow. During a warm year, streamflow at intermediate elevations responds more immediately to precipitation events; during a cold year, much of the discharge is delayed until the snow melts in spring and summer. Previous efforts at studying these extremes have been hampered by a limited number and length of observational analyses. In this study, we augment this limited observational record by analyzing a simplified general circulation model.

Reproductive engineering in crustacean aquaculture

Crustacean aquaculture industry in India suffers greatly from lack of technological developments. A major constraint in this enterprise is the limitation of seed stock availability. A critical appraisal is made of the techniques used in the manipulation of reproductive processes in order to augment year-round production of seeds. A new possibility of induced ovarian maturation in crustaceans is by administering steroid hormones of vertebrate source. Environmental factors are known to govern the gametogenic cycle of marine crustaceans. Cryopreservation of male gametes and artificial insemination by way of spermatophore transfer could solve some of the problems of mating under laboratory conditions.

Effects of control of C/N ratio by low-cost carbohydrate addition on water quality and pond ecology in freshwater prawn Macrobrachium rosenbergii post-larvae nursing system

An experiment was conducted to evaluate the effects of control of carbon/nitrogen ratio (C/N ratio) by addition of low cost carbohydrate to the water column on water quality and pond ecology in freshwater prawn Macrobrachium rosenbergii post-larvae nursing system. In this experiment, two level of dietary protein 20% and 35% without carbohydrate addition (‘P20' and ‘P35') and with carbohydrate addition (‘P20+CH' and ‘P35+CH') were compared in small ponds of 40 m² area stocked with 20 post-larvae (0.021 ± 0.001g) per m² . Maize flour was used as low cost carbohydrate and applied to the water column followed by the first feeding during the day. The addition of carbohydrate significantly reduced (p< 0.05) ammonia-nitrogen (NH sub(3)-N) and nitrite-nitrogen (NO sub(2) - N) of water in P20 + CH and P35 + CH treatments. It significantly increased (p< 0.05) the total heterotrophic bacteria (THB) population both in water and sediment. Fifty nine genera of plankton were identified belonging to the Bacillariophyceae (11), Chlorophyceae (21), Cyanophyceae (7), Dinophyceae (1), Rotifera (7) and Crustacea (9) without any significant difference (p>0.05) of total phytoplankton and zooplankton among the treatments. Survival rate of prawn was significantly lowest (p<0.05) in P20 and no significant difference (p>0.05) was observed between P20+CH and P35 treatments. Control of C/N ratio by the addition of low-cost carbohydrate to the pond water column benefited the freshwater prawn nursing practices in three ways (1) increased heterotrophic bacterial growth supplying bacterial protein augment the prawn post-larvae growth performances, (2) reduced demand for supplemental feed protein and subsequent reduction in feed cost and (3) reduced toxic NH sub(3)-N and NO sub(2)-N levels in pond nursing system.

Introducing tilapia (GIFT) with shrimp (Penaues monodon) in brackishwater rice-shrimp system: impact on water quality and production

Mixed rearing of tilapia (Genetically Improved Farmed Tilapia, GIFT) with shrimp (Penaeus monodon) in brackishwater rice-shrimp system was assessed for its impact on dry season's shrimp production. The experiment was conducted in pre-selected farmer's field located at Paikgacha Upazila of Khulna district and designed with three different densities (treatment) of GIFT, viz, 0.3, 0.4 and 0.5/m² with a constant stocking density of shrimp at 3/m². Each treatment had three replications. There had a set of control treatment where GIFT was not stocked. Results of the experiment revealed that tilapia did not exert any significant effect (p>0.05) on the water quality variables, even on survival rate of shrimp (p>0.05) under farm level condition in rice-shrimp rotational system, but a density dependent negative effect (P<0.05) on the growth of shrimp led apparently lower production rate of shrimp. Though tilapia provided the major augment of total production (p<0.05) in the respective treatments than in monoculture of shrimp, but not that of the economic return. However, economic loss due to sudden shrimp crop failure might be partially minimized by the tilapia crop.