Paciano II: the report of a conversation on whaling and science, 1-3 March 2008 (Paciano, Umbria)

In 2006 the UK-based charity, Global Ocean, with local support from the Third Millennium Foundation, convened a Conversation among specialists about the problems facing the conservation of whales. Called "A consultation on whaling", this gathering was held in the ancient village of Paciano, in Umbria near the border with Tuscany, 15 – 17 October 2006. There were 15 participants from 11 countries. Dr Kees Lankester served as moderator. The outcome was an Aide Memoire which served to guide the participants in the run-up to the 2007 meeting of the International Whaling Commission (IWC), held in Anchorage, Alaska, in June. One point of agreement was that a second consultation should be held in the months following the Anchorage meeting, involving some but not necessarily all of the participants in the first, but concentrating this time on scientific issues – especially those encountered in the Scientific Committee of the IWC –with particular attention to informing a wider public about those scientific activities in relation to the problems confronting the IWC and the views of scientists about them. This document is the report of that Conversation, referred to as Paciano II. The moderator was Dr Giuseppe Notarbartolo di Sciara and the Report was written by Kieran Mulvaney in consultation with all participants, and with reference to an Aide Memoire prepared by the Rapporteur, Dr Russell Leaper. The sponsor and organisers have agreed with the general sentiment expressed by participants in Paciano II that further such conversations should be held at roughly yearly intervals and they will try to satisfy that desire. Although these future gatherings would be concerned with the living ocean they would not necessarily be restricted in future to consideration of whales and whaling. Discussions are on-going for selection of a theme which is of both scientific interest and practical concern for conservation of marine life and management of the uses of ocean space. (Document has 18 pages)

Paciano II: a report of a conversation on whaling and science, 1-3 March 2008

In 2006 the UK-based charity, Global Ocean, with local support from the Third Millennium Foundation, convened a Conversation among specialists about the problems facing the conservation of whales. Called "A consultation on whaling", this gathering was held in the ancient village of Paciano, in Umbria near the border with Tuscany, 15 – 17 October 2006. There were 15 participants from 11 countries. Dr Kees Lankester served as moderator. The outcome was an Aide Memoire which served to guide the participants in the run-up to the 2007 meeting of the International Whaling Commission (IWC), held in Anchorage, Alaska, in June. One point of agreement was that a second consultation should be held in the months following the Anchorage meeting, involving some but not necessarily all of the participants in the first, but concentrating this time on scientific issues – especially those encountered in the Scientific Committee of the IWC –with particular attention to informing a wider public about those scientific activities in relation to the problems confronting the IWC and the views of scientists about them. This document is the report of that Conversation, referred to as Paciano II. The moderator was Dr Giuseppe Notarbartolo di Sciara and the Report was written by Kieran Mulvaney in consultation with all participants, and with reference to an Aide Memoire prepared by the Rapporteur, Dr Russell Leaper. The sponsor and organisers have agreed with the general sentiment expressed by participants in Paciano II that further such conversations should be held at roughly yearly intervals and they will try to satisfy that desire. Although these future gatherings would be concerned with the living ocean they would not necessarily be restricted in future to consideration of whales and whaling. Discussions are on-going for selection of a theme which is of both scientific interest and practical concern for conservation of marine life and management of the uses of ocean space. (19 page document)

PICES Press, Vol. 15, No. 1, January 2007

PICES science – 2006 (pdf, 0.1 Mb) 2006 Wooster Award (pdf, 0.1 Mb) Thank you note from the Past-Chairman of PICES (pdf, 0.1 Mb) A comparison of regional mechanisms for fish production: Ecosystem perspectives (pdf, 0.3 Mb) 2006 CREAMS/PICES international workshop and summer school (pdf, 0.2 Mb) PICES Calendar (pdf, 0.2 Mb) 2006 Harmful Algal Bloom Section annual workshop (pdf, 0.1 Mb) 2006 PICES Workshop on “Modeling iron biogeochemistry and ocean ecosystems” (pdf, 0.1 Mb) Strolling through the NEMURO ecosystem model (pdf, 0.1 Mb) Climate and marine birds and mammals in the North Pacific (pdf, 0.2 Mb) Photo highlights of the PICES Fifteenth Annual Meeting (pdf, 3.5 Mb) Recent trends in waters of the subarctic NE Pacific: Cooler and fresher in summer of 2006 (pdf, 0.2 Mb) The state of the western North Pacific in the first half of 2006 (pdf, 0.3 Mb) Latest and upcoming PICES publications (pdf, 0.3 Mb) A seven-year effort of the PICES CCCC MODEL Task Team culminates in a dedicated issue of Ecological Modelling (pdf, 0.1 Mb) Japan joins PICES Marine Metadata Federation (pdf, 0.3 Mb) Argo: A 2006 status report (pdf, 0.3 Mb) New Chairmen in PICES (pdf, 0.2 Mb) PICES Interns (pdf, 0.2 Mb)

PICES Press, Vol. 14, No. 1, January 2006

The state of PICES science - 2005 (pdf, 0.2 Mb) 2005 Wooster Award (pdf, 0.4 Mb) Korea and U.S. federate metadata collection (pdf, 0.3 Mb) PICES Interns (pdf, 0.3 Mb) Studies on long-term variation of ocean ecosystem / climate interactions based on the Odate collection (pdf, 0.2 Mb) Hokkaido University Fisheries & Oceanographic Database CD-ROM (pdf, 0.2 Mb) Workshop on sardine and anchovy fluctuations (pdf, 0.1 Mb) Photo highlights of PICES XIV (pdf, 0.4 Mb) Workshop on SEEDS-II (pdf, 0.2 Mb) NPAFC-PICES joint symposium "The status of Pacific salmon and their role in North Pacific marine ecosystems" (pdf, 0.2 Mb) PICES Calendar (pdf, 0.2 Mb) New Chairman of the PICES Fishery Science Committee (pdf, 0.1 Mb) The state of the western North Pacific in the first half of 2005 (pdf, 0.4 Mb) Latest and upcoming PICES publications (pdf, 0.4 Mb) Recent trends in waters of the subarctic NE Pacific (pdf, 0.2 Mb) The Bering Sea: Current status and recent events (pdf, 0.1 Mb) PICES and GLOBEC to sponsor workshop on sub-arctic seas (pdf, 0.1 Mb) Professor Mikhail N. Koshlyakov - 75 (pdf, 0.1 Mb) Obituary - Dr. Al Tyler (pdf, 0.1 Mb)

PICES Press, Vol. 13, No. 1, January 2005

The state of PICES science - 2004 (pdf 0.7 MB) 2004 Wooster Award (pdf 0.2 MB) Micronekton – What are they and why are they important? (pdf 0.5 MB) Upscaling for a better understanding of climate links to ecosystems (pdf 0.1 MB) PICES Interns (pdf 0.1 MB) Report of the APN workshop on “Climate interactions and marine ecosystems” (pdf 0.6 MB) Photo highlights of PICES XIII (pdf 0.3 MB) Recent trends in waters of the subarctic NE Pacific – summer 2004 (pdf 0.1 MB) The state of the western North Pacific in the first half of 2004 (pdf 0.3 MB) The Bering Sea: Current status and recent events (pdf 0.1 MB) Study Group on Fisheries Ecosystem Responses to Recent Regime Shifts completes its mandate for the provision of scientific advice (pdf 0.1 MB) PICES Calendar (pdf 0.1 MB) The new PICES Working Group on Ecosystem-based management (pdf 0.05 MB) CO2 data integration activity for the North Pacific (pdf 0.2 MB) Carbon cycle changes in the North Pacific (pdf 0.8 MB) New and upcoming PICES publications (pdf 0.8 MB)

PICES Press, Vol. 12, No. 1, January 2004

The state of PICES science - 2003 (pdf 281 KB) 2003 Wooster Award (pdf 764 KB) The state of the eastern North Pacific through summer 2003 (pdf 448 KB) The Bering Sea: Current status and recent events (pdf 951 KB) The state of the western North Pacific in the first half of 2003 (pdf 684 KB) The status of oceanic zooplankton in the eastern North Pacific (pdf 390 KB) The precautionary approach to the PDO (pdf 976 KB) Photo highlights of PICES XII (pdf 2.79 MB) William G. Pearcy: Renaissance oceanographer (pdf 2.86 MB) KORDI/PICES/CoML Workshop on "Variability and status of the Yellow Sea and East China Sea ecosystems (pdf 785 KB) PICES/IOC Workshop on "Harmful algal blooms - Harmonization of data" (pdf 330 KB) From physics to predators: Monitoring North Pacific ecosystem dynamics (pdf 270 KB) Toward a coast-wide network of Northeast Pacific coastal-ocean monitoring programs - a brief workshop report (pdf 640) PICES publications (pdf 103 KB) PICES calendar (pdf 45 KB)

PICES Press, Vol. 11, No. 1, January 2003

Editorial and Contents The state of PICES science - 2002 Second annual Wooster Award to Yutaka Nagata Qingdao Open Science Meeting: A major landmark for GLOBEC GLOBEC OSM Session Highhlights PICES/GLOBEC Data Management Workshop PICES and GLOBEC modelling Some personal impressions of the GLOBEC OSM Photo highlights of PICES XI and GLOBEC OSM PICES Climate Change and Carrying Capacity (CCCC) Integration Workshop PICES/CLIVAR Workshop on Climate Variability in the Pacific and its impact on the marine ecosystem IGBP/SCOR Open Science Meeting on Ocean Biogeochemistry and Ecosystem Analysis Subarctic Ecosystem Response to Iron Enrichment Study (SERIES): eastern subarctic Pacific in July 2002 Introducing the GLOBEC International Project Office / GLOBEC Calendar 2003 Introducing the PICES Secretariat PICES Calendar

PICES Press, Vol. 9, No. 1, January 2001

The state of PICES science – 2000 [pp. 1-2] [pdf, 0.6 Mb] The state of the western North Pacific in the first half of 2000 [pp. 3-5] [pdf, 0.7 Mb] The status of the Bering Sea: January – August 2000 [pp. 6-7] [pdf, 0.3 Mb] The state of the eastern North Pacific since spring 2000 [pp. 8-9] [pdf, 0.4 Mb] Makoto Kashiwai [pp. 10-14] [pdf, 0.9 Mb] Alkalinity measurement quality improves for PICES nations [pp. 15-16] [pdf, 0.4 Mb] Dr. Timothy R. Parsons Awarded 2001 Japan Prize [p. 17] [pdf, 0.2 Mb] Highlights of the PICES Nineth Annual Meeting [pp. 18-19] [pdf, 0.4 Mb] Tangible outline of the whole elephant (Results of ecosystem studies of biological resources in the far-eastern seas in 1990s) [pp. 20-24] [pdf, 0.7 Mb] The Oshoro Maru: A short history of Hokkaido University's workhorse in the North Pacific [pp. 25-28] [pdf, 0.4 Mb] Bering Sea and North Pacific Ocean Theme Page [pp. 29-30] [pdf, 0.3 Mb] PICES IX Japan/East Sea cruise [pp. 31-34] [pdf, 1.1 Mb] 35 PICES Wooster Award [p. 35] [pdf, 0.2 Mb] PICES Intern Program [p. 35] [pdf, 0.2 Mb] Obituary - Prof. Michael M. Mulin [p. 36] [pdf, 0.2 Mb]

Evaluation of Several Commercial Algicides for Control of Odor-producing Cyanobacteria

The production of certain odorous metabolites is an undesirable attribute of cyanobacteria (blue-green algae) growth in aquaculture ponds [e.g., channel catfish(Ictalurus punctatus)] and in drinking water reservoirs. The most common odorous compounds encountered in catfish aquaculture are geosmin (trans-1,10-dimethyltrans-9-decalol) and 2-methylisoborneol(exo-1,2,7,7-tetramethylbicyclo[2.2.1]heptan-2-ol). These compounds are also frequently encountered worldwide in reservoirs and aqueducts used for municipal drinking water systems(Schrader et al. 2002). In this study, several algicides were evaluated using a rapid bioassay to determine their effectiveness in controlling the MIB-producing cyanobacterium Oscillatoria perornata from a west Mississippi catfish pond and the MIBproducing Pseudanabaena sp. (strain LW397) from Lake Whitehurst, Virginia, used as a city water supply reservoir. The cyanobacterium Oscillatoria agardhii , not a MIB-producer, and the green alga Selenastrum capricornutum , found in catfish ponds in the southeastern United States, were included in the bioassay to help determine potential broad-spectrum toxicity of the commercial products. (PDF has 3 pages.)

Tilapia for Biological Control of Giant Salvinia

In August 1999, giant salvinia ( Salvinia molesta Mitchell) was found along the lower Colorado River in irrigation drainages. To investigate the slow spread and apparent control of giant salvinia in this region, the herbivorous fish, tilapia (Oreochromis niloticus Trewavas), was examined as a biological control agent. The study was conducted in a 5,000-L recirculating system. (PDF contains 4 pages.)

Forecasting Climate Impacts on Future Production of Commercially Exploited Fish and Shellfish

Foreword [pdf, < 0.1 MB] Acknowledgements PHASE 1 [pdf, 0.2 MB] Summary of the PICES/NPRB Workshop on Forecasting Climate Impacts on Future Production of Commercially Exploited Fish and Shellfish (July 19–20, 2007, Seattle, U.S.A.) Background Links to Other Programs Workshop Format Session I. Status of climate change scenarios in the PICES region Session II. What are the expected impacts of climate change on regional oceanography and what are some scenarios for these drivers for the next 10 years? Session III. Recruitment forecasting Session IV. What models are out there? How is climate linked to the model? Session V. Assumptions regarding future fishing scenarios and enhancement activities Session VI Where do we go from here? References Appendix 1.1 List of Participants PHASE 2 [pdf, 0.7 MB] Summary of the PICES/NPRB Workshop on Forecasting Climate Impacts on Future Production of Commercially Exploited Fish and Shellfish (October 30, 2007, Victoria, Canada) Background Workshop Agenda Forecast Feasibility Format of Information Modeling Approaches Coupled bio-physical models Stock assessment projection models Comparative approaches Similarities in Data Requests Opportunities for Coordination with Other PICES Groups and International Efforts BACKGROUND REPORTS PREPARED FOR THE PHASE 2 WORKSHOP Northern California Current (U.S.) groundfish production by Melissa Haltuch Changes in sablefish (Anoplopoma fimbria) recruitment in relation to oceanographic conditions by Michael J. Schirripa Northern California Current (British Columbia) Pacific cod (Gadus macrocephalus) production by Caihong Fu and Richard Beamish Northern California Current (British Columbia) sablefish (Anoplopoma fimbria) production by Richard Beamish Northern California Current (British Columbia) pink (Oncorhynchus gorbuscha) and chum (O. keta) salmon production by Richard Beamish Northern California Current (British Columbia) ocean shrimp (Pandalus jordani) production by Caihong Fu Alaska salmon production by Anne Hollowed U.S. walleye pollock (Theragra chalcogramma) production in the eastern Bering Sea and Gulf of Alaska by Kevin Bailey and Anne Hollowed U.S. groundfish production in the eastern Bering Sea by Tom Wilderbuer U.S. crab production in the eastern Bering Sea by Gordon H. Kruse Forecasting Japanese commercially exploited species by Shin-ichi Ito, Kazuaki Tadokoro and Yasuhiro Yamanka Russian fish production in the Japan/East Sea by Yury Zuenko, Vladimir Nuzhdin and Natalia Dolganova Chum salmon (Oncorhynchus keta) production in Korea by Sukyung Kang, Suam Kim and Hyunju Seo Jack mackerel (Trachurus japonicus) production in Korea by Jae Bong Lee and Chang-Ik Zhang Chub mackerel (Scomber japonicus) production in Korea by Jae Bong Lee, Sukyung Kang, Suam Kim, Chang-Ik Zhang and Jin Yeong Kim References Appendix 2.1 List of Participants PHASE 3 [pdf, < 0.1 MB] Summary of the PICES Workshop on Linking Global Climate Model Output to (a) Trends in Commercial Species Productivity and (b) Changes in Broader Biological Communities in the World’s Oceans (May 18, 2008, Gijón, Spain) Appendix 3.1 List of Participants Appendix 3.2 Workshop Agenda (Document contains 101 pages)

Influence of seagrass landscape structure on the juvenile blue crab habitat-survival function

Organismal survival in marine habitats is often positively correlated with habitat structural complexity at local (within-patch) spatial scales. Far less is known, however, about how marine habitat structure at the landscape scale influences predation and other ecological processes, and in particular, how these processes are dictated by the interactive effect of habitat structure at local and landscape scales. The relationship between survival and habitat structure can be modeled with the habitat-survival function (HSF), which often takes on linear, hyperbolic, or sigmoid forms. We used tethering experiments to determine how seagrass landscape structure influenced the HSF for juvenile blue crabs Callinectes sapidus Rathbun in Back Sound, North Carolina, USA. Crabs were tethered in artificial seagrass plots of 7 different shoot densities embedded within small (1 – 3 m2) or large (>100 m2) seagrass patches (October 1999), and within 10 × 10 m landscapes containing patchy (<50% cover) or continuous (>90% cover) seagrass (July 2000). Overall, crab survival was higher in small than in large patches, and was higher in patchy than in continuous seagrass. The HSF was hyperbolic in large patches and in continuous seagrass, indicating that at low levels of habitat structure, relatively small increases in structure resulted in substantial increases in juvenile blue crab survival. However, the HSF was linear in small seagrass patches in 1999 and was parabolic in patchy seagrass in 2000. A sigmoid HSF, in which a threshold level of seagrass structure is required for crab survival, was never observed. Patchy seagrass landscapes are valuable refuges for juvenile blue crabs, and the effects of seagrass structural complexity on crab survival can only be fully understood when habitat structure at larger scales is considered.

Proceedings fo the Seventeenth Annual Sea Turtle Symposium, 4-8 March 1997, Orlando, Florida, U.S.A.

The 17th Annual Sea Turtle Symposium was held at the Delta Orlando Resort in Orlando, Florida U.S.A. from March 4-8, 1997. The symposium was hosted by Florida Atlantic University, Mote Marine Laboratory, University of Central Florida, University of Florida, Florida Atlantic University and the Comité Nacional para la Conservación y Protección de las Totugas Marinas. The 17th was the largest symposium to date. A total of 720 participants registered, including sea turtle biologists, students, regulatory personnel, managers, and volunteers representing 38 countries. In addition to the United States, participants represented Australia, Austria, the Bahamas, Bonaire, Bermuda, Brazil, Canada, Colombia, Costa Rica, Croatia, Cuba, Cyprus, Dominican Republic, Ecuador, England, Guatemala, Greece, Honduras, India, Italy, Japan, Madagascar, Malaysia, Mexico, The Netherlands, Nicaragua, Peru, Philippines, Republic of Seychelles, Scotland, Spain, Sri Lanka, Switzerland, Taiwan, Turkey, Uruguay, and Venezuela. In addition to the 79 oral, 2 video, and 120 poster presentations, 3 workshops were offered: Selina Heppell (Duke University Marine Laboratory) provided “Population Modeling,” Mike Walsh and Sam Dover (Sea World-Orlando) conducted “Marine Turtle Veterinary Medicine” and “Conservation on Nesting Beaches” was offered by Blair Witherington and David Arnold (Florida Department of Environmental Protection). On the first evening, P.C.H. Pritchard delivered a thoughtful retrospect on Archie Carr that showed many sides of a complex man who studied and wrote about sea turtles. It was a presentation that none of us will forget. The members considered a number of resolutions at the Thursday business meeting and passed six. Five of these resolutions are presented in the Commentaries and Reviews section of Chelonian Conservation and Biology 2(3):442-444 (1997). The symposium was fortunate to have many fine presentations competing for the Archie Carr Best Student Presentations awards. The best oral presentation award went to Amanda Southwood (University of British Columbia) for “Heart rates and dive behavior of the leatherback sea turtle during the internesting interval.” The two runners-up were Richard Reina (Australian National University) for “Regulation of salt gland activity in Chelonia mydas” and Singo Minamikawa (Kyoto University) for “The influence that artificial specific gravity change gives to diving behavior of loggerhead turtles”. The winner of this year’s best poster competition was Mark Roberts (University of South Florida) for his poster entitled “Global population structure of green sea Turtles (Chelonia mydas) using microsatellite analysis of male mediated gene flow.” The two runners-up were Larisa Avens (University of North Carolina-Chapel Hill) for “Equilibrium responses to rotational displacements by hatchling sea turtles: maintaining a migratory heading in a turbulent ocean” and Annette Broderick (University of Glasgow) for “Female size, not length, is a correlate of reproductive output.” The symposium was very fortunate to receive a matching monetary and subscription gift from Anders J. G. Rhodin of the Chelonian Research Foundation. These enabled us to more adequately reward the fine work of students. The winners of the best paper and best poster awards received $400 plus a subscription to Chelonian Conservation and Biology. Each runner up received $100. The symposium owes a great debt to countless volunteers who helped make the meeting a success. Those volunteers include: Jamie Serino, Alan Bolton, and Karen Bjorndal, along with the UF students provided audio visual help, John Keinath chaired the student awards committee, Mike Salmon chaired the Program Commiteee, Sheryan Epperly and Joanne Braun compiled the Proceedings, Edwin Drane served as treasurer and provided much logistical help, Jane Provancha coordinated volunteers, Thelma Richardson conducted registration, Vicki Wiese coordinated food and beverage services, Jamie Serino and Erik Marin coordinated entertainment, Kenneth Dodd oversaw student travel awards, Traci Guynup, Tina Brown, Jerris Foote, Dan Hamilton, Richie Moretti, and Vicki Wiese served on the time and place committee, Blair Witherington created the trivia quiz, Tom McFarland donated the symposium logo, Deborah Crouse chaired the resolutions committee, Pamela Plotkin chaired the nominations committee, Sally Krebs, Susan Schenk, and Larry Wood conducted the silent auction, and Beverly and Tom McFarland coordinated all 26 vendors. Many individuals from outside the United States were able to attend the 17th Annual Sea Turtle Symposium thanks to the tireless work of Karen Eckert, Marydele Donnelly, and Jack Frazier in soliciting travel assistance for a number of international participants. We are indebted to those donating money to the internationals’ housing fund (Flo Vetter Memorial Fund, Marinelife Center of Juno Beach, Roger Mellgren, and Jane Provancha). We raise much of our money for international travel from the auction; thanks go to auctioneer Bob Shoop, who kept our auction fastpaced and entertaining, and made sure the bidding was high. The Annual Sea Turtle Symposium is unequaled in its emphasis on international participation. Through international participation we all learn a great deal more about the biology of sea turtles and the conservation issues that sea turtles face in distant waters. Additionally, those attending the symposium come away with a tremendous wealth of knowledge, professional contacts, and new friendships. The Annual Sea Turtle Symposium is a meeting in which pretenses are dropped, good science is presented, and friendly, open communication is the rule. The camaraderie that typifies these meetings ultimately translates into understanding and cooperation. These aspects, combined, have gone and will go a long way toward helping to protect marine turtles and toward aiding their recovery on a global scale. (PDF contains 342 pages)

Hydroacoustic evaluation of spawning red hind (Epinephelus guttatus) aggregations along the coast of Puerto Rico in 2002 and 2003

Red hind (Epinephelus guttatus) have been overfished in the Caribbean and were included with seven other regional grouper species deemed vulnerable to risk of extinction. The Puerto Rico Department of Natural and Environmental Resources desired to map spawning red hind aggregations within commonwealth waters as part of their resource management program for the species. Mobile hydroacoustic surveys were conducted over 3-day periods in 2002 and 2003, indexed to the full moon phase in February or March when red hind were known to aggregate. Four vessels concurrently sampled the southwest, south, and southeast coasts of Puerto Rico in 2002. In 2003, three vessels conducted complementary surveys of the northwest, north, and northeast coasts of the island, completing a circuit of the coastal shelf-spawning habitat. These surveys indicated that red hind spawning aggregations were prevalent along the south and west coasts, and sparse along the north coast during the survey periods. Highest spawning red hind concentrations were observed in three areas offshore of the west coast of Puerto Rico, around Mona and Desecheo islands (20,443 and 10,559 fish/km2, respectively) and in the Bajo de Cico seasonal closed area (4,544 fish/km2). Following both 2002 and 2003 surveys, a series of controlled acoustic measurements of known local fish species in net pens were conducted to assess the mean target strength (acoustic backscatter) of each group. Ten species of fish were measured, including red hind (E. guttatus), coney (E. fulvus), white grunt (Haemulon plumieri), pluma (Calamus pennatula), blue tang (Acanthurus coeruleus), squirrel fish (Holocentrus spp.), black durgeon (Melichtyhs niger), ocean file fish (Canthidermis sufflamen), ocean surgeon fish (Acanthurus bahianus), and butter grouper (Mycteroperca spp.). In general, the mean target strength results from the caged fish experiments were in agreement with published target strength length relationships, with the exception of white grunt and pluma.

Sexual Reproduction and Early Development in the Estuarine Sea Anemone, Nematostella vectensis Stephenson, 1935

This dissertation: 1) determines the factor(s) responsible for spawning induction in NematosteJla vectensis; 2) isolates, describes, and documents the source of jelly from egg masses of N. vectensis; and 3) describes N. vectensis' early development. Namatostella vectensis were maintained on a 7-day mussel feeding/water change regime over 159 days. Within 36 hours of mussel feeding/water change. 69.1% of females and 78.5% of males spawned reliably. Through manipulation of feeding, water change, oxygen and nitrogenous waste concentrations, spawning induction was found to be triggered by the oxygen concentration associated with water change, and not by feeding. Ammonia, anemones' major waste product, inhibited this induction in a concentration-dependent manner. Female N. vectensis release eggs in a persistent jellied egg mass which is unique among the Actiniaria. The major component of this egg mass jelly was a positive periodic acid-Schiffs staining, 39.5-40.5 kD glycoprotein. Antibodies developed in rabbits against this glycoprotein bound to jelly of intact egg masses and to granules (~ 2.8 IJm in diameter) present in female anemone mesenteries and their associated filaments. Antibodies did not label male tissues. Nematostella vecfensis embryos underwent first karyokinesis -60 minutes following the addition of sperm to eggs. Second nuclear division took place, followed by first cleavage, 90-120 minutes later. Each of the 4 blastomeres that resulted from first cleavage contained a single nucleus. Arrangement of these blastomeres ranged from radial to pseudospiral. Embryonic development was both asynchronous and holoblastic. Following formation of the 4-cell stage, 71% of embryos proceeded to cleave again to form an 8-cell stage. In each of the remaining 29% of embryos, a fusion of from 2-4 blastomeres resulted in 4 possible patterns which had no affect on either cleavage interval timing or subsequent development. The fusion event was not due to ooplasmic segregation. Blastomeres isolated from 4-celled embryos were regulative and developed into normal planula larvae and juvenile anemones that were 1/4 the size of those that developed from intact 4-celled embryos. Embryos exhibiting the fusion phenomenon were examined at the fine structural level. The fusion phenomenon resulted in formation of a secondary syncytium and was not a mere compaction of blastomeres.