Study of white spot disease in four native species in Persian Gulf by histopathology and PCR methods

After serious disease outbreak, caused by new virus (WSV), has been occurring among cultured penaeid shrimps in Asian countries like China since 1993 and then in Latin American countries, during June till July 2002 a rapid and high mortality in cultured Penaeus indicus in Abadan region located in south of Iran with typical signs and symptoms of White Spot Syndrome Virus was confirmed by different studies of Histopathology, PCR, TEM, Virology. This study was conducted for the purpose of determination of prevalence(rate of infection)/ROI and grading severity (SOI) of WSD to five species: 150 samples of captured shrimps and 90 samples of cultured ones; Penaeus indicus, P. semisulcatus, P. merguiensis, Parapenaopsis styliferus, and Metapenaeus affinis in 2005. 136 of 240 samples have shown clinical and macroscopical signs & symptoms including; white spots on carapase (0.5-2 mm), easily removing of cuticule, fragility of hepatopancreas and red color of motility limbs. Histopathological changes like specific intranuclear inclusion bodies (cowdry-type A) were observed in all target tissues (gill, epidermis, haemolymph and midgut) but not in hepatopancreas, among shrimps collected from various farms in the south and captured ones from Persian Gulf, even ones without clinical signs. ROI among species estimated, using the NATIVIDAD & LIGHTNER formula(1992b) and SOI were graded, using a generalized scheme for assigning a numerical qualitative value to severity grade of infection which was provided by LIGHTNER(1996), in consideration to histopathology and counting specific inclusion bodies in different stages(were modified by B. Gholamhoseini). Samples with clinical signs, showed grades more than 2. Most of the P. semisulcatus and M. affinis samples showed grade of 3, in the other hand in most of P. styliferus samples grade of 4 were observed, which can suggest different sensitivity of different species. All samples were tested by Nested PCR method with IQTm 2000 WSSV kit and 183 of 240 samples were positive and 3 1evel of infection which was shown in this PCR confirmed our SOI grades, but they were more specified.

Polyculture: A boost to the Green Revolution Programme

Polyculture, as presently practiced in Nigeria, focuses interest mainly on finfish. This practice, apart from neglecting indigenous valuable species such as clams and water snails, does not make full use of existing biomass. This paper suggests possible deviations from normal practice. Inclusion of macroinvertebrates in a polyculture system and stocking, temporary bodies of water with seasonal species such as the fresh water crayfish are suggested. A final suggestion is to develop a polyculture of swamp rice and crayfish

Possible inclusion of fish waste (scales and fins) in the diet of fishes in aquaculture

The scales and fins of some freshwater fish species (Sarotherodon galilaeus, Oreochromis niloticus, Tilapia zillii, Barbus callipterus, Hemichromis fasciatus) from Kainji Lake, Nigeria were analyzed for their organic and inorganic matter. The aim of such study is to determine the usefulness of these waste parts of the fish in fish feed preparation. In all instances, the inorganic matter was found to be quite high in the fish exoskeleton, and calcium formed the highest element in the scales and the fins. These waste materials are therefore, considered as possible replacement for mineral sources in fish feed and probably the feed for other livestock

Sustainable management of water bodies for small-scale fisheries resorces research and development

Abstract The rapid growth of both formal and informal high density urban settlements around major water resources has led to increased pollution of streams, rivers, lakes and estuaries, due to contaminated runoff from these developments. The paper identified major contaminants to be : organic waste (sewage), industrial effluent, pesticides and litter. Pollutant loads vary depending on the hydrology of the urban area, local topography and soil conditions. In some instances, severe pollution of neighbouring and downstream water courses has been observed. The management of catchment land uses, riparian zones, in stream habitat, as well as in stream water flow patterns and quality are necessary in order to sustain the integrity and "health" of water resources, for fisheries and other developments. As such, attempts to ensure a certain level of water quality without attention to other aspects will not automatically ensure a "healthy" ecosystem even as fish habitat. Proper management leads to better water quality and conducive environment for increased fish production

Utilization of some aquatic macrophytes in Nigeria in-land water bodies: a review

Aquatic vegetation is an essential component of the aquatic ecosystem with both positive and negative implications on the water body. Efforts are always made to curtail the excessive growth of aquatic plants in order to prevent them from becoming a nuisance in the ecosystem. One of the ways of solving such problem is the positive economic use of such plants. Utilization as a method of weed control within the aquatic ecosystem is considered to be one of the safest methods of weed control as this provides the riparian communities double advantages in terms of save environment and personal benefits of the plant. The flora diversity of freshwater and brackish environments posses a great potential to both man and higher animals alike. Due to this fact, this paper attempt to review the exploited and unexploited aquatic plants resources of many of our water bodies in Nigeria both economica/ly and socially, to the populace. Recommendations are also advanced for further studies that will enhance sound management of the resources for maximum benefits and sustainability

Growth performance of Heteroclarias fed maggot meal at varying inclusion levels

70-day growth trial was conducted with Heteroclarias: Heterobranchus bidorsalis X Clarias gariepinus (mean weight 0.64~c0.006g) fed diets based on various inclusion levels of Maggot Meal. The fishmeal in the control diet was replaced with maggot meals at 25%, 50%, 75% and 100% levels to supply 40% crude protein in the final diets. The trails were conducted in glass tanks (60cmx30cmx30cm). Evaluation of growth parameters and nutrient utilization of the fish was based on weight gains, protein intake, protein efficiency ratio, net protein utilization, feed conversion efficiency and carcass analysis. Best growth and feed conversion efficiency were obtained with the 75% dietary inclusion of maggot meal. There was no significant differences (P>0.055) between the group of fish on 50% and 75% dietary inclusion maggot meal in growth performance and protein efficiency ratio but, there was a significant (P<0.05) difference in the NPU (Net Protein Utilization) and protein gain between the control diet and those fed on maggot meals. There was no marked variation in the survival rate of fish on all diets

Fish diversity of the wild and aquaculture water bodies in Singida Region

Tanzania Fisheries Research Institute being currently the only fisheries research institute in Tanzania has sole responsibility of marking the presence and distribution of fish species in the country. This work is expected to be completed by the year 2005. In order that this work can be accomplished, both published works and field observations (including geographical location using GPS) have to be compiled. Since the institute has centers in all the Great Lakes and one along the Indian Ocean coast, it is expected that field observations and identifications will be obtained and incorporated into the document to be produced by 2005. (PDF contains 15 pages)

On the ecology of species belonging to the genus Mesocyclops Sars (Crustacea, Copepoda) dwelling in the water-bodies of the Urals and Pri-Ural territory. [Translation from: Zoologicheskie Zhurnal 44(1) 127-130, 1965. ]

In the waterbodies of central Russia, the Urals and western Siberia four species of Crustacea, related to the genus Mesocyclops, are widely distributed: M. (s.str.) leuckarti (Claus), M. (Thermocyclops) oithonoides Sars, [M.](Th.) crassus (Fisch.) and M. (Th.) dybowskii (Lande). Numbers and biomass of Mesocyclops oithonoides in the pelagic water of various water-bodies of the Urals are presented and observations on the above mentioned species are discussed.

Radioautographic method of determination of number of bacteria in water-bodies [Translation from: Informatsionnyi Byulleten Biologiya Vnutrennikh Vod No.26, 65-67, 1975]

The radioautographic method of determination of the number of autotrophic microorganisms was initially suggested for counting methane-oxidizing bacteria. With the help of this method colonies of hydrogen-oxidizing bacteria are differentiated even more clearly from heterotrophic. Under laboratory conditions it was shown that colonies grown on membrane filters from a pure culture of thionic bacteria on a nutrient medium with radio- active carbonate, give better prints on film. This method was tested by the authors for determining the number of these bacteria in the meromictic Lake Vae de San Juan during the expedition to Cuba in the summer of 1973. The study showed that that the thionic bacteria are found throughout the pelagial. It proved that the thionic bacteria can be well considered in water-bodies by the radioautographic method.

Biology of the amphipod Gammarus pulex L. in water-bodies of the Leningrad district, and its piscicultural importance [Translation from: Trudy Vsesoyuznogo Gidrobiologicheskogo Obshchestva 7 99-122, 1956]

Observations and laboratory experiments are summarised that examine the widely discussed issue of what Gammarus pulex feeds on. On the basis of the observations of the authors and data from the literature it can be noted that G. pulex belongs to the omnivorous organisms, but, depending on the conditions of subsistence, in its rations either plant or animal food can predominate.

On the classification of lakes and lake-like bodies of the Ukraine [Translation from: Gidrobiologicheskie Zhurnal, Kiev 19(2) 100-101, 1983]

In the Ukraine there are several thousand large, medium and small lakes and lake-like reservoirs, distinguished by origin, salinity, regional position, productivity and by construction a significant number of large and small water bodies, ponds and industrial reservoirs of variable designation. The problem of national systems necessitates the creation of specific schemes and classifications. Classifying into specific types of reservoir by means of suitable specifications is required for planning national measures with the objective of the rational utilisation of natural resources. It is now necessary to consider the present-day characteristics of Ukranian lakes. In the case of the Ukraine it is possible to use two approaches - genetical and ecological. This paper uses the genetical system to classify the lake-like water bodies of the Ukraine.

Mathematical models for the prediction of temperature distributions resulting from the discharge of heated water into large bodies of water

Mathematical models for heated water outfalls were developed for three flow regions. Near the source, the subsurface discharge into a stratified ambient water issuing from a row of buoyant jets was solved with the jet interference included in the analysis. The analysis of the flow zone close to and at intermediate distances from a surface buoyant jet was developed for the two-dimensional and axisymmetric cases. Far away from the source, a passive dispersion model was solved for a two dimensional situation taking into consideration the effects of shear current and vertical changes in diffusivity. A significant result from the surface buoyant jet analysis is the ability to predict the onset and location of an internal hydraulic jump. Prediction can be made simply from the knowledge of the source Froude number and a dimensionless surface exchange coefficient. Parametric computer programs of the above models are also developed as a part of this study. This report was submitted in fulfillment of Contract No. 14-12-570 under the sponsorship of the Federal Water Quality Administration.

Small water bodies for sustainable fisheries production

A brief account is given of some of the discussions presented at a workshop regarding reservoir fisheries in Asia held in Hangzhou, China in Oct 1990. The presentations covered the range of reservoir fisheries management options, from basic limnological research and capture fisheries to intensive cage culture. Particular reference is made to the situation in China, describing current techniques used for stocking and developing reservoir fisheries.