Coral Disease and Health Workshop: Coral Histopathology II

The health and continued existence of coral reef ecosystems are threatened by an increasing array of environmental and anthropogenic impacts. Coral disease is one of the prominent causes of increased mortality among reefs globally, particularly in the Caribbean. Although over 40 different coral diseases and syndromes have been reported worldwide, only a few etiological agents have been confirmed; most pathogens remain unknown and the dynamics of disease transmission, pathogenicity and mortality are not understood. Causal relationships have been documented for only a few of the coral diseases, while new syndromes continue to emerge. Extensive field observations by coral biologists have provided substantial documentation of a plethora of new pathologies, but our understanding, however, has been limited to descriptions of gross lesions with names reflecting these observations (e.g., black band, white band, dark spot). To determine etiology, we must equip coral diseases scientists with basic biomedical knowledge and specialized training in areas such as histology, cell biology and pathology. Only through combining descriptive science with mechanistic science and employing the synthesis epizootiology provides will we be able to gain insight into causation and become equipped to handle the pending crisis. One of the critical challenges faced by coral disease researchers is to establish a framework to systematically study coral pathologies drawing from the field of diagnostic medicine and pathology and using generally accepted nomenclature. This process began in April 2004, with a workshop titled Coral Disease and Health Workshop: Developing Diagnostic Criteria co-convened by the Coral Disease and Health Consortium (CDHC), a working group organized under the auspices of the U.S. Coral Reef Task Force, and the International Registry for Coral Pathology (IRCP). The workshop was hosted by the U.S. Geological Survey, National Wildlife Health Center (NWHC) in Madison, Wisconsin and was focused on gross morphology and disease signs observed in the field. A resounding recommendation from the histopathologists participating in the workshop was the urgent need to develop diagnostic criteria that are suitable to move from gross observations to morphological diagnoses based on evaluation of microscopic anatomy. (PDF contains 92 pages)

Chaetognatha of the Caribbean Sea and adjacent areas

This illustrated manual is a guide to the distribution and identification of the 6 genera and 28 species of benthic and planktonic Chaetognatha known to occur in the Caribbean Sea, the Gulf of Mexlco, the Florida Straits, and the southwestern North Atlantic Ocean. As background, previous studies of chaetognaths in these areas are reviewed, gross morphology of the different forms is described, and instructions on methods of preserving and handling specimens preparatory to identification are provided. The key to genera and species is preceeded by a discussion of chaetognath taxonomy. A description of each species, consisting of an abbreviated synonymy, a summary of taxonomically important morphological features, and horizontal and vertical distribution follows the key. The occurrence of species in relation to water masses in the Caribbean and adjacent areas is noted. (PDF file contains 39 pages.)

Functional morphology of cephalopod gills

There is a wealth of literature dealing with fish gills (Review, see Hoar & Randall, 1984), yet hardly anything is known about the gills of cephalopods. This is rather surprising considering the commercial importance of the cephalopods. In view of the paucity of information available it was necessary to start by establishing the morphology of the gills. This is covered in the first section of this thesis. Of all the cephalopods, Octopus vulgaris was singled out for more detailed investigation (see chapters 2 & 3) as its physiology is comparatively well understood (Wells, 1978). The gills of cephalopods are the major sites for respiratory gaseous exchange. It follows that their dimensions might be expected to govern their potential for absorbing oxygen. Section two deals with the morphometries of cephalopod gills, and predicted values are compared with physiological measurements of oxygen uptake for four representative The final section describes the physiological experiments I performed on octopuses. These experiments were designed to find out whether the animals could regulate the gills' potential to take up oxygen through changes to the gills themselves.

Distribution, relative abundance and developmental morphology of paralarval cephalopods in the Western North Atlantic Ocean

Paralarval and juvenile cephalopods collected in plankton samples on 21 western North Atlantic cruises were identified and enumerated. The 3731 specimens were assigned to 44 generic and specific taxa. This paper describes their spatial and temporal distributions and their developmental morphology. The smallest paralarvae recognized for a number of species are identified and illustrated. The two most abundant and most frequently collected taxa were identifiable to species based on known systematic characters of young, as well as on distribution of the adults. These were the neritic squids Loligo pealeii and Illex illecebrosus collected north of Cape Hatteras, both valuable fishery resources. Other abundant taxa included two morphotypes of ommastrephids, at least five species of enoploteuthids, two species of onychoteuthids, and unidentified octopods. Most taxa were distributed widely both in time and in space, although some seasonal and mesoscale-spatial patterns were indicated. The taxa that appeared to have distinct seasonal distribution included most of the neritic species and, surprisingly, the young of the bathypelagic cranchiids. In eight seasonal cruises over the continental shelf of the middle U.S. Atlantic states, neritic taxa demonstrated approximately the same seasonal patterns during two consecutive years. Interannual differences in the oceanic taxa collected on the shelf were extreme. The highest abundance and diversity of planktonic cephalopods in the oceanic samples were consistently found in the vicinity of the Gulf Stream. Only eight of the oceanic taxa appeared to have limited areal distributions, compared with twelve taxa that were found throughout the western North Atlantic regions sampled in this study. Many taxa, however, were not collected frequently enough to describe seasonal or spatial patterns. Comparisons with published accounts of other cephalopod surveys indicate both strengths and weaknesses in various sampling techniques for capturing the young of oceanic cephalopods. Enoploteuthids were abundant both in our study and in other studies using midwater trawls in several areas of the North Atlantic. Thus, this family probably is adequately sampled over its developmental range. In contrast, octopoteuthids and chtenopterygiids are rare in collections made by small to medium-sized midwater trawls but are comparatively common in plankton samples. For families that are relatively common in plankton samples, paralarval abundance, derived similarly to the familiar ichthyoplankton surveys of fisheries science, may be the most reliable method of gathering data on distribution and abundance. (PDF file contains 58 pages.)

A histopathologic evaluation of gross lesions excised from commercially important North Altantic marine fishes

Histopathologic studies of lesions found in commercially important North Atlantic marine fishes are uncommon. As part of a comprehensive Northeast Fisheries Center program ("Ocean Pulse") to evaluate environmental and resource health on the U.S. Continental Shelf from Cape Hatteras to Nova Scotia, grossly visible lesions of the gills, integument, muscle, and viscera of primarily bottom-dwelling fishes were excised and examined using light microscopy. Several gadid and pleuronectid fishes accounted for most of the lesions observed. Most pathological examinations were incidental to samples taken for age and growth determination and evaluation of predator/prey relationships. Several gadids, with either gill, heart, or spleen lesions, were sampled more intensively. Gill lesions principally affected gadids and were caused by either microsporidans or an unidentified oocyte-like cell. The majority of gastrointestinal lesions consisted of encapsulated or encysted larval worms or microsporidan-induced cysts. Few heart lesions were found. Integumental lesioos included ulcers, lymphocystis, and trematode metacercariae. Liver lesions almost always consisted of encapsulated or encysted larval helminths. Necrotic granulomata were seen in muscle and microsporidan-induced granulomata in spleen. Although not numerous, histologically interesting lesions were noted in integument, heart, liver, spleen, and muscle of several fish species. Histologic study of tissues excised from a variety of demersal and pelagic fishes from the eastern North Atlantic (France, Germany, Spain) revealed assorted integumental, renal, hepatic, and splenic lesions. Small sample size and non-random sampling precluded obtaining a meaningful quantitative estimate of the prevalence of the observed lesions in the population at risk; however, a useful census has been made of the types of lesions present in commercially important marine fishes. (PDF file contains 20 pages.)

Effects of a flash-flood on channel morphology and some insects of a small stony stream in the Peak District, Derbyshire

In this paper, some observations are made following a flash-flood that occurred in Stake Clough, a small tributary of the River Goyt, during the evening of 6 August 1996. The site was visited eight times between 8 August - 30 October 1996 to take samples and make observations on the stream. The flood scoured the bed of Stake Clough but more significantly, caused it to change course along the middle part of the floodplain. Initially after the flood, the numbers of insects in all stretches of the stream channel were low (100-200 m super(2)), but then gradually rose to population densities approaching ten times this figure. The benthos was dominated by the Chironomidae and also leuctrid stoneflies (Leuctra nigra, L. hippopus and L. inermis). On 8th August 1996, 12 mesh bags, each containing oak leaves, were placed in the stream and collected after 24 hours. These were also dominated by chironomids, and contained relatively high numbers of the caddis, Potamophylax cingulatus.

Sphaerotilus natans: description of the morphology, growth and systematics of [Translation from: Schr. Ver. Zellstoff u. Pap.-Chem. u. -Ing., 26, 20-37, 1956]

The morphology, increase and systematica of Sphaerotilus natans is studied and culture methods examined.

The use of otolith morphology to indicate the stock structure of common coral trout (Plectropomus leopardus) on the Great Barrier Reef, Australia

We investigated the use of otolith morphology to indicate the stock structure of an exploited serranid coral reef fish, Plectropomus leopardus, on the Great Barrier Reef (GBR), Australia. Otoliths were measured by traditional one-and two-dimensional measures (otolith length, width, area, perimeter, circularity, and rectangularity), as well as by Fourier analysis to capture the finer details of otolith shape. Variables were compared among four regions of the GBR separated by hundreds of kilometers, as well as among three reefs within each region, hundreds of meters to tens of kilometers apart. The temporal stability in otolith structure was examined by comparing two cohorts of fully recruited four-year-old P. leopardus collected two years before and two years after a signif icant disturbance in the southern parts of the GBR caused by a large tropical cyclone in March 1997. Results indicated the presence of at least two stocks of P. leopardus, although the structure of each stock varied depending on the cohort considered. The results highlight the importance of incorporating data from several years in studies using otolith morphology to discriminate temporary and possibly misleading signals from those that indicate persistent spatial structure in stocks. We conclude that otolith morphology can be used as an initial step to direct further research on groups of P. leopardus that have lived at least a part of their life in different environments.

Reported Trip Costs, Gross Revenues, and Net Returns for U.S. Atlantic Pelagic Longline Vessels

Logbook set and trip summary data (containing catch and cost information, respectively) collected by NOAA’s National Marine Fisheries Service (NMFS) were analyzed for U.S. pelagic longline vessels that participated in Atlantic fisheries in 1996. These data were augmented with vessel information from the U.S. Coast Guard. Mean fish weights and ex-vessel prices from NMFS observers and licensed seafood dealers, respectively, were used to estimate gross revenues. Comparisons revealed that net returns varied substantially by vessel size and fishing behavior (i.e. sets per trip, fishing location, season, and swordfish targeting). While the calculated economic effects of proposed regulations will depend on the descriptive statistic chosen for analysis, which itself depends on the type of analysis being conducted, results show that considering heterogeneity within this fleet can have a significant effect on predicted economic consequences.

Gross energy requirement in fishing operations

Energy is a key input into the fish harvesting process. Efficient use of energy helps in reducing operational costs and environmental impact, while increasing profits. Energy optimisation is an important aspect of responsible fishing as enunciated in the Code of Conduct for Responsible Fisheries. Gross Energy Requirement (GER) is the sum of all non-renewable energy resources consumed in making available a product or service and is expressed in energy units per physical unit of product or service delivered. GER is a measure of intensity of non-renewable resource use and it reflects the amount of depletion of earth’s inherited store of non-renewable energy in order to create and make available a product or service. In this study, GER in fish harvesting up to the point of landing is estimated in selected fish harvesting systems in the small-mechanised sectors of Indian fisheries and compared with reported results from selected non mechanised and motorised fishing systems to reflect the situation during 1997-1998. Among the fish harvesting systems studied, GER t fish-1 ranged from 5.54 and 5.91 GJ, respectively, for wooden and steel purse seiners powered by 156 hp engines; 6.40 GJ for wooden purse seiner with 235 hp engine; 25.18 GJ for mechanised gillnet/line fishing vessel with 89 hp engines; to 31.40 and 36.97 GJ, respectively, for wooden and steel trawlers powered by 99-106 hp engines.

Influence of hard bottom morphology on fish assemblages of the continental shelf off Georgia, southeastern USA

Various reef types worldwide have inconsistent relationships among fish assemblage parameters and benthic characteristics, thus there is a need to identify factors driving assemblage structure specific to each reef type and locale. Limestone ledges are known to be key habitats for bottom fish on the continental shelf of the southeastern USA, however, the specific factors that link them to fish assemblages have not been quantified. Bottom fishes and habitat characteristics on ledges were surveyed at a study site located centrally in the southeastern USA continental shelf. Species richness, diversity, abundance, and biomass of fish were higher at ledges than on flat bottom. Species richness, abundance, and biomass of fish were well explained by ledge variables including percent cover of sessile invertebrates, total height, and height of undercut recesses. Multivariate analyses based on biomass of individual species at ledges revealed two fish assemblages associated with four ledge types. One assemblage was associated with ledges that were tall, heavily colonized with sessile invertebrates, large in area, and did or did not have undercuts. The other assemblage was associated with ledges that were short, not undercut, smaller in area, and were or were not heavily colonized by invertebrates. Seafloor classification schemes presently used in the region do not adequately capture hard bottom diversity to identify the location and extent of essential fish habitats for ecological and fisheries purposes. Given that ledges cover only ∼1% to 5% of the southeastern USA continental shelf, they merit the highest levels of consideration in regional research, conservation, and management plans.