Evaluación del rendimiento pesquero potencial de la República Argentina: III. Edad y crecimiento de peces 1

Age and growth of populations of three fish species from sixteen lakes and reservoirs situated in the Patagonian Andean and the Patagonian Plateau Region (Argentina) were studied. They included two native species, the Patagonian smallmouth perch. (Percichthys trucha) and the Patagonian silverside (Patagonina hatcheri) and the introduced rainbow trout (Oncorhynchus mykiss). For the three species backcalculated lenght at age was obtained from scale readings. Von Bertalanffy growth curves were usually adjusted to data. For the three species, faster growth was related with lake productivity. (Document contains 38 pages.)

Gegenüberstellung von mathematisch-physikalischer Modellierung und Messungen an geschleppten fischereitechnischen Systemen

When drafting a new model of a towed technical system for fisheries (trawl, towed TV gear or similar), and also when measuring an existing and already tested system it is not easy to foresee of practical behaviour, which depends of various parameters. The measuring programme for recording all data needs a lot of time and money, and also has some limitations. Therefore we developed for such systems mathematical-physical models, which allow a complex calculation. Their real value, however, results only from practical verification. During the cruise no. 222 of “Walther Herwig III” in November 2000 comparative investigations for 2concrete systems were carried out. This was done in cooperation with the University in Rostock, where such models are being developed and computerized. One of the systems mesasured was a pelagic herring trawl and the other one the towed TV gear for underwater observations of the Institute for Fishery Technology and Fish Quality. The correspondence between model calculation and measurements was very high for both systems.

Sensibilität von Seefischen an Bord. Teil 2: Demersale und pelagische Fischarten aus Schleppnetzfängen in der Nord- und Ostsee

The sensitiveness of different demersal and pelagic fish species of 70 hauls in the North and Baltic Sea in water depths of 60 to 250 m and 15 to 80 m, respectively, amount of catch of 100 to 3500 kg and trawling times of 0,5 to 6 h on board of the FRV “Walther Herwig III” was investigated. Some demersal fish species , e.g. saithe (Pollachius virens), were even still sensitive, when caught at a water depth of 250 m at a trawling time of 1,5 h. Generally the number of sensitive fishes was reduced with increasing water depth, amount of catch, trawling time and following storage of the catch on board. Among demersal fishes the species without swimbladder and flat fishes were clearly more resistent to mechanical stress. On the contrary, pelagic fish species were generally less robust. After trawling times of 2 h no sensitive animals were observed. In some fisheries there are mixed catches of demersal and pelagic fish species with different sensitiveness. In commercial fisheries, there is therefore – under animal welfare aspects – for the time being, no prospect for an improvement of the catching and slaughtering procedure on board

Einfluß von Eis- und Tiefgefrierlagerung/Auftauen auf die Länge von Seefischfilets

The objective was to study the influence of biological and processing conditions on the evaluation of a conversion factor between fillet length and fish size. This paper presents changes of the length and weight of skinless fillets from various marine fish species in relation to the ice storage time and to the deep freezing / thawing process. The biochemical changes during rigor mortis lead to considerable reduction in length of skinless fillets during ice storage and deep freezing / thawing of fillet blocks. The investigations were undertaken during various cruises of the FFS „Walther Herwig III“.

Vergleich von Ausbeutefaktoren und Eislagerqualität zwischen Kliesche (Limanda limanda) und Scholle (Pleuronectes platessa) aus der Nordsee

North Sea plaice (Pleuronectes platessa) and dab (Limanda limanda) were experimentally stored in ice for 6 days during the 181th cruise of the FRV “Wather Herwig III”. It could be demonstrated that both flat fish species showed the same storage properties and were of a comparable quality until the end of the storage experiment. The quality of both species was determined by sensory assessment of the quality grade, by measuring of the impedance using the German Fischtester VI and the Icelandic RT- tester and pH- and TVB- N- measurements. The average length of North Sea dab is generally small (female: 18,5 ± 3,9 cm; male: 17 ± 2,9 cm), therefore it seemed to be more efficient to process fish portions (eviscerated, head, tail, fins and part of belly flaps removed). The yield by manually processed filets or fish portions from dab is about 30 or 62 %, respectively

Sensorische Bewertung, Mikrobiologie und chemische Kenngrößen von eisgelagertem Wittling (Merlangius merlangus)

During the 160th research cruise of the FRV "Walther Herwig III" in the North Sea in May 1995 an ice-storage experiment with whiting was performed. Gutted whiting with and without spleen was stored in melting water-ice. Freshness and/or spoilage were monitored by measuring sensory, chemical, physical and microbiological indicators. It was found that besides the classical sensory assessment on the cooked sample and the EU-quality grading scheme, the microbiological counts were of major importance for the determination of the degree of freshness or spoilage. The cfu (colony forming units) of spoilage bacteria on the skin correlated significantly with time in ice. A very good correlation was also found for the cfu of spoilage bacteria with the sensory assessed odour of the cooked fillet sample. The measurement of the fish tissue with the Intellectron Fischtester VI and the determination of the creatine content in fillet are both suitable freshness and spoilage indicators. The pH-value measured in different body compartments and in musele homogenate and the ammonia content are only of limited value for freshness determination. Removal of kidney did not influence the shelf life.

Unerwartet niedrige Gehalte an toxischen Schwermetallen im Filet von Seefischen aus der Barentssee

Die 150. Reise des FFS "Walther Herwig III" wurde im Juli/August 1994 als Gemeinschaftsprojekt der Institute für Biochemie und Technologie und dem Institut für Fischereiökologie durchgeführt, um einen Überblick über die Schadstoffbelastung (anorganische und organische Schadstoffe, Radioaktivität) von Wasser, Sediment und Biota in der Barentssee zu erhalten. Das Institut für Biochemie und Technologie hat in diesem Forschungsprogramm den Teil "Bestimmung der Schwermetallgehalte im verzehrbaren Anteil (Filet) von Fischen und anderen Meerestieren" übernommen.

Differenzierung von Pangasius (Pangasius hypophthalmus) und Asiatischem Rotflossenwels (Hemibagrus wyckioides) durch Protein- und DNA-Analyse

In the last years farmed Pangasius (Tra-Pangasius, Pangasius hypophthalmus) from Vietnam has reached a considerable market share, whereas aquaculture of Asian Redtail Catfish (Hemibagrus wyckioides) is in its infancy. Recently it has been detected by food control authorities in Hamburg, that Pangasius fillets have been mislabelled and sold as fillets produced from Asian Redtail catfish. The necessity to improve the analytical methods for differentiation of Pangasius and Redtail Catfish prompted us to evaluate the suitability of isoelectric focusing (IEF) and DNA-analysis for identification of the two species. IEF of water soluble proteins was found to be a fast, reliable and economical method for differentiation of raw fillets of Pangasius and Redtail Catfish, as long as reference material is available. PCR-based DNA analysis was performed as follows: (i) amplification of a 464 bp segment of the cytochrome b gene; (ii) sequencing of the PCR product; (iii) comparison of the sequence with entries in GenBank using BLAST. The sequences of both species differed considerably, allowing the unequivocal differentiation between P. hypophthalmus and H. wyckioides. Kurzfassung Pangasius (Schlankwels, Tra-Pangasius, Pangasius hypophthalmus) hat sich innerhalb weniger Jahre zu einem bedeutenden Zuchtfisch entwickelt, während die Aquakultur des Asiatischen Rotflossenwelses (Hemibagrus wyckioides) in Vietnam noch in einem relativ kleinen Maßstab stattfindet. Kürzlich wurde von der Lebensmittelüberwachung in Hamburg nachgewiesen, dass im Handel erhältliche Filets mit der Deklaration „Rotflossenwels“ aus Pangasius hergestellt worden waren. Vor diesem Hintergrund wurden zwei Methoden auf ihre Eignung zur Differenzierung von Pangasius und Rotflossenwels geprüft. Es zeigte sich, dass sowohl die isoelektrische Fokussierung (IEF) wasserlöslicher Proteine als auch die PCR-basierte DNA-Analyse zur Unterscheidung beider Arten gut geeignet ist. Die IEF stellt eine schnelle und kostengünstige Untersuchungsmethode dar, die allerdings Referenzmaterial benötigt. Mit Hilfe der PCR (Polymerase-Kettenreaktion) wurde ein Abschnitt des Cytochrom b-Gens vervielfältigt und sequenziert. Die Sequenzen von P. hypophthalmus und H. wyckioides wiesen beträchtliche Unterschiede auf. Es wird diskutiert, wie sich durch Vergleich dieser Sequenzen mit Einträgen in Gendatenbanken unbekannte Proben beider Arten sicher zuordnen lassen.