Effects of iso-nitrogenous fertilizers as nutrient sources on carp polyculture in Bangladesh

A 120-day long experiment was conducted to find out the effects of urea plus triple super phosphate (UT), cow manure (CM) and poultry manure (PM) having iso-nil:rogen content on pond productivity and fish yield. Three fertilizer treatments, with three replicates each, were randomly assigned into nine earthen ponds of 100 m2 each. The stocking fish were rohu (Labeo ruhita), catla ( Catla catla) and mrigal ( Cirrhinus mrigala) in each treatment pond at the rate of 10000/ha with the ratio of 1:1:1. All ponds were fertilized fortnightly at the rate of 125 kg/ha urea plus 100 kg TSP/ha, 7000 kg/ha cow manure and 3500 kg/ha poultry manure for the treatment of UT, CM and PM, respectively, having an iso-nitrogen content of 56 kg in each. Though the physicochemical water quality parameters were more or less similar in all treatment ponds, the chlorophyll-a content and abundance of total plankton were significantly higher (P < 0.05) in the ponds receiving the treatment PM. Final growth as well as per unit production of fish was significantly higher (pof PM (2067 kg/ha/4 months) followed by UT (1639 kg/ha/4 months) and CM (1246 kg/ha/4 months). The overall results showed that the poultry manure proved to be superior to urea plus TSP and cow manure, even when nitrogen content is similar, in carp polyculture system under prevailing conditions.

Ice storage characteristics of fresh and brined shark fillets

Ice storage characteristics of fresh and brined fillets from fresh shark (Carcharias melanopterus) were studied in and out of contact with ice for more than two weeks. Changes occurring in biochemical constituents, physical qualities and bacterial counts of the fillets are reported. Shelf life of brined fillets out of contact with ice was considerably longer than that of control samples tinder similar conditions. Icing of shark fillets is suggested as a method for the removal of urea on a commercial scale.

Study of nephrocalcinosis in rainbow trout (Oncorhynchus mykiss), from fry to brood stock

For survey incidence of nephrocalcinosis in rainbow trout fish, during winter 1385, samplings were performed in three fish farm with different water source: river, spring and recirculation system by using well water. In this survey 5 specimens from each 8 groups and in general 120 specimens of this fish were caught by random sampling, and also amounts of O2, CO2, pH and temperature of water were measured. Then blooding and renal tissue sampling performed that renal samples were fixed in 10% buffered formalin and blood samples after separating serum stored in -200C. Renal specimens transferred to pathology laboratory, pathological slides were prepared and stained by hematoxylin & Eosin method. From 120 specimens, 6 cases of fish represent nephrocalcinosis. Pathologic signs include: renal epithelial necrosis, dilated ureters, dense basophilic materials inside the dilated tubules and cast formation in some renal tubules. From 6 cases of nephrocalcinosis, 3 cases (7/5%) were related to recirculation system and 2 (5%) case were related to river water and 1 (2/5%) case was related to spring water. In survey amount of urea, creatinine and Uric acid between different weight groups, distinguished that difference between creatinine middling in different weight groups were significant and also in between healthy and afflicted fish, significant statistical difference were only in creatinine amount between healthy and afflicted fish in each farm. Amounts of O2, pH and temperature of water in three farms were in normal range and only rate of water CO2 in ponds of recirculatory system were very higher (25 mg/lit) than other farms. May be, this reason led to high number of nephrocalcinosis in recirculatory system, than other farms, nemley 7/5% of fish that caught from this farm .This subject is related to the role of CO2 in creating nephrocalcinosis which is descript in references.

Protein concentrate from shark

A simple method of leaching the minced muscle with water repeatedly followed by cooking, pressing, drying the cake and powdering has been described for the preparation of fish protein concentrate (FPC) from shark without the use of solvents. The FPC thus prepared had high protein content and was completely free of urea. It contained all the essential amino acids in a balanced proportion with high lysine content and had a storage life up to 12 months. This product can be used for the fortification of bread, biscuits and chappathis respectively at 10, 5 and 2% levels.